Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species
Нема приказа
Аутори
Aljancic, IvanaStanković, Miroslava
Tešević, Vele V.
Vujisić, Ljubodrag
Vajs, Vlatka
Milosavljević, Slobodan
Чланак у часопису
Метаподаци
Приказ свих података о документуАпстракт
This study was conducted to elucidate the in vitro protective effect of five flavonoids [apigenin (1), apigenin-7-O-glucoside (2), centaureidin (3), jaceidin (4) and quercetin (5)] against chromosomal damage in mitogen-induced human lymphocytes. Using the Cytochalasin-B blocked micronucleus (CBMN) assay, in which the biomarker of chromosome breakage and/or chromosome loss is the elevated frequency of micronucleus (MN) in binucleated (BN) cells, the presence of flavonoid 2 in minimal concentration (3 mu g/mL) gave a 35.5% decrease M the frequency of MN when compared with control human lymphocytes. The same concentration of flavonoids 1, 3 and 4, reduced the MN frequency by 24.4%, 28.0% and 28.0%, respectively. Higher concentrations (6 mu g/mL and 10 mu g/mL) seemed less effective. Flavonoid 5 (3 mu g/mL.) induced a slight decrease in MN frequency (5%), while higher doses (6 mu g/mL and 10 mu g/mL) provoked an increase of DNA damage. The comparable values for the cytokinesis-block prolif...eration index (CBPI) of the tested flavonoids and positive control suggested an inhibitory effect on lymphocyte proliferation. In the DPPH scavenging assay, flavonoids 1-4 demonstrated modest activity, in a dose-dependent manner, compared with the synthetic antioxidants BHT and Trolox, while 5 exhibited comparably high antioxidative activity.
Кључне речи:
Flavonoids / CBMN / DPPH / BHTИзвор:
Natural Product Communications, 2010, 5, 5, 729-732Финансирање / пројекти:
- Ministry of Sciences and Technological Development of the Republic of Serbia [142053]
Колекције
Институција/група
VinčaTY - JOUR AU - Aljancic, Ivana AU - Stanković, Miroslava AU - Tešević, Vele V. AU - Vujisić, Ljubodrag AU - Vajs, Vlatka AU - Milosavljević, Slobodan PY - 2010 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4002 AB - This study was conducted to elucidate the in vitro protective effect of five flavonoids [apigenin (1), apigenin-7-O-glucoside (2), centaureidin (3), jaceidin (4) and quercetin (5)] against chromosomal damage in mitogen-induced human lymphocytes. Using the Cytochalasin-B blocked micronucleus (CBMN) assay, in which the biomarker of chromosome breakage and/or chromosome loss is the elevated frequency of micronucleus (MN) in binucleated (BN) cells, the presence of flavonoid 2 in minimal concentration (3 mu g/mL) gave a 35.5% decrease M the frequency of MN when compared with control human lymphocytes. The same concentration of flavonoids 1, 3 and 4, reduced the MN frequency by 24.4%, 28.0% and 28.0%, respectively. Higher concentrations (6 mu g/mL and 10 mu g/mL) seemed less effective. Flavonoid 5 (3 mu g/mL.) induced a slight decrease in MN frequency (5%), while higher doses (6 mu g/mL and 10 mu g/mL) provoked an increase of DNA damage. The comparable values for the cytokinesis-block proliferation index (CBPI) of the tested flavonoids and positive control suggested an inhibitory effect on lymphocyte proliferation. In the DPPH scavenging assay, flavonoids 1-4 demonstrated modest activity, in a dose-dependent manner, compared with the synthetic antioxidants BHT and Trolox, while 5 exhibited comparably high antioxidative activity. T2 - Natural Product Communications T1 - Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species VL - 5 IS - 5 SP - 729 EP - 732 UR - https://hdl.handle.net/21.15107/rcub_vinar_4002 ER -
@article{ author = "Aljancic, Ivana and Stanković, Miroslava and Tešević, Vele V. and Vujisić, Ljubodrag and Vajs, Vlatka and Milosavljević, Slobodan", year = "2010", abstract = "This study was conducted to elucidate the in vitro protective effect of five flavonoids [apigenin (1), apigenin-7-O-glucoside (2), centaureidin (3), jaceidin (4) and quercetin (5)] against chromosomal damage in mitogen-induced human lymphocytes. Using the Cytochalasin-B blocked micronucleus (CBMN) assay, in which the biomarker of chromosome breakage and/or chromosome loss is the elevated frequency of micronucleus (MN) in binucleated (BN) cells, the presence of flavonoid 2 in minimal concentration (3 mu g/mL) gave a 35.5% decrease M the frequency of MN when compared with control human lymphocytes. The same concentration of flavonoids 1, 3 and 4, reduced the MN frequency by 24.4%, 28.0% and 28.0%, respectively. Higher concentrations (6 mu g/mL and 10 mu g/mL) seemed less effective. Flavonoid 5 (3 mu g/mL.) induced a slight decrease in MN frequency (5%), while higher doses (6 mu g/mL and 10 mu g/mL) provoked an increase of DNA damage. The comparable values for the cytokinesis-block proliferation index (CBPI) of the tested flavonoids and positive control suggested an inhibitory effect on lymphocyte proliferation. In the DPPH scavenging assay, flavonoids 1-4 demonstrated modest activity, in a dose-dependent manner, compared with the synthetic antioxidants BHT and Trolox, while 5 exhibited comparably high antioxidative activity.", journal = "Natural Product Communications", title = "Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species", volume = "5", number = "5", pages = "729-732", url = "https://hdl.handle.net/21.15107/rcub_vinar_4002" }
Aljancic, I., Stanković, M., Tešević, V. V., Vujisić, L., Vajs, V.,& Milosavljević, S.. (2010). Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species. in Natural Product Communications, 5(5), 729-732. https://hdl.handle.net/21.15107/rcub_vinar_4002
Aljancic I, Stanković M, Tešević VV, Vujisić L, Vajs V, Milosavljević S. Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species. in Natural Product Communications. 2010;5(5):729-732. https://hdl.handle.net/21.15107/rcub_vinar_4002 .
Aljancic, Ivana, Stanković, Miroslava, Tešević, Vele V., Vujisić, Ljubodrag, Vajs, Vlatka, Milosavljević, Slobodan, "Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species" in Natural Product Communications, 5, no. 5 (2010):729-732, https://hdl.handle.net/21.15107/rcub_vinar_4002 .