Mechanism and Kinetics of J-Aggregation of Thiacyanine Dye in the Presence of Silver Nanoparticles
No Thumbnail
Authors
Laban, Bojana B.Vodnik, Vesna
Dramićanin, Miroslav
Novaković, Mirjana M.
Bibić, Nataša M.
Sovilj, Sofija P.
Vasić, Vesna M.
Article (Published version)
Metadata
Show full item recordAbstract
The aim of the present work was to elucidate the binding mechanism and kinetics of anionic cyanine dye 3,3-disulfopropyl-5,5-dichlorothiacyanine sodium salt (TC) J-aggregation on the surface of silver nanoparticles (AgNPs, particle size similar to 6 nm). The hybrid J-aggregateAgNPs assembly was characterized by TEM analysis, UVvis spectrophotometry, and fluorescence measurements. In the elucidation of TC binding on the surface of AgNPs, they were considered as macromolecules with several binding sites and TC dye was considered as a ligand. Scatchard and Hill analysis revealed that TC binding was a random process rather than cooperative, with similar to 200 bonded TC molecules per AgNP and a binding constant K-a = 4.8 x 10(7) M. The TC-AgNP assembly exerted concentration-dependent fluorescence quenching properties. The linearity of the SternVolmer relation, accounting for both static and dynamic quenching, indicated that only one type of quenching occurred, suggesting that AgNPs quenche...d the fluorescence of TC with an extraordinarily high SternVolmer constant (KSV) in the range of 10(8) M-1. Additionally, the kinetics of J-aggregation of TC in the presence of AgNPs was studied using a stopped flow technique. Kinetic measurements were performed as a function of the TC and AgNP concentration, yielding sigmoidal kinetic curves. The concentration dependence of the parameters of the kinetic curves indicated that J-aggregate formation on the AgNP surface occurred via a two-step process; the first was adsorption of the initial dye layer, followed by the growth of consecutive layers.
Source:
Journal of Physical Chemistry. C, 2014, 118, 40, 23393-23401Funding / projects:
- Studies of enzyme interactions with toxic and pharmacologically active molecules (RS-MESTD-Basic Research (BR or ON)-172023)
- Size-, shape- and structure- dependent properties of nanoparticles and nanocomposites (RS-MESTD-Basic Research (BR or ON)-172056)
- COST Action [MP1302]
DOI: 10.1021/jp507086g
ISSN: 1932-7447
WoS: 000343016800066
Scopus: 2-s2.0-84908134208
Collections
Institution/Community
VinčaTY - JOUR AU - Laban, Bojana B. AU - Vodnik, Vesna AU - Dramićanin, Miroslav AU - Novaković, Mirjana M. AU - Bibić, Nataša M. AU - Sovilj, Sofija P. AU - Vasić, Vesna M. PY - 2014 UR - https://vinar.vin.bg.ac.rs/handle/123456789/155 AB - The aim of the present work was to elucidate the binding mechanism and kinetics of anionic cyanine dye 3,3-disulfopropyl-5,5-dichlorothiacyanine sodium salt (TC) J-aggregation on the surface of silver nanoparticles (AgNPs, particle size similar to 6 nm). The hybrid J-aggregateAgNPs assembly was characterized by TEM analysis, UVvis spectrophotometry, and fluorescence measurements. In the elucidation of TC binding on the surface of AgNPs, they were considered as macromolecules with several binding sites and TC dye was considered as a ligand. Scatchard and Hill analysis revealed that TC binding was a random process rather than cooperative, with similar to 200 bonded TC molecules per AgNP and a binding constant K-a = 4.8 x 10(7) M. The TC-AgNP assembly exerted concentration-dependent fluorescence quenching properties. The linearity of the SternVolmer relation, accounting for both static and dynamic quenching, indicated that only one type of quenching occurred, suggesting that AgNPs quenched the fluorescence of TC with an extraordinarily high SternVolmer constant (KSV) in the range of 10(8) M-1. Additionally, the kinetics of J-aggregation of TC in the presence of AgNPs was studied using a stopped flow technique. Kinetic measurements were performed as a function of the TC and AgNP concentration, yielding sigmoidal kinetic curves. The concentration dependence of the parameters of the kinetic curves indicated that J-aggregate formation on the AgNP surface occurred via a two-step process; the first was adsorption of the initial dye layer, followed by the growth of consecutive layers. T2 - Journal of Physical Chemistry. C T1 - Mechanism and Kinetics of J-Aggregation of Thiacyanine Dye in the Presence of Silver Nanoparticles VL - 118 IS - 40 SP - 23393 EP - 23401 DO - 10.1021/jp507086g ER -
@article{ author = "Laban, Bojana B. and Vodnik, Vesna and Dramićanin, Miroslav and Novaković, Mirjana M. and Bibić, Nataša M. and Sovilj, Sofija P. and Vasić, Vesna M.", year = "2014", abstract = "The aim of the present work was to elucidate the binding mechanism and kinetics of anionic cyanine dye 3,3-disulfopropyl-5,5-dichlorothiacyanine sodium salt (TC) J-aggregation on the surface of silver nanoparticles (AgNPs, particle size similar to 6 nm). The hybrid J-aggregateAgNPs assembly was characterized by TEM analysis, UVvis spectrophotometry, and fluorescence measurements. In the elucidation of TC binding on the surface of AgNPs, they were considered as macromolecules with several binding sites and TC dye was considered as a ligand. Scatchard and Hill analysis revealed that TC binding was a random process rather than cooperative, with similar to 200 bonded TC molecules per AgNP and a binding constant K-a = 4.8 x 10(7) M. The TC-AgNP assembly exerted concentration-dependent fluorescence quenching properties. The linearity of the SternVolmer relation, accounting for both static and dynamic quenching, indicated that only one type of quenching occurred, suggesting that AgNPs quenched the fluorescence of TC with an extraordinarily high SternVolmer constant (KSV) in the range of 10(8) M-1. Additionally, the kinetics of J-aggregation of TC in the presence of AgNPs was studied using a stopped flow technique. Kinetic measurements were performed as a function of the TC and AgNP concentration, yielding sigmoidal kinetic curves. The concentration dependence of the parameters of the kinetic curves indicated that J-aggregate formation on the AgNP surface occurred via a two-step process; the first was adsorption of the initial dye layer, followed by the growth of consecutive layers.", journal = "Journal of Physical Chemistry. C", title = "Mechanism and Kinetics of J-Aggregation of Thiacyanine Dye in the Presence of Silver Nanoparticles", volume = "118", number = "40", pages = "23393-23401", doi = "10.1021/jp507086g" }
Laban, B. B., Vodnik, V., Dramićanin, M., Novaković, M. M., Bibić, N. M., Sovilj, S. P.,& Vasić, V. M.. (2014). Mechanism and Kinetics of J-Aggregation of Thiacyanine Dye in the Presence of Silver Nanoparticles. in Journal of Physical Chemistry. C, 118(40), 23393-23401. https://doi.org/10.1021/jp507086g
Laban BB, Vodnik V, Dramićanin M, Novaković MM, Bibić NM, Sovilj SP, Vasić VM. Mechanism and Kinetics of J-Aggregation of Thiacyanine Dye in the Presence of Silver Nanoparticles. in Journal of Physical Chemistry. C. 2014;118(40):23393-23401. doi:10.1021/jp507086g .
Laban, Bojana B., Vodnik, Vesna, Dramićanin, Miroslav, Novaković, Mirjana M., Bibić, Nataša M., Sovilj, Sofija P., Vasić, Vesna M., "Mechanism and Kinetics of J-Aggregation of Thiacyanine Dye in the Presence of Silver Nanoparticles" in Journal of Physical Chemistry. C, 118, no. 40 (2014):23393-23401, https://doi.org/10.1021/jp507086g . .