Kinetics of thyroxine (T-4) and triiodothyronine (T-3) transport in the isolated rat heart
2001
Authors
Rosić, Mirko A.Pantović, Suzana B.
Lučić, Aleksandra P.
Ribarac-Stepić, Nevena B.
Anđelković, Ivan Z.
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The dynamics and kinetics of thyroid hormone transport in the isolated rat heart were examined using the modified unidirectional paired tracer dilution method. The uptake of I-125-thyroxine (I-115-T-4) and I-125-triiodothyronine (I-125-T-3) from the extracellular space into heart cells was measured relative to the extracellular space marker H-3-mannitol. The thyroid hormone maximal uptake was 54.4 % for I-125-T-4 and 52.15 % for I-125-T-3. The thyroid hormone net uptake was 25.69 % for I-125-T-4 and 25.49 % for I-125-T-3. Backflux from the intracellular space was 53.17 % for I-125-T-4 and 61.59 % for I-125-T-3. In the presence of unlabelled thyroid hormones, I-125-T-4 and I-125-T-3 maximal uptakes were reduced from 10.1 to 59.74 % and from 34.6 to 65.3 %, respectively, depending on the concentration of the unlabelled hormone, suggesting a saturable mechanism of the thyroid hormone uptake by the heart cells, with K-m(T4) = 105.46 muM and the maximal rate of I-125-thyroid hormone flax fr...om the extracellular space to heart cells (V-max(T4))= 177.84 nM min(-1) for I-125-T-4 uptake, and K-m(T3) = 80.0 muM and V-max(T3) = 118.5 nM min(-1) for I-125-T-3 uptake.
Source:
Experimental Physiology, 2001, 86, 1, 13-18
DOI: 10.1113/eph8601835
ISSN: 0958-0670
PubMed: 11429614
WoS: 000166914100002
Scopus: 2-s2.0-0035135522
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VinčaTY - JOUR AU - Rosić, Mirko A. AU - Pantović, Suzana B. AU - Lučić, Aleksandra P. AU - Ribarac-Stepić, Nevena B. AU - Anđelković, Ivan Z. PY - 2001 UR - https://vinar.vin.bg.ac.rs/handle/123456789/2413 AB - The dynamics and kinetics of thyroid hormone transport in the isolated rat heart were examined using the modified unidirectional paired tracer dilution method. The uptake of I-125-thyroxine (I-115-T-4) and I-125-triiodothyronine (I-125-T-3) from the extracellular space into heart cells was measured relative to the extracellular space marker H-3-mannitol. The thyroid hormone maximal uptake was 54.4 % for I-125-T-4 and 52.15 % for I-125-T-3. The thyroid hormone net uptake was 25.69 % for I-125-T-4 and 25.49 % for I-125-T-3. Backflux from the intracellular space was 53.17 % for I-125-T-4 and 61.59 % for I-125-T-3. In the presence of unlabelled thyroid hormones, I-125-T-4 and I-125-T-3 maximal uptakes were reduced from 10.1 to 59.74 % and from 34.6 to 65.3 %, respectively, depending on the concentration of the unlabelled hormone, suggesting a saturable mechanism of the thyroid hormone uptake by the heart cells, with K-m(T4) = 105.46 muM and the maximal rate of I-125-thyroid hormone flax from the extracellular space to heart cells (V-max(T4))= 177.84 nM min(-1) for I-125-T-4 uptake, and K-m(T3) = 80.0 muM and V-max(T3) = 118.5 nM min(-1) for I-125-T-3 uptake. T2 - Experimental Physiology T1 - Kinetics of thyroxine (T-4) and triiodothyronine (T-3) transport in the isolated rat heart VL - 86 IS - 1 SP - 13 EP - 18 DO - 10.1113/eph8601835 ER -
@article{ author = "Rosić, Mirko A. and Pantović, Suzana B. and Lučić, Aleksandra P. and Ribarac-Stepić, Nevena B. and Anđelković, Ivan Z.", year = "2001", abstract = "The dynamics and kinetics of thyroid hormone transport in the isolated rat heart were examined using the modified unidirectional paired tracer dilution method. The uptake of I-125-thyroxine (I-115-T-4) and I-125-triiodothyronine (I-125-T-3) from the extracellular space into heart cells was measured relative to the extracellular space marker H-3-mannitol. The thyroid hormone maximal uptake was 54.4 % for I-125-T-4 and 52.15 % for I-125-T-3. The thyroid hormone net uptake was 25.69 % for I-125-T-4 and 25.49 % for I-125-T-3. Backflux from the intracellular space was 53.17 % for I-125-T-4 and 61.59 % for I-125-T-3. In the presence of unlabelled thyroid hormones, I-125-T-4 and I-125-T-3 maximal uptakes were reduced from 10.1 to 59.74 % and from 34.6 to 65.3 %, respectively, depending on the concentration of the unlabelled hormone, suggesting a saturable mechanism of the thyroid hormone uptake by the heart cells, with K-m(T4) = 105.46 muM and the maximal rate of I-125-thyroid hormone flax from the extracellular space to heart cells (V-max(T4))= 177.84 nM min(-1) for I-125-T-4 uptake, and K-m(T3) = 80.0 muM and V-max(T3) = 118.5 nM min(-1) for I-125-T-3 uptake.", journal = "Experimental Physiology", title = "Kinetics of thyroxine (T-4) and triiodothyronine (T-3) transport in the isolated rat heart", volume = "86", number = "1", pages = "13-18", doi = "10.1113/eph8601835" }
Rosić, M. A., Pantović, S. B., Lučić, A. P., Ribarac-Stepić, N. B.,& Anđelković, I. Z.. (2001). Kinetics of thyroxine (T-4) and triiodothyronine (T-3) transport in the isolated rat heart. in Experimental Physiology, 86(1), 13-18. https://doi.org/10.1113/eph8601835
Rosić MA, Pantović SB, Lučić AP, Ribarac-Stepić NB, Anđelković IZ. Kinetics of thyroxine (T-4) and triiodothyronine (T-3) transport in the isolated rat heart. in Experimental Physiology. 2001;86(1):13-18. doi:10.1113/eph8601835 .
Rosić, Mirko A., Pantović, Suzana B., Lučić, Aleksandra P., Ribarac-Stepić, Nevena B., Anđelković, Ivan Z., "Kinetics of thyroxine (T-4) and triiodothyronine (T-3) transport in the isolated rat heart" in Experimental Physiology, 86, no. 1 (2001):13-18, https://doi.org/10.1113/eph8601835 . .