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dc.creatorRobajac, Dragana
dc.creatorMasnikosa, Romana
dc.creatorMikovic, Zeljko
dc.creatorNedic, Olgica
dc.date.accessioned2018-03-01T16:46:51Z
dc.date.available2018-03-01T16:46:51Z
dc.date.issued2016
dc.identifier.issn0143-4004 (print)
dc.identifier.issn1532-3102 (electronic)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/994
dc.description.abstractIntroduction: Insulin receptor (IR) and type 1 and type 2 insulin-like growth factor receptors (IGF1 R and IGF2R) play important roles in regulation of placental and foetal growth. All three receptors are abundantly glycosylated. N-glycosylation significantly affects protein conformation and may alter its function. We have recently found that the N-glycome of placental membrane proteins alters during gestation. The aim of the study presented herein was to investigate whether there were gestation-related changes in N-glycan profiles of placental IR and IGFRs. Methods: Placentas from healthy women at first (FTP) and third trimester (TTP) of pregnancy were collected, membrane proteins isolated, solubilised and used as the source of IR and IGFRs. Reactivity of glycoforms of IR and IGFRs with lectins was monitored by measuring radioactivity of I-125-ligands-receptors complexes. Results: Significant differences in the binding pattern of all three receptors to the lectins were observed between FTP and TTP, which suggest gestational changes in N-glycans bound to receptors. These changes include decrease in total fucosylated, core-fucosylated biantennary N-glycan (NA2F) and alpha 2,6-sialo-N-glycans (for IR); decrease in total fucosylated and a2,6-sialo-N-glycans and an increase in NA2F N-glycans (for IGF1R) and an increase in the total fucosylation and NA2F N-glycans (for IGF2R). Discussion: The gestational alterations in N-glycans attached to IR and IGFRs may represent a mechanism by which these receptors acquire new/additional roles as gestation progresses. (C) 2016 Elsevier Ltd. All rights reserved.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173042/RS//
dc.rightsrestrictedAccessen
dc.sourcePlacentaen
dc.subjectN-glycosylationen
dc.subjectPlacentaen
dc.subjectGestationen
dc.subjectIRen
dc.subjectIGF1Ren
dc.subjectIGF2Ren
dc.titleGestation-associated changes in the glycosylation of placental insulin and insulin-like growth factor receptorsen
dc.typearticleen
dcterms.abstractНедиц, Олгица; Робајац, Драгана; Миковиц, Зељко; Масникоса Романа;
dc.citation.volume39
dc.citation.spage70
dc.citation.epage76
dc.identifier.wos000373543800011
dc.identifier.doi10.1016/j.placenta.2016.01.005
dc.citation.rankM21
dc.identifier.pmid26992677
dc.identifier.scopus2-s2.0-84960845391


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