Properties of grape seed Proanthocyanidins and quercetin in human lymphocytes
Abstract
This study addresses the in vitro protective effects of proanthocyanidins and quercetin on the frequency of micronuclei with reference to the antioxidant status of cultured human lymphocytes also subjected to 2 Gy of gamma-radiation. Treatment of lymphocytes with proanthocyanidins induced a significant decrease in the frequency of micronuclei and reduction of malonyldialdehyde production, as well as increased catalase and glutathione S-transferase activity Quercetin induces a higher incidence of micronuclei and production of malonyldialdehyde. The seven-fold increase of micronuclei frequency induced by a therapeutic dose of gamma-radiation was decreased in the presence of proanthocyanidins. These results demonstrate that proanthocyanidins may be important in the prevention of ROS-induced oxidative lymphocyte damage by decreasing DNA damage, lowering membrane lipid peroxidation, and increasing the activity AOP enzymes, as well as by reducing the level of gamma-radiation-induced DNA dama...ge. Our results support the potential benefits of proanthocyanidins as efficient antioxidants and radioprotectors.
Keywords:
micronucleus test / flavonoids / malonyldialdehyde / catalase / glutathione S-transferase / gamma-radiation / human lymphocytesSource:
Archives of Biological Sciences, 2008, 60, 3, 367-377Funding / projects:
- Signalni putevi delovanja steroidnih hormona i uticaj endogenih i egzogenih faktora na modulaciju procesa u ćelijama sisara (RS-MESTD-MPN2006-2010-143044)
DOI: 10.2298/ABS0803367S
ISSN: 0354-4664; 1821-4339
WoS: 000258524700005
Scopus: 2-s2.0-53549096234
Collections
Institution/Community
VinčaTY - JOUR AU - Stanković, Miroslava AU - Horvat, Anica PY - 2008 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3505 AB - This study addresses the in vitro protective effects of proanthocyanidins and quercetin on the frequency of micronuclei with reference to the antioxidant status of cultured human lymphocytes also subjected to 2 Gy of gamma-radiation. Treatment of lymphocytes with proanthocyanidins induced a significant decrease in the frequency of micronuclei and reduction of malonyldialdehyde production, as well as increased catalase and glutathione S-transferase activity Quercetin induces a higher incidence of micronuclei and production of malonyldialdehyde. The seven-fold increase of micronuclei frequency induced by a therapeutic dose of gamma-radiation was decreased in the presence of proanthocyanidins. These results demonstrate that proanthocyanidins may be important in the prevention of ROS-induced oxidative lymphocyte damage by decreasing DNA damage, lowering membrane lipid peroxidation, and increasing the activity AOP enzymes, as well as by reducing the level of gamma-radiation-induced DNA damage. Our results support the potential benefits of proanthocyanidins as efficient antioxidants and radioprotectors. T2 - Archives of Biological Sciences T1 - Properties of grape seed Proanthocyanidins and quercetin in human lymphocytes VL - 60 IS - 3 SP - 367 EP - 377 DO - 10.2298/ABS0803367S ER -
@article{ author = "Stanković, Miroslava and Horvat, Anica", year = "2008", abstract = "This study addresses the in vitro protective effects of proanthocyanidins and quercetin on the frequency of micronuclei with reference to the antioxidant status of cultured human lymphocytes also subjected to 2 Gy of gamma-radiation. Treatment of lymphocytes with proanthocyanidins induced a significant decrease in the frequency of micronuclei and reduction of malonyldialdehyde production, as well as increased catalase and glutathione S-transferase activity Quercetin induces a higher incidence of micronuclei and production of malonyldialdehyde. The seven-fold increase of micronuclei frequency induced by a therapeutic dose of gamma-radiation was decreased in the presence of proanthocyanidins. These results demonstrate that proanthocyanidins may be important in the prevention of ROS-induced oxidative lymphocyte damage by decreasing DNA damage, lowering membrane lipid peroxidation, and increasing the activity AOP enzymes, as well as by reducing the level of gamma-radiation-induced DNA damage. Our results support the potential benefits of proanthocyanidins as efficient antioxidants and radioprotectors.", journal = "Archives of Biological Sciences", title = "Properties of grape seed Proanthocyanidins and quercetin in human lymphocytes", volume = "60", number = "3", pages = "367-377", doi = "10.2298/ABS0803367S" }
Stanković, M.,& Horvat, A.. (2008). Properties of grape seed Proanthocyanidins and quercetin in human lymphocytes. in Archives of Biological Sciences, 60(3), 367-377. https://doi.org/10.2298/ABS0803367S
Stanković M, Horvat A. Properties of grape seed Proanthocyanidins and quercetin in human lymphocytes. in Archives of Biological Sciences. 2008;60(3):367-377. doi:10.2298/ABS0803367S .
Stanković, Miroslava, Horvat, Anica, "Properties of grape seed Proanthocyanidins and quercetin in human lymphocytes" in Archives of Biological Sciences, 60, no. 3 (2008):367-377, https://doi.org/10.2298/ABS0803367S . .