Supported liquid membrane extraction of Lu-177(III) with DEHPA and its application for purification of Lu-177-DOTA-lanreotide
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Kumrić, KsenijaTrtić-Petrović, Tatjana M.
Koumarianou, Eftichia
Archimandritis, Spyridon
Čomor, Jožef J.
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Permeation of Lu-177(III) through supported hexane membrane containing di-(2-ethythexyl)phosphoric acid (DEHPA) as a carrier has been studied. The donor solution was 0.2 mol dm(-3) ammonium acetate buffer, pH 5.0-5.5, that is optimal for peptide labeling, and the acceptor solution was 2 mol dm(-3) HCl. A miniaturized supported liquid membrane (SLM) contactor with 10 mu l donor and acceptor channel and ultra thin, flat poly(tetrafluoroethene) laminated on polyester fleece (PTFE) was applied in the work reported herein. The donor phase was pumped continuously through the donor channel, while the acceptor was stagnant during the SLM extraction. The influences of carrier concentration and donor flow rate on extraction of Lu-177(III) have been investigated. The results are discussed in the term of mass transfer coefficient, mass transfer resistance and extraction efficiency. Based on the experimental results of SLM extraction of Lu-177(III), this technique has been applied for the separatio...n of free radionuclide from the labeled peptide as a purification procedure within the production of the radiopharmaceutical. An analog of somatostatin, lanreotide, which was conjugated with chelating moiety 1,4,7,10-tetraazacyclododecane-N,N,N,N-tetraacetic acid (DOTA) was labeled with Lu-177(III). The characterization of the labeled conjugate as well as the complexation yield and the efficiency of purification by membrane extraction were carried out by HPLC analysis. (c) 2006 Elsevier B.V. All rights reserved.
Keywords:
Lu-177 / peptide / lanreotide / supported liquid membrane extraction (SLM) / Di(2-ethylhexyl)phosphoric acidSource:
Separation and Purification Technology, 2006, 51, 3, 310-317
DOI: 10.1016/j.seppur.2006.02.011
ISSN: 1383-5866
WoS: 000241652500011
Scopus: 2-s2.0-33749070382
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VinčaTY - JOUR AU - Kumrić, Ksenija AU - Trtić-Petrović, Tatjana M. AU - Koumarianou, Eftichia AU - Archimandritis, Spyridon AU - Čomor, Jožef J. PY - 2006 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3111 AB - Permeation of Lu-177(III) through supported hexane membrane containing di-(2-ethythexyl)phosphoric acid (DEHPA) as a carrier has been studied. The donor solution was 0.2 mol dm(-3) ammonium acetate buffer, pH 5.0-5.5, that is optimal for peptide labeling, and the acceptor solution was 2 mol dm(-3) HCl. A miniaturized supported liquid membrane (SLM) contactor with 10 mu l donor and acceptor channel and ultra thin, flat poly(tetrafluoroethene) laminated on polyester fleece (PTFE) was applied in the work reported herein. The donor phase was pumped continuously through the donor channel, while the acceptor was stagnant during the SLM extraction. The influences of carrier concentration and donor flow rate on extraction of Lu-177(III) have been investigated. The results are discussed in the term of mass transfer coefficient, mass transfer resistance and extraction efficiency. Based on the experimental results of SLM extraction of Lu-177(III), this technique has been applied for the separation of free radionuclide from the labeled peptide as a purification procedure within the production of the radiopharmaceutical. An analog of somatostatin, lanreotide, which was conjugated with chelating moiety 1,4,7,10-tetraazacyclododecane-N,N,N,N-tetraacetic acid (DOTA) was labeled with Lu-177(III). The characterization of the labeled conjugate as well as the complexation yield and the efficiency of purification by membrane extraction were carried out by HPLC analysis. (c) 2006 Elsevier B.V. All rights reserved. T2 - Separation and Purification Technology T1 - Supported liquid membrane extraction of Lu-177(III) with DEHPA and its application for purification of Lu-177-DOTA-lanreotide VL - 51 IS - 3 SP - 310 EP - 317 DO - 10.1016/j.seppur.2006.02.011 ER -
@article{ author = "Kumrić, Ksenija and Trtić-Petrović, Tatjana M. and Koumarianou, Eftichia and Archimandritis, Spyridon and Čomor, Jožef J.", year = "2006", abstract = "Permeation of Lu-177(III) through supported hexane membrane containing di-(2-ethythexyl)phosphoric acid (DEHPA) as a carrier has been studied. The donor solution was 0.2 mol dm(-3) ammonium acetate buffer, pH 5.0-5.5, that is optimal for peptide labeling, and the acceptor solution was 2 mol dm(-3) HCl. A miniaturized supported liquid membrane (SLM) contactor with 10 mu l donor and acceptor channel and ultra thin, flat poly(tetrafluoroethene) laminated on polyester fleece (PTFE) was applied in the work reported herein. The donor phase was pumped continuously through the donor channel, while the acceptor was stagnant during the SLM extraction. The influences of carrier concentration and donor flow rate on extraction of Lu-177(III) have been investigated. The results are discussed in the term of mass transfer coefficient, mass transfer resistance and extraction efficiency. Based on the experimental results of SLM extraction of Lu-177(III), this technique has been applied for the separation of free radionuclide from the labeled peptide as a purification procedure within the production of the radiopharmaceutical. An analog of somatostatin, lanreotide, which was conjugated with chelating moiety 1,4,7,10-tetraazacyclododecane-N,N,N,N-tetraacetic acid (DOTA) was labeled with Lu-177(III). The characterization of the labeled conjugate as well as the complexation yield and the efficiency of purification by membrane extraction were carried out by HPLC analysis. (c) 2006 Elsevier B.V. All rights reserved.", journal = "Separation and Purification Technology", title = "Supported liquid membrane extraction of Lu-177(III) with DEHPA and its application for purification of Lu-177-DOTA-lanreotide", volume = "51", number = "3", pages = "310-317", doi = "10.1016/j.seppur.2006.02.011" }
Kumrić, K., Trtić-Petrović, T. M., Koumarianou, E., Archimandritis, S.,& Čomor, J. J.. (2006). Supported liquid membrane extraction of Lu-177(III) with DEHPA and its application for purification of Lu-177-DOTA-lanreotide. in Separation and Purification Technology, 51(3), 310-317. https://doi.org/10.1016/j.seppur.2006.02.011
Kumrić K, Trtić-Petrović TM, Koumarianou E, Archimandritis S, Čomor JJ. Supported liquid membrane extraction of Lu-177(III) with DEHPA and its application for purification of Lu-177-DOTA-lanreotide. in Separation and Purification Technology. 2006;51(3):310-317. doi:10.1016/j.seppur.2006.02.011 .
Kumrić, Ksenija, Trtić-Petrović, Tatjana M., Koumarianou, Eftichia, Archimandritis, Spyridon, Čomor, Jožef J., "Supported liquid membrane extraction of Lu-177(III) with DEHPA and its application for purification of Lu-177-DOTA-lanreotide" in Separation and Purification Technology, 51, no. 3 (2006):310-317, https://doi.org/10.1016/j.seppur.2006.02.011 . .