Inhibition of AChE by single and simultaneous exposure to malathion and its degradation products
Нема приказа
Аутори
Krstić, Danijela Z.Čolović, Mirjana B.
Krinulović, Katarina
Đurić, Dragan M.
Vasić, Vesna M.
Чланак у часопису
Метаподаци
Приказ свих података о документуАпстракт
In vitro inhibition of bovine erythrocytes acetylcholinesterase (AchE) by separate and simultaneous exposure to organophosphorous insecticide malathion and the transformation products, which are generally formed during the storage or natural as well as photochemical degradation pathways of malathion, was investigated. The increasing concentration of malathion, its oxidation product - malaoxon and isomerisation product - isomalathion inhibited AChE activity in a concentration-dependent manner. The half-maximum inhibitory concentrations (IC50 values): (3.2 +/- 0.1) x 10(-5) mol/l, (4.7 +/- 0.8) x 10(-7) mol/l and (6.0 +/- 0.5) x 10(-7) mol/l were obtained from the inhibition curves induced by malathion, malaoxon and isomalathion, respectively. However, the products formed due to photoinduced degradation, phosphorodithioic O,O,S-trimethyl phosphorodithioic ester (OOS(S)) and O,O-dimethyl thiophosphate did not noticeably affect the enzyme activity at all investigated concentrations, while ...diethyl maleate inhibited the AChE activity at concentrations GT 10 mmol/l. By simultaneous exposure of the enzyme to malaoxon and isomalathion in various concentration combinations the additive effect was achieved by low concentration of inhibitors, while the antagonistic effect was obtained at high concentration ( GT = 3 x 10-7 mol/l) of inhibitors. Inhibitory power of irradiated samples of 1 x 10(-5) mol/l malathion can be attributed to the formation of malaoxon and isomalathion, organophosphates about 100 times more toxic than their parent compound, while the presence of non-inhibiting degradation product OOS(S) did not affect the inhibitor efficiency of inhibiting malathion by-products, malaoxon and isomalathion.
Кључне речи:
acetylcholinesterase / malathion degradation productsИзвор:
General Physiology and Biophysics, 2007, 26, 4, 247-253Колекције
Институција/група
VinčaTY - JOUR AU - Krstić, Danijela Z. AU - Čolović, Mirjana B. AU - Krinulović, Katarina AU - Đurić, Dragan M. AU - Vasić, Vesna M. PY - 2007 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3364 AB - In vitro inhibition of bovine erythrocytes acetylcholinesterase (AchE) by separate and simultaneous exposure to organophosphorous insecticide malathion and the transformation products, which are generally formed during the storage or natural as well as photochemical degradation pathways of malathion, was investigated. The increasing concentration of malathion, its oxidation product - malaoxon and isomerisation product - isomalathion inhibited AChE activity in a concentration-dependent manner. The half-maximum inhibitory concentrations (IC50 values): (3.2 +/- 0.1) x 10(-5) mol/l, (4.7 +/- 0.8) x 10(-7) mol/l and (6.0 +/- 0.5) x 10(-7) mol/l were obtained from the inhibition curves induced by malathion, malaoxon and isomalathion, respectively. However, the products formed due to photoinduced degradation, phosphorodithioic O,O,S-trimethyl phosphorodithioic ester (OOS(S)) and O,O-dimethyl thiophosphate did not noticeably affect the enzyme activity at all investigated concentrations, while diethyl maleate inhibited the AChE activity at concentrations GT 10 mmol/l. By simultaneous exposure of the enzyme to malaoxon and isomalathion in various concentration combinations the additive effect was achieved by low concentration of inhibitors, while the antagonistic effect was obtained at high concentration ( GT = 3 x 10-7 mol/l) of inhibitors. Inhibitory power of irradiated samples of 1 x 10(-5) mol/l malathion can be attributed to the formation of malaoxon and isomalathion, organophosphates about 100 times more toxic than their parent compound, while the presence of non-inhibiting degradation product OOS(S) did not affect the inhibitor efficiency of inhibiting malathion by-products, malaoxon and isomalathion. T2 - General Physiology and Biophysics T1 - Inhibition of AChE by single and simultaneous exposure to malathion and its degradation products VL - 26 IS - 4 SP - 247 EP - 253 UR - https://hdl.handle.net/21.15107/rcub_vinar_3364 ER -
@article{ author = "Krstić, Danijela Z. and Čolović, Mirjana B. and Krinulović, Katarina and Đurić, Dragan M. and Vasić, Vesna M.", year = "2007", abstract = "In vitro inhibition of bovine erythrocytes acetylcholinesterase (AchE) by separate and simultaneous exposure to organophosphorous insecticide malathion and the transformation products, which are generally formed during the storage or natural as well as photochemical degradation pathways of malathion, was investigated. The increasing concentration of malathion, its oxidation product - malaoxon and isomerisation product - isomalathion inhibited AChE activity in a concentration-dependent manner. The half-maximum inhibitory concentrations (IC50 values): (3.2 +/- 0.1) x 10(-5) mol/l, (4.7 +/- 0.8) x 10(-7) mol/l and (6.0 +/- 0.5) x 10(-7) mol/l were obtained from the inhibition curves induced by malathion, malaoxon and isomalathion, respectively. However, the products formed due to photoinduced degradation, phosphorodithioic O,O,S-trimethyl phosphorodithioic ester (OOS(S)) and O,O-dimethyl thiophosphate did not noticeably affect the enzyme activity at all investigated concentrations, while diethyl maleate inhibited the AChE activity at concentrations GT 10 mmol/l. By simultaneous exposure of the enzyme to malaoxon and isomalathion in various concentration combinations the additive effect was achieved by low concentration of inhibitors, while the antagonistic effect was obtained at high concentration ( GT = 3 x 10-7 mol/l) of inhibitors. Inhibitory power of irradiated samples of 1 x 10(-5) mol/l malathion can be attributed to the formation of malaoxon and isomalathion, organophosphates about 100 times more toxic than their parent compound, while the presence of non-inhibiting degradation product OOS(S) did not affect the inhibitor efficiency of inhibiting malathion by-products, malaoxon and isomalathion.", journal = "General Physiology and Biophysics", title = "Inhibition of AChE by single and simultaneous exposure to malathion and its degradation products", volume = "26", number = "4", pages = "247-253", url = "https://hdl.handle.net/21.15107/rcub_vinar_3364" }
Krstić, D. Z., Čolović, M. B., Krinulović, K., Đurić, D. M.,& Vasić, V. M.. (2007). Inhibition of AChE by single and simultaneous exposure to malathion and its degradation products. in General Physiology and Biophysics, 26(4), 247-253. https://hdl.handle.net/21.15107/rcub_vinar_3364
Krstić DZ, Čolović MB, Krinulović K, Đurić DM, Vasić VM. Inhibition of AChE by single and simultaneous exposure to malathion and its degradation products. in General Physiology and Biophysics. 2007;26(4):247-253. https://hdl.handle.net/21.15107/rcub_vinar_3364 .
Krstić, Danijela Z., Čolović, Mirjana B., Krinulović, Katarina, Đurić, Dragan M., Vasić, Vesna M., "Inhibition of AChE by single and simultaneous exposure to malathion and its degradation products" in General Physiology and Biophysics, 26, no. 4 (2007):247-253, https://hdl.handle.net/21.15107/rcub_vinar_3364 .