Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions
Нема приказа
Аутори
Keta, Otilija D.Todorović, Danijela V.
Bulat, Tanja M.
Cirrone, Giuseppe Antonio Pablo
Romano, Francesco
Cuttone, Giacomo
Petrović, Ivan M.
Ristić-Fira, Aleksandra
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of this study was to investigate effects of irradiations with the therapeutic proton and carbon ion beams in two non-small cell lung cancers, CRL5876 adenocarcinoma and HTB177 large cell lung carcinoma. The DNA damage response dynamics, cell cycle regulation, and cell death pathway activation were followed. Viability of both cell lines was lower after carbon ions compared to the therapeutic proton irradiations. HTB177 cells showed higher recovery than CRL5876 cells seven days following the treatments, but the survival rates of both cell lines were lower after exposure to carbon ions with respect to therapeutic protons. When analyzing cell cycle distribution of both CRL5876 and HTB177 cells, it was noticed that therapeutic protons predominantly induced G1 arrest, while the cells after carbon ions were arrested in G2/M phase. The results illustrated that differences in the levels of phosphorylated H2AX, a double-strand break marker, exist after therapeutic proton and carbon ion i...rradiations. We also observed dose- and time-dependent increase in the p53 and p21 levels after applied irradiations. Carbon ions caused larger increase in the quantity of p53 and p21 compared to therapeutic protons. These results suggested that various repair mechanisms were induced in the treated cells. Considering the fact that we have not observed any distinct change in the Bax/Bcl-2 ratio following irradiations, it seemed that different types of cell death were involved in the response to the two types of irradiations that were applied.
Кључне речи:
Lung cancer / cell irradiation / protons / carbon ions / DNA damage / radiobiologyИзвор:
Experimental Biology and Medicine, 2017, 242, 10, 1015-1024Финансирање / пројекти:
- Радиосензитивност хуманог генома (RS-MESTD-Basic Research (BR or ON)-173046)
- Физика високих енергија са детектором CMS (RS-MESTD-Basic Research (BR or ON)-171019)
- ENSAR - European Nuclear Science and Applications Research (EU-FP7-262010)
- Istituto Nazionale di Fisica Nucleare, Laboratori Nazionali del Sud, Italy
DOI: 10.1177/1535370216669611
ISSN: 1535-3702; 1535-3699
PubMed: 27633574
WoS: 000401166100001
Scopus: 2-s2.0-85019191105
Колекције
Институција/група
VinčaTY - JOUR AU - Keta, Otilija D. AU - Todorović, Danijela V. AU - Bulat, Tanja M. AU - Cirrone, Giuseppe Antonio Pablo AU - Romano, Francesco AU - Cuttone, Giacomo AU - Petrović, Ivan M. AU - Ristić-Fira, Aleksandra PY - 2017 UR - https://vinar.vin.bg.ac.rs/handle/123456789/1573 AB - The aim of this study was to investigate effects of irradiations with the therapeutic proton and carbon ion beams in two non-small cell lung cancers, CRL5876 adenocarcinoma and HTB177 large cell lung carcinoma. The DNA damage response dynamics, cell cycle regulation, and cell death pathway activation were followed. Viability of both cell lines was lower after carbon ions compared to the therapeutic proton irradiations. HTB177 cells showed higher recovery than CRL5876 cells seven days following the treatments, but the survival rates of both cell lines were lower after exposure to carbon ions with respect to therapeutic protons. When analyzing cell cycle distribution of both CRL5876 and HTB177 cells, it was noticed that therapeutic protons predominantly induced G1 arrest, while the cells after carbon ions were arrested in G2/M phase. The results illustrated that differences in the levels of phosphorylated H2AX, a double-strand break marker, exist after therapeutic proton and carbon ion irradiations. We also observed dose- and time-dependent increase in the p53 and p21 levels after applied irradiations. Carbon ions caused larger increase in the quantity of p53 and p21 compared to therapeutic protons. These results suggested that various repair mechanisms were induced in the treated cells. Considering the fact that we have not observed any distinct change in the Bax/Bcl-2 ratio following irradiations, it seemed that different types of cell death were involved in the response to the two types of irradiations that were applied. T2 - Experimental Biology and Medicine T1 - Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions VL - 242 IS - 10 SP - 1015 EP - 1024 DO - 10.1177/1535370216669611 ER -
@article{ author = "Keta, Otilija D. and Todorović, Danijela V. and Bulat, Tanja M. and Cirrone, Giuseppe Antonio Pablo and Romano, Francesco and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra", year = "2017", abstract = "The aim of this study was to investigate effects of irradiations with the therapeutic proton and carbon ion beams in two non-small cell lung cancers, CRL5876 adenocarcinoma and HTB177 large cell lung carcinoma. The DNA damage response dynamics, cell cycle regulation, and cell death pathway activation were followed. Viability of both cell lines was lower after carbon ions compared to the therapeutic proton irradiations. HTB177 cells showed higher recovery than CRL5876 cells seven days following the treatments, but the survival rates of both cell lines were lower after exposure to carbon ions with respect to therapeutic protons. When analyzing cell cycle distribution of both CRL5876 and HTB177 cells, it was noticed that therapeutic protons predominantly induced G1 arrest, while the cells after carbon ions were arrested in G2/M phase. The results illustrated that differences in the levels of phosphorylated H2AX, a double-strand break marker, exist after therapeutic proton and carbon ion irradiations. We also observed dose- and time-dependent increase in the p53 and p21 levels after applied irradiations. Carbon ions caused larger increase in the quantity of p53 and p21 compared to therapeutic protons. These results suggested that various repair mechanisms were induced in the treated cells. Considering the fact that we have not observed any distinct change in the Bax/Bcl-2 ratio following irradiations, it seemed that different types of cell death were involved in the response to the two types of irradiations that were applied.", journal = "Experimental Biology and Medicine", title = "Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions", volume = "242", number = "10", pages = "1015-1024", doi = "10.1177/1535370216669611" }
Keta, O. D., Todorović, D. V., Bulat, T. M., Cirrone, G. A. P., Romano, F., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2017). Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions. in Experimental Biology and Medicine, 242(10), 1015-1024. https://doi.org/10.1177/1535370216669611
Keta OD, Todorović DV, Bulat TM, Cirrone GAP, Romano F, Cuttone G, Petrović IM, Ristić-Fira A. Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions. in Experimental Biology and Medicine. 2017;242(10):1015-1024. doi:10.1177/1535370216669611 .
Keta, Otilija D., Todorović, Danijela V., Bulat, Tanja M., Cirrone, Giuseppe Antonio Pablo, Romano, Francesco, Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions" in Experimental Biology and Medicine, 242, no. 10 (2017):1015-1024, https://doi.org/10.1177/1535370216669611 . .