Gender-specific response of brain corticosteroid receptors to stress and fluoxetine
Authorized Users Only
2011
Authors
Elaković, IvanaĐorđević, Ana D.
Adžić, Miroslav
Đorđević, Jelena D.
Radojčić, Marija
Matić, Gordana
Article (Published version)
Metadata
Show full item recordAbstract
Gender-related differences in dexamethasone binding to corticosteroid receptors (CR) and in glucocorticoid receptor (GR) protein level in the pituitary, hypothalamus, hippocampus and prefrontal cortex were studied before and after antidepressant fluoxetine administration to both unstressed and rats exposed to a chronic social isolation stress. Untreated males, in comparison to females, displayed higher hormone-binding capacity of both GR and mineralocorticoid receptor (MR) in the hippocampal cytosol, as well as higher GR protein level in the pituitary cytosol. In both genders, dexamethasone binding to MR exceeded that to GR. While fluoxetine treatment and social isolation had no effect on GR activity, the influence on MR was gender-specific. Fluoxetine facilitated MR hormone-binding only in females, increasing the MR/GA activity ratio. In contrast, after a 6-week isolation of males, MR binding capacity was diminished and MR/GR ratio inverted in favor of GR In addition, fluoxetine induc...ed elevation of cytosolic GR protein level in the pituitary and hypothalamus, the latter change being gender-specific. The results point to gender-related differences in the CRs functioning and suggest that both MR and GR may contribute to well-known sexual dimorphism in vulnerability to stress and stress-related disorders and in the outcome of antidepressant treatment. (C) 2011 Elsevier B.V. All rights reserved.
Keywords:
Glucocorticoid receptor / Mineralocorticoid receptor / Gender differences / Antidepressant fluoxetine / Social isolation stressSource:
Brain Research, 2011, 1384, 61-68Funding / projects:
- Role of steroid hormones in neuroendocrine adaptation to stress and pathophysiology of metabolic syndrome - molecular mechanisms and clinical implications (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41009)
- Defining a cluster of molecular biomarkers for improved diagnostics and therapy of mood disorders (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41029)
DOI: 10.1016/j.brainres.2011.01.078
ISSN: 0006-8993; 1872-6240
PubMed: 21281618
WoS: 000289543100008
Scopus: 2-s2.0-79953024327
Collections
Institution/Community
VinčaTY - JOUR AU - Elaković, Ivana AU - Đorđević, Ana D. AU - Adžić, Miroslav AU - Đorđević, Jelena D. AU - Radojčić, Marija AU - Matić, Gordana PY - 2011 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4277 AB - Gender-related differences in dexamethasone binding to corticosteroid receptors (CR) and in glucocorticoid receptor (GR) protein level in the pituitary, hypothalamus, hippocampus and prefrontal cortex were studied before and after antidepressant fluoxetine administration to both unstressed and rats exposed to a chronic social isolation stress. Untreated males, in comparison to females, displayed higher hormone-binding capacity of both GR and mineralocorticoid receptor (MR) in the hippocampal cytosol, as well as higher GR protein level in the pituitary cytosol. In both genders, dexamethasone binding to MR exceeded that to GR. While fluoxetine treatment and social isolation had no effect on GR activity, the influence on MR was gender-specific. Fluoxetine facilitated MR hormone-binding only in females, increasing the MR/GA activity ratio. In contrast, after a 6-week isolation of males, MR binding capacity was diminished and MR/GR ratio inverted in favor of GR In addition, fluoxetine induced elevation of cytosolic GR protein level in the pituitary and hypothalamus, the latter change being gender-specific. The results point to gender-related differences in the CRs functioning and suggest that both MR and GR may contribute to well-known sexual dimorphism in vulnerability to stress and stress-related disorders and in the outcome of antidepressant treatment. (C) 2011 Elsevier B.V. All rights reserved. T2 - Brain Research T1 - Gender-specific response of brain corticosteroid receptors to stress and fluoxetine VL - 1384 SP - 61 EP - 68 DO - 10.1016/j.brainres.2011.01.078 ER -
@article{ author = "Elaković, Ivana and Đorđević, Ana D. and Adžić, Miroslav and Đorđević, Jelena D. and Radojčić, Marija and Matić, Gordana", year = "2011", abstract = "Gender-related differences in dexamethasone binding to corticosteroid receptors (CR) and in glucocorticoid receptor (GR) protein level in the pituitary, hypothalamus, hippocampus and prefrontal cortex were studied before and after antidepressant fluoxetine administration to both unstressed and rats exposed to a chronic social isolation stress. Untreated males, in comparison to females, displayed higher hormone-binding capacity of both GR and mineralocorticoid receptor (MR) in the hippocampal cytosol, as well as higher GR protein level in the pituitary cytosol. In both genders, dexamethasone binding to MR exceeded that to GR. While fluoxetine treatment and social isolation had no effect on GR activity, the influence on MR was gender-specific. Fluoxetine facilitated MR hormone-binding only in females, increasing the MR/GA activity ratio. In contrast, after a 6-week isolation of males, MR binding capacity was diminished and MR/GR ratio inverted in favor of GR In addition, fluoxetine induced elevation of cytosolic GR protein level in the pituitary and hypothalamus, the latter change being gender-specific. The results point to gender-related differences in the CRs functioning and suggest that both MR and GR may contribute to well-known sexual dimorphism in vulnerability to stress and stress-related disorders and in the outcome of antidepressant treatment. (C) 2011 Elsevier B.V. All rights reserved.", journal = "Brain Research", title = "Gender-specific response of brain corticosteroid receptors to stress and fluoxetine", volume = "1384", pages = "61-68", doi = "10.1016/j.brainres.2011.01.078" }
Elaković, I., Đorđević, A. D., Adžić, M., Đorđević, J. D., Radojčić, M.,& Matić, G.. (2011). Gender-specific response of brain corticosteroid receptors to stress and fluoxetine. in Brain Research, 1384, 61-68. https://doi.org/10.1016/j.brainres.2011.01.078
Elaković I, Đorđević AD, Adžić M, Đorđević JD, Radojčić M, Matić G. Gender-specific response of brain corticosteroid receptors to stress and fluoxetine. in Brain Research. 2011;1384:61-68. doi:10.1016/j.brainres.2011.01.078 .
Elaković, Ivana, Đorđević, Ana D., Adžić, Miroslav, Đorđević, Jelena D., Radojčić, Marija, Matić, Gordana, "Gender-specific response of brain corticosteroid receptors to stress and fluoxetine" in Brain Research, 1384 (2011):61-68, https://doi.org/10.1016/j.brainres.2011.01.078 . .