Oxidation of chlorpyrifos, azinphos-methyl and phorate by myeloperoxidase
Apstrakt
The present paper deals with the investigations of optimal conditions for the myeloperoxidase (MPO) mediated oxidation of chlorpyrifos, azinphos-methyl and phorate, organophosphorous pesticides (OPs) containing phosphorothionate group, from thio- to oxo-forms, in the presence of hydrogen peroxide. The aim of the work was to apply this oxidation method in the AChE based bioanalytical tests for OPs determination. The maximum concentration of oxo-forms for all tested pesticides was achieved after 10 min incubation of OPs in 50 mM phosphate buffer (pH 6.0) with 100 nM MPO in the presence of 50 mu M H(2)O(2). Optimal temperature for obtaining maximal concentration of oxo-forms was 37 degrees C. Only the parent compounds and their oxo-forms were identified chromatographically in the OPs samples after their exposure to MPO. Moreover, no hydrolysis products were detected in the time interval of 1 h after the MPO catalyzed reaction was stopped by catalase. The efficiency of OPs transformation f...rom thio- to oxo-forms was measured using acethylcholinesterase (AChE) test, by comparison of percent of AChE inhibition before and after exposure to the oxidized sample. (C) 2011 Elsevier Inc. All rights reserved.
Ključne reči:
Organophosphate / Pesticide / Oxidation / Myeloperoxidase / acetylcholinesteraseIzvor:
Pesticide Biochemistry and Physiology, 2011, 101, 3, 220-226Finansiranje / projekti:
- Istraživanja interakcija enzima sa toksičnim i farmakološki aktivnim molekulima (RS-MESTD-Basic Research (BR or ON)-172023)
DOI: 10.1016/j.pestbp.2011.09.009
ISSN: 0048-3575
WoS: 000297879600012
Scopus: 2-s2.0-81555204344
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Lazarević-Pašti, Tamara AU - Nastasijević, Branislav J. AU - Vasić, Vesna M. PY - 2011 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4597 AB - The present paper deals with the investigations of optimal conditions for the myeloperoxidase (MPO) mediated oxidation of chlorpyrifos, azinphos-methyl and phorate, organophosphorous pesticides (OPs) containing phosphorothionate group, from thio- to oxo-forms, in the presence of hydrogen peroxide. The aim of the work was to apply this oxidation method in the AChE based bioanalytical tests for OPs determination. The maximum concentration of oxo-forms for all tested pesticides was achieved after 10 min incubation of OPs in 50 mM phosphate buffer (pH 6.0) with 100 nM MPO in the presence of 50 mu M H(2)O(2). Optimal temperature for obtaining maximal concentration of oxo-forms was 37 degrees C. Only the parent compounds and their oxo-forms were identified chromatographically in the OPs samples after their exposure to MPO. Moreover, no hydrolysis products were detected in the time interval of 1 h after the MPO catalyzed reaction was stopped by catalase. The efficiency of OPs transformation from thio- to oxo-forms was measured using acethylcholinesterase (AChE) test, by comparison of percent of AChE inhibition before and after exposure to the oxidized sample. (C) 2011 Elsevier Inc. All rights reserved. T2 - Pesticide Biochemistry and Physiology T1 - Oxidation of chlorpyrifos, azinphos-methyl and phorate by myeloperoxidase VL - 101 IS - 3 SP - 220 EP - 226 DO - 10.1016/j.pestbp.2011.09.009 ER -
@article{ author = "Lazarević-Pašti, Tamara and Nastasijević, Branislav J. and Vasić, Vesna M.", year = "2011", abstract = "The present paper deals with the investigations of optimal conditions for the myeloperoxidase (MPO) mediated oxidation of chlorpyrifos, azinphos-methyl and phorate, organophosphorous pesticides (OPs) containing phosphorothionate group, from thio- to oxo-forms, in the presence of hydrogen peroxide. The aim of the work was to apply this oxidation method in the AChE based bioanalytical tests for OPs determination. The maximum concentration of oxo-forms for all tested pesticides was achieved after 10 min incubation of OPs in 50 mM phosphate buffer (pH 6.0) with 100 nM MPO in the presence of 50 mu M H(2)O(2). Optimal temperature for obtaining maximal concentration of oxo-forms was 37 degrees C. Only the parent compounds and their oxo-forms were identified chromatographically in the OPs samples after their exposure to MPO. Moreover, no hydrolysis products were detected in the time interval of 1 h after the MPO catalyzed reaction was stopped by catalase. The efficiency of OPs transformation from thio- to oxo-forms was measured using acethylcholinesterase (AChE) test, by comparison of percent of AChE inhibition before and after exposure to the oxidized sample. (C) 2011 Elsevier Inc. All rights reserved.", journal = "Pesticide Biochemistry and Physiology", title = "Oxidation of chlorpyrifos, azinphos-methyl and phorate by myeloperoxidase", volume = "101", number = "3", pages = "220-226", doi = "10.1016/j.pestbp.2011.09.009" }
Lazarević-Pašti, T., Nastasijević, B. J.,& Vasić, V. M.. (2011). Oxidation of chlorpyrifos, azinphos-methyl and phorate by myeloperoxidase. in Pesticide Biochemistry and Physiology, 101(3), 220-226. https://doi.org/10.1016/j.pestbp.2011.09.009
Lazarević-Pašti T, Nastasijević BJ, Vasić VM. Oxidation of chlorpyrifos, azinphos-methyl and phorate by myeloperoxidase. in Pesticide Biochemistry and Physiology. 2011;101(3):220-226. doi:10.1016/j.pestbp.2011.09.009 .
Lazarević-Pašti, Tamara, Nastasijević, Branislav J., Vasić, Vesna M., "Oxidation of chlorpyrifos, azinphos-methyl and phorate by myeloperoxidase" in Pesticide Biochemistry and Physiology, 101, no. 3 (2011):220-226, https://doi.org/10.1016/j.pestbp.2011.09.009 . .