CXCL16 Haplotypes in Patients with Human Carotid Atherosclerosis: Preliminary Results
Samo za registrovane korisnike
2015
Autori
Živković, MajaĐurić, Tamara
Stojković, Ljiljana S.
Jovanović, Ivan G.
Končar, Igor
Davidović, Lazar
Veljković, Nevena V.
Alavantić, Dragan
Stanković, Aleksandra
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Aim: Chemokine CXC ligand 16 (CXCL16) has chemoattractive, adhesive and scavenging properties and may play a role in the formation of atherosclerotic lesions. However, studies of CXCL16 polymorphisms in patients with atherosclerosis are scarce. The missense polymorphisms I123T and A181V are potentially important factors in the regulation of presentation and shedding of the CXCL16 chemokine domain. The aim of this study was to analyze the association between I123T and A181V polymorphism haplotypes and the accumulation of carotid plaque as well as the effect of the haplotype on the CXCL16 mRNA expression in carotid plaques in patients with advanced atherosclerosis. Additionally, we performed a bioinformatic prediction analysis of the impact of CXCL16 protein sequence variation on CXCL16-CXCR6 interactions and analyzed the soluble CXCL16 plasma levels according to the CXCL16 haplotype. Methods: This study evaluated a total of 733 participants, including 283 controls and 450 patients with ...carotid atherosclerosis (CA) undergoing endarterectomy. Analyses of the polymorphisms and the gene expression were performed using real-time PCR. The soluble CXCL16 levels were measured with ELISA. Results: The missense allele haplotype, T123V181, was found to be significantly and independently associated with the occurrence of CA plaque (OR=1.27; 1.02-1.57, p=0.03). This haplotype was predicted to significantly change the CXCL16-CXCR6 interaction, compared to I123A181. Neither the CXCL16 mRNA expression in the human plaques nor the soluble CXCL16 plasma levels differed according to the haplotype. Conclusions: These results indicate that the CXCL16 T123V181 haplotype is a moderate genetic risk factor for the development of carotid plaque. Further functional and replication studies are needed to clarify the mechanisms by which this combination of alleles promotes advanced CA and validate its impact on disease progression.
Ključne reči:
CXCL16 / CXCR6 / Haplotype / Gene expression / Carotid plaqueIzvor:
Journal of Atherosclerosis and Thrombosis, 2015, 22, 1, 10-20Finansiranje / projekti:
- Integralna studija identifikacije regionalnih genetskih faktora rizika i faktora rizika životne sredine za masovne nezarazne bolesti humane populacije u Srbiji - INGEMA_S (RS-41028)
- Primena EIIP/ISM bioinformatičke platforme u otkrivanju novih terapeutskih targeta i potencijalnih terapeutskih molekula (RS-173001)
DOI: 10.5551/jat.24299
ISSN: 1340-3478; 1880-3873
PubMed: 25142184
WoS: 000352097800002
Scopus: 2-s2.0-84943240571
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Živković, Maja AU - Đurić, Tamara AU - Stojković, Ljiljana S. AU - Jovanović, Ivan G. AU - Končar, Igor AU - Davidović, Lazar AU - Veljković, Nevena V. AU - Alavantić, Dragan AU - Stanković, Aleksandra PY - 2015 UR - https://vinar.vin.bg.ac.rs/handle/123456789/481 AB - Aim: Chemokine CXC ligand 16 (CXCL16) has chemoattractive, adhesive and scavenging properties and may play a role in the formation of atherosclerotic lesions. However, studies of CXCL16 polymorphisms in patients with atherosclerosis are scarce. The missense polymorphisms I123T and A181V are potentially important factors in the regulation of presentation and shedding of the CXCL16 chemokine domain. The aim of this study was to analyze the association between I123T and A181V polymorphism haplotypes and the accumulation of carotid plaque as well as the effect of the haplotype on the CXCL16 mRNA expression in carotid plaques in patients with advanced atherosclerosis. Additionally, we performed a bioinformatic prediction analysis of the impact of CXCL16 protein sequence variation on CXCL16-CXCR6 interactions and analyzed the soluble CXCL16 plasma levels according to the CXCL16 haplotype. Methods: This study evaluated a total of 733 participants, including 283 controls and 450 patients with carotid atherosclerosis (CA) undergoing endarterectomy. Analyses of the polymorphisms and the gene expression were performed using real-time PCR. The soluble CXCL16 levels were measured with ELISA. Results: The missense allele haplotype, T123V181, was found to be significantly and independently associated with the occurrence of CA plaque (OR=1.27; 1.02-1.57, p=0.03). This haplotype was predicted to significantly change the CXCL16-CXCR6 interaction, compared to I123A181. Neither the CXCL16 mRNA expression in the human plaques nor the soluble CXCL16 plasma levels differed according to the haplotype. Conclusions: These results indicate that the CXCL16 T123V181 haplotype is a moderate genetic risk factor for the development of carotid plaque. Further functional and replication studies are needed to clarify the mechanisms by which this combination of alleles promotes advanced CA and validate its impact on disease progression. T2 - Journal of Atherosclerosis and Thrombosis T1 - CXCL16 Haplotypes in Patients with Human Carotid Atherosclerosis: Preliminary Results VL - 22 IS - 1 SP - 10 EP - 20 DO - 10.5551/jat.24299 ER -
@article{ author = "Živković, Maja and Đurić, Tamara and Stojković, Ljiljana S. and Jovanović, Ivan G. and Končar, Igor and Davidović, Lazar and Veljković, Nevena V. and Alavantić, Dragan and Stanković, Aleksandra", year = "2015", abstract = "Aim: Chemokine CXC ligand 16 (CXCL16) has chemoattractive, adhesive and scavenging properties and may play a role in the formation of atherosclerotic lesions. However, studies of CXCL16 polymorphisms in patients with atherosclerosis are scarce. The missense polymorphisms I123T and A181V are potentially important factors in the regulation of presentation and shedding of the CXCL16 chemokine domain. The aim of this study was to analyze the association between I123T and A181V polymorphism haplotypes and the accumulation of carotid plaque as well as the effect of the haplotype on the CXCL16 mRNA expression in carotid plaques in patients with advanced atherosclerosis. Additionally, we performed a bioinformatic prediction analysis of the impact of CXCL16 protein sequence variation on CXCL16-CXCR6 interactions and analyzed the soluble CXCL16 plasma levels according to the CXCL16 haplotype. Methods: This study evaluated a total of 733 participants, including 283 controls and 450 patients with carotid atherosclerosis (CA) undergoing endarterectomy. Analyses of the polymorphisms and the gene expression were performed using real-time PCR. The soluble CXCL16 levels were measured with ELISA. Results: The missense allele haplotype, T123V181, was found to be significantly and independently associated with the occurrence of CA plaque (OR=1.27; 1.02-1.57, p=0.03). This haplotype was predicted to significantly change the CXCL16-CXCR6 interaction, compared to I123A181. Neither the CXCL16 mRNA expression in the human plaques nor the soluble CXCL16 plasma levels differed according to the haplotype. Conclusions: These results indicate that the CXCL16 T123V181 haplotype is a moderate genetic risk factor for the development of carotid plaque. Further functional and replication studies are needed to clarify the mechanisms by which this combination of alleles promotes advanced CA and validate its impact on disease progression.", journal = "Journal of Atherosclerosis and Thrombosis", title = "CXCL16 Haplotypes in Patients with Human Carotid Atherosclerosis: Preliminary Results", volume = "22", number = "1", pages = "10-20", doi = "10.5551/jat.24299" }
Živković, M., Đurić, T., Stojković, L. S., Jovanović, I. G., Končar, I., Davidović, L., Veljković, N. V., Alavantić, D.,& Stanković, A.. (2015). CXCL16 Haplotypes in Patients with Human Carotid Atherosclerosis: Preliminary Results. in Journal of Atherosclerosis and Thrombosis, 22(1), 10-20. https://doi.org/10.5551/jat.24299
Živković M, Đurić T, Stojković LS, Jovanović IG, Končar I, Davidović L, Veljković NV, Alavantić D, Stanković A. CXCL16 Haplotypes in Patients with Human Carotid Atherosclerosis: Preliminary Results. in Journal of Atherosclerosis and Thrombosis. 2015;22(1):10-20. doi:10.5551/jat.24299 .
Živković, Maja, Đurić, Tamara, Stojković, Ljiljana S., Jovanović, Ivan G., Končar, Igor, Davidović, Lazar, Veljković, Nevena V., Alavantić, Dragan, Stanković, Aleksandra, "CXCL16 Haplotypes in Patients with Human Carotid Atherosclerosis: Preliminary Results" in Journal of Atherosclerosis and Thrombosis, 22, no. 1 (2015):10-20, https://doi.org/10.5551/jat.24299 . .