Levels of Estrogen Receptor B Splice Variant (Erb Delta 5) Mrna Correlates with Progesterone Receptor in Breast Carcinomas
2010
Autori
Mandušić, VesnaPopov-Celeketic, Dusan
Nešković-Konstantinović, Zora
Kanjer, Ksenija
Božović, Ana M.
Nikolić-Vukosavljević, Dragica
Članak u časopisu
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
It is well known that breast tumors which are estrogen positive ER(+) are more likely to respond to hormone therapy. However, a certain percentage of ER(+)/PR(+) tumors do not respond to this therapy. Identification of the second estrogen receptor, named estrogen receptor beta (ER), as well as the existence of numerous isoforms/splice variants of both ER alpha and ER beta, suggests that a complex regulation of estrogen action exists. In this study, we analyzed the expression ratio of ER beta 1 isoform and ER beta Delta 5 splice variant mRNAs, and its correlation with ER/PR status by quantitative RT-PCR and clinical and histopathological parameters. We found that the relative proportion of ER beta Delta 5 in the total ER beta 1 transcript pool inversely correlates with the PR level (rho = -0,359, p LT 0,003, Spearman). It may be that the ER beta Delta 5 variant modulates the ERa activity of downstream targets. In addition, we suggest that the determination of the expression profiles of ...ER alpha and ER beta isoforms and splice variants in the defined groups of patients are necessary for elucidating their involvement in endocrine resistance.
Ključne reči:
Estrogen receptor beta / progesterone receptor / quantitative RT-PCR / breast cancer / normalization of splice variant to wt isoformIzvor:
Archives of Biological Sciences, 2010, 62, 2, 257-262Finansiranje / projekti:
- Serbian Ministry of Science and Technological development [143010, 145018]
DOI: 10.2298/ABS1002257M
ISSN: 0354-4664
WoS: 000280017400007
Scopus: 2-s2.0-77955153781
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Mandušić, Vesna AU - Popov-Celeketic, Dusan AU - Nešković-Konstantinović, Zora AU - Kanjer, Ksenija AU - Božović, Ana M. AU - Nikolić-Vukosavljević, Dragica PY - 2010 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4046 AB - It is well known that breast tumors which are estrogen positive ER(+) are more likely to respond to hormone therapy. However, a certain percentage of ER(+)/PR(+) tumors do not respond to this therapy. Identification of the second estrogen receptor, named estrogen receptor beta (ER), as well as the existence of numerous isoforms/splice variants of both ER alpha and ER beta, suggests that a complex regulation of estrogen action exists. In this study, we analyzed the expression ratio of ER beta 1 isoform and ER beta Delta 5 splice variant mRNAs, and its correlation with ER/PR status by quantitative RT-PCR and clinical and histopathological parameters. We found that the relative proportion of ER beta Delta 5 in the total ER beta 1 transcript pool inversely correlates with the PR level (rho = -0,359, p LT 0,003, Spearman). It may be that the ER beta Delta 5 variant modulates the ERa activity of downstream targets. In addition, we suggest that the determination of the expression profiles of ER alpha and ER beta isoforms and splice variants in the defined groups of patients are necessary for elucidating their involvement in endocrine resistance. T2 - Archives of Biological Sciences T1 - Levels of Estrogen Receptor B Splice Variant (Erb Delta 5) Mrna Correlates with Progesterone Receptor in Breast Carcinomas VL - 62 IS - 2 SP - 257 EP - 262 DO - 10.2298/ABS1002257M ER -
@article{ author = "Mandušić, Vesna and Popov-Celeketic, Dusan and Nešković-Konstantinović, Zora and Kanjer, Ksenija and Božović, Ana M. and Nikolić-Vukosavljević, Dragica", year = "2010", abstract = "It is well known that breast tumors which are estrogen positive ER(+) are more likely to respond to hormone therapy. However, a certain percentage of ER(+)/PR(+) tumors do not respond to this therapy. Identification of the second estrogen receptor, named estrogen receptor beta (ER), as well as the existence of numerous isoforms/splice variants of both ER alpha and ER beta, suggests that a complex regulation of estrogen action exists. In this study, we analyzed the expression ratio of ER beta 1 isoform and ER beta Delta 5 splice variant mRNAs, and its correlation with ER/PR status by quantitative RT-PCR and clinical and histopathological parameters. We found that the relative proportion of ER beta Delta 5 in the total ER beta 1 transcript pool inversely correlates with the PR level (rho = -0,359, p LT 0,003, Spearman). It may be that the ER beta Delta 5 variant modulates the ERa activity of downstream targets. In addition, we suggest that the determination of the expression profiles of ER alpha and ER beta isoforms and splice variants in the defined groups of patients are necessary for elucidating their involvement in endocrine resistance.", journal = "Archives of Biological Sciences", title = "Levels of Estrogen Receptor B Splice Variant (Erb Delta 5) Mrna Correlates with Progesterone Receptor in Breast Carcinomas", volume = "62", number = "2", pages = "257-262", doi = "10.2298/ABS1002257M" }
Mandušić, V., Popov-Celeketic, D., Nešković-Konstantinović, Z., Kanjer, K., Božović, A. M.,& Nikolić-Vukosavljević, D.. (2010). Levels of Estrogen Receptor B Splice Variant (Erb Delta 5) Mrna Correlates with Progesterone Receptor in Breast Carcinomas. in Archives of Biological Sciences, 62(2), 257-262. https://doi.org/10.2298/ABS1002257M
Mandušić V, Popov-Celeketic D, Nešković-Konstantinović Z, Kanjer K, Božović AM, Nikolić-Vukosavljević D. Levels of Estrogen Receptor B Splice Variant (Erb Delta 5) Mrna Correlates with Progesterone Receptor in Breast Carcinomas. in Archives of Biological Sciences. 2010;62(2):257-262. doi:10.2298/ABS1002257M .
Mandušić, Vesna, Popov-Celeketic, Dusan, Nešković-Konstantinović, Zora, Kanjer, Ksenija, Božović, Ana M., Nikolić-Vukosavljević, Dragica, "Levels of Estrogen Receptor B Splice Variant (Erb Delta 5) Mrna Correlates with Progesterone Receptor in Breast Carcinomas" in Archives of Biological Sciences, 62, no. 2 (2010):257-262, https://doi.org/10.2298/ABS1002257M . .