Effect of Insulin on Adiponectin and Adiponectin Receptor-1 Expression in Rats with Streptozotocin-induced Type 2 Diabetes
2011
Autori
Al-Hashem, FahaidIbrahim, Ibrahim
Bastawy, Nermeen
Rateb, Moshira
Haidara, Mohamed A.
Dallak, Mohammed
Soskić, Sanja S.
Bin-Jaliah, Ismaeel
Isenović, Esma R.
Članak u časopisu
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The present study was designed to investigate the effect of insulin on insulin resistance (IR), plasma adiponectin level and expression of adiponectin receptors 1 (AdipoR1) in obese and streptozotocin-induced type 2 diabetic rats. Male Sprague-Dowley rats were randomized to control group and 3 obese experimental groups. Type 2 diabetic mellitus was induced in the 3(rd) and 4(th) experimental groups by given 30 mg/kg of a single dose streptozotocin via intraperitoneal injection (i.p.). Fourth group was treated with i.p. 1 IU insulin/kg/day for 6 days before end of the experiment which lasts for 8 weeks while same amount of normal saline was i.p. given to other group. At the end of the study (8 weeks), plasma levels of adiponectin, triglycerides (TO), cholesterol, fasting blood glucose and insulin were measured. Obesity index (01) and IR were calculated. AdipoR1 mRNA levels in the soleus muscle tissue were semi-quantitated. Hyperlipidemia, hyperinsulinemia and hyperglycemia were observed... ill both obese and diabetic rats, which were accompanied by hypoadiponectinemia and down regulation of AdipoR1 expression as compared to the control rats. Adiponectin was negatively correlated with all the biochemical parameters assessed. Insulin treatment significantly improved these metabolic abnormalities and effectively restored adiponectin and AdipoR1 to the control level. In conclusion, adiponectin and its receptor-associated cascade may be aberrantly regulated in both obesity and type 2 diabetes and targeting adiponectin and its receptors may offer a novel therapy against obesity and type 2 diabetes.
Ključne reči:
adiponectin / diabetes / streptozotocin / insulinIzvor:
Journal of Health Science, 2011, 57, 4, 334-340Finansiranje / projekti:
- Hormonska regulacija ekspresije i aktivnosti azot oksid sintaze i natrijum-kalijumove pumpe u eksperimentalnim modelima insulinske rezistencije, dijabetesa i kardiovaskularnih poremećaja (RS-MESTD-Basic Research (BR or ON)-173033)
- Kasr Al-Aini Research Centre, Ministry of Science, Republic of Serbia between King Khalid University in Saudi Arabia
DOI: 10.1248/jhs.57.334
ISSN: 1344-9702
WoS: 000294527600002
Scopus: 2-s2.0-79960993712
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Al-Hashem, Fahaid AU - Ibrahim, Ibrahim AU - Bastawy, Nermeen AU - Rateb, Moshira AU - Haidara, Mohamed A. AU - Dallak, Mohammed AU - Soskić, Sanja S. AU - Bin-Jaliah, Ismaeel AU - Isenović, Esma R. PY - 2011 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4472 AB - The present study was designed to investigate the effect of insulin on insulin resistance (IR), plasma adiponectin level and expression of adiponectin receptors 1 (AdipoR1) in obese and streptozotocin-induced type 2 diabetic rats. Male Sprague-Dowley rats were randomized to control group and 3 obese experimental groups. Type 2 diabetic mellitus was induced in the 3(rd) and 4(th) experimental groups by given 30 mg/kg of a single dose streptozotocin via intraperitoneal injection (i.p.). Fourth group was treated with i.p. 1 IU insulin/kg/day for 6 days before end of the experiment which lasts for 8 weeks while same amount of normal saline was i.p. given to other group. At the end of the study (8 weeks), plasma levels of adiponectin, triglycerides (TO), cholesterol, fasting blood glucose and insulin were measured. Obesity index (01) and IR were calculated. AdipoR1 mRNA levels in the soleus muscle tissue were semi-quantitated. Hyperlipidemia, hyperinsulinemia and hyperglycemia were observed ill both obese and diabetic rats, which were accompanied by hypoadiponectinemia and down regulation of AdipoR1 expression as compared to the control rats. Adiponectin was negatively correlated with all the biochemical parameters assessed. Insulin treatment significantly improved these metabolic abnormalities and effectively restored adiponectin and AdipoR1 to the control level. In conclusion, adiponectin and its receptor-associated cascade may be aberrantly regulated in both obesity and type 2 diabetes and targeting adiponectin and its receptors may offer a novel therapy against obesity and type 2 diabetes. T2 - Journal of Health Science T1 - Effect of Insulin on Adiponectin and Adiponectin Receptor-1 Expression in Rats with Streptozotocin-induced Type 2 Diabetes VL - 57 IS - 4 SP - 334 EP - 340 DO - 10.1248/jhs.57.334 ER -
@article{ author = "Al-Hashem, Fahaid and Ibrahim, Ibrahim and Bastawy, Nermeen and Rateb, Moshira and Haidara, Mohamed A. and Dallak, Mohammed and Soskić, Sanja S. and Bin-Jaliah, Ismaeel and Isenović, Esma R.", year = "2011", abstract = "The present study was designed to investigate the effect of insulin on insulin resistance (IR), plasma adiponectin level and expression of adiponectin receptors 1 (AdipoR1) in obese and streptozotocin-induced type 2 diabetic rats. Male Sprague-Dowley rats were randomized to control group and 3 obese experimental groups. Type 2 diabetic mellitus was induced in the 3(rd) and 4(th) experimental groups by given 30 mg/kg of a single dose streptozotocin via intraperitoneal injection (i.p.). Fourth group was treated with i.p. 1 IU insulin/kg/day for 6 days before end of the experiment which lasts for 8 weeks while same amount of normal saline was i.p. given to other group. At the end of the study (8 weeks), plasma levels of adiponectin, triglycerides (TO), cholesterol, fasting blood glucose and insulin were measured. Obesity index (01) and IR were calculated. AdipoR1 mRNA levels in the soleus muscle tissue were semi-quantitated. Hyperlipidemia, hyperinsulinemia and hyperglycemia were observed ill both obese and diabetic rats, which were accompanied by hypoadiponectinemia and down regulation of AdipoR1 expression as compared to the control rats. Adiponectin was negatively correlated with all the biochemical parameters assessed. Insulin treatment significantly improved these metabolic abnormalities and effectively restored adiponectin and AdipoR1 to the control level. In conclusion, adiponectin and its receptor-associated cascade may be aberrantly regulated in both obesity and type 2 diabetes and targeting adiponectin and its receptors may offer a novel therapy against obesity and type 2 diabetes.", journal = "Journal of Health Science", title = "Effect of Insulin on Adiponectin and Adiponectin Receptor-1 Expression in Rats with Streptozotocin-induced Type 2 Diabetes", volume = "57", number = "4", pages = "334-340", doi = "10.1248/jhs.57.334" }
Al-Hashem, F., Ibrahim, I., Bastawy, N., Rateb, M., Haidara, M. A., Dallak, M., Soskić, S. S., Bin-Jaliah, I.,& Isenović, E. R.. (2011). Effect of Insulin on Adiponectin and Adiponectin Receptor-1 Expression in Rats with Streptozotocin-induced Type 2 Diabetes. in Journal of Health Science, 57(4), 334-340. https://doi.org/10.1248/jhs.57.334
Al-Hashem F, Ibrahim I, Bastawy N, Rateb M, Haidara MA, Dallak M, Soskić SS, Bin-Jaliah I, Isenović ER. Effect of Insulin on Adiponectin and Adiponectin Receptor-1 Expression in Rats with Streptozotocin-induced Type 2 Diabetes. in Journal of Health Science. 2011;57(4):334-340. doi:10.1248/jhs.57.334 .
Al-Hashem, Fahaid, Ibrahim, Ibrahim, Bastawy, Nermeen, Rateb, Moshira, Haidara, Mohamed A., Dallak, Mohammed, Soskić, Sanja S., Bin-Jaliah, Ismaeel, Isenović, Esma R., "Effect of Insulin on Adiponectin and Adiponectin Receptor-1 Expression in Rats with Streptozotocin-induced Type 2 Diabetes" in Journal of Health Science, 57, no. 4 (2011):334-340, https://doi.org/10.1248/jhs.57.334 . .