Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats
Само за регистроване кориснике
2017
Аутори
Romić, Snježana Đ.Krskova, Katarina
Olszanecki, Rafal
Balazova, Lucia
Lory, Viktoria
Korićanac, Goran
Slamkova, Miroslava
Zorad, Stefan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Fatty acid (FA) uptake and/or intramuscular triglyceride (TG) accumulation in skeletal muscle are increased in obesity, type 2 diabetes and aging. FA translocase (FAT/CD36) translocation, lipin-1 subcellular localization and nuclear factor kappa B (NF-kappa B) p65 protein content in quadriceps muscle of young and old obese Zuckerfa/fa rats and their lean controls were analyzed by immunoblot to define obesity- and aging-related alterations in FA uptake, their subsequent metabolic fate and potential to activate pro-inflammatory signaling. As expected, obesity increased FAT/CD36 content in plasma membrane in quadriceps muscle of fa/fa rats. Aging increased cytosolic lipin-1 content in both, obese rats and their lean controls. Also, old obese rats had decreased level of nuclear extract lipin-lcompared to that in old lean rats. Neither obesity nor age altered NF-kappa B p65 protein content in cytosol and nuclear extract of quadriceps muscle suggesting that obesity/aging-induced changes in F...A handling are not accompanied by NF-kappa B-mediated inflammation. Increase in plasma membrane FAT/CD36 content in obese rats and failure in lipin-1 export to nucleus with progression of obesity, implying an increase in FA uptake and their different channeling into lipid intermediates synthesis pathway in old fa/fa rats versus FA usage in lean rats of the same age.
Кључне речи:
Obesity / Aging / Skeletal muscle / FAT/CD36 / Lipin-1Извор:
General Physiology and Biophysics, 2017, 36, 4, 399-406Финансирање / пројекти:
- Улога стероидних хормона у неуроендокриној адаптацији на стрес и патофизиологији метаболичког синдрома - молекуларни механизми и клиничке импликације (RS-41009)
- SAIA National Scholarship Programme of the Slovak Republic, [VEGA 2/0174/17], [APVV-15-0229], [APVV-15-0565]
DOI: 10.4149/gpb_2017010
ISSN: 0231-5882; 1338-4325
PubMed: 28653652
WoS: 000412155000005
Scopus: 2-s2.0-85029904764
Колекције
Институција/група
VinčaTY - JOUR AU - Romić, Snježana Đ. AU - Krskova, Katarina AU - Olszanecki, Rafal AU - Balazova, Lucia AU - Lory, Viktoria AU - Korićanac, Goran AU - Slamkova, Miroslava AU - Zorad, Stefan PY - 2017 UR - https://vinar.vin.bg.ac.rs/handle/123456789/1764 AB - Fatty acid (FA) uptake and/or intramuscular triglyceride (TG) accumulation in skeletal muscle are increased in obesity, type 2 diabetes and aging. FA translocase (FAT/CD36) translocation, lipin-1 subcellular localization and nuclear factor kappa B (NF-kappa B) p65 protein content in quadriceps muscle of young and old obese Zuckerfa/fa rats and their lean controls were analyzed by immunoblot to define obesity- and aging-related alterations in FA uptake, their subsequent metabolic fate and potential to activate pro-inflammatory signaling. As expected, obesity increased FAT/CD36 content in plasma membrane in quadriceps muscle of fa/fa rats. Aging increased cytosolic lipin-1 content in both, obese rats and their lean controls. Also, old obese rats had decreased level of nuclear extract lipin-lcompared to that in old lean rats. Neither obesity nor age altered NF-kappa B p65 protein content in cytosol and nuclear extract of quadriceps muscle suggesting that obesity/aging-induced changes in FA handling are not accompanied by NF-kappa B-mediated inflammation. Increase in plasma membrane FAT/CD36 content in obese rats and failure in lipin-1 export to nucleus with progression of obesity, implying an increase in FA uptake and their different channeling into lipid intermediates synthesis pathway in old fa/fa rats versus FA usage in lean rats of the same age. T2 - General Physiology and Biophysics T1 - Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats VL - 36 IS - 4 SP - 399 EP - 406 DO - 10.4149/gpb_2017010 ER -
@article{ author = "Romić, Snježana Đ. and Krskova, Katarina and Olszanecki, Rafal and Balazova, Lucia and Lory, Viktoria and Korićanac, Goran and Slamkova, Miroslava and Zorad, Stefan", year = "2017", abstract = "Fatty acid (FA) uptake and/or intramuscular triglyceride (TG) accumulation in skeletal muscle are increased in obesity, type 2 diabetes and aging. FA translocase (FAT/CD36) translocation, lipin-1 subcellular localization and nuclear factor kappa B (NF-kappa B) p65 protein content in quadriceps muscle of young and old obese Zuckerfa/fa rats and their lean controls were analyzed by immunoblot to define obesity- and aging-related alterations in FA uptake, their subsequent metabolic fate and potential to activate pro-inflammatory signaling. As expected, obesity increased FAT/CD36 content in plasma membrane in quadriceps muscle of fa/fa rats. Aging increased cytosolic lipin-1 content in both, obese rats and their lean controls. Also, old obese rats had decreased level of nuclear extract lipin-lcompared to that in old lean rats. Neither obesity nor age altered NF-kappa B p65 protein content in cytosol and nuclear extract of quadriceps muscle suggesting that obesity/aging-induced changes in FA handling are not accompanied by NF-kappa B-mediated inflammation. Increase in plasma membrane FAT/CD36 content in obese rats and failure in lipin-1 export to nucleus with progression of obesity, implying an increase in FA uptake and their different channeling into lipid intermediates synthesis pathway in old fa/fa rats versus FA usage in lean rats of the same age.", journal = "General Physiology and Biophysics", title = "Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats", volume = "36", number = "4", pages = "399-406", doi = "10.4149/gpb_2017010" }
Romić, S. Đ., Krskova, K., Olszanecki, R., Balazova, L., Lory, V., Korićanac, G., Slamkova, M.,& Zorad, S.. (2017). Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats. in General Physiology and Biophysics, 36(4), 399-406. https://doi.org/10.4149/gpb_2017010
Romić SĐ, Krskova K, Olszanecki R, Balazova L, Lory V, Korićanac G, Slamkova M, Zorad S. Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats. in General Physiology and Biophysics. 2017;36(4):399-406. doi:10.4149/gpb_2017010 .
Romić, Snježana Đ., Krskova, Katarina, Olszanecki, Rafal, Balazova, Lucia, Lory, Viktoria, Korićanac, Goran, Slamkova, Miroslava, Zorad, Stefan, "Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats" in General Physiology and Biophysics, 36, no. 4 (2017):399-406, https://doi.org/10.4149/gpb_2017010 . .