Antitumor activity, DNA and BSA interactions of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones
Само за регистроване кориснике
2021
Аутори
Petronijević, JelenaJoksimović, Nenad
Milović, Emilija
Đorđić Crnogorac, Marija
Petrović, Nina
Stanojković, Tatjana P.
Milivojević, Dušan
Janković, Nenad
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In order to discover new therapeutically active agents a series of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones were synthesized. All complexes were characterized by IR and EPR spectroscopic techniques and examined for their cytotoxic effect on human cancer cell lines HeLa, LS174, A549 and normal fibroblasts (MRC-5). For further examination of the cytotoxic mechanisms of novel complexes, three of them were chosen for analysing their effects on the distribution of HeLa cells in the cell cycle phases. The results of the flow cytometry analysis suggest that tested complexes lead to time-dependent accumulation of the cells in S and G2/M phases. The strongest accumulation effect showed complex 2d after 48 h of incubation. Competitive experiments with ethidium bromide (EB) indicated that tested compound 2d have affinity to displace EB from the EB-DNA complex through intercalation. Also, the binding parameters values for 2d-BSA complex showed that a reversible 2d-BSA compl...ex is formed and ligand 2d can be stored and carried by BSA.
Кључне речи:
3,4-Dihydro-2(1)-quinoxalinones / Cu(II) complexes / Cytotoxic activity / DNA and BSA interactions / Mechanism of actionИзвор:
Chemico-Biological Interactions, 2021, 348, 109647-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200122 (Универзитет у Крагујевцу, Природно-математички факултет) (RS-MESTD-inst-2020-200122)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200378 (Институт за информационе технологије, Крагујевац) (RS-MESTD-inst-2020-200378)
DOI: 10.1016/j.cbi.2021.109647
ISSN: 0009-2797
PubMed: 34520752
WoS: 000704507100006
Scopus: 2-s2.0-85114842840
Институција/група
VinčaTY - JOUR AU - Petronijević, Jelena AU - Joksimović, Nenad AU - Milović, Emilija AU - Đorđić Crnogorac, Marija AU - Petrović, Nina AU - Stanojković, Tatjana P. AU - Milivojević, Dušan AU - Janković, Nenad PY - 2021 UR - https://vinar.vin.bg.ac.rs/handle/123456789/9933 AB - In order to discover new therapeutically active agents a series of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones were synthesized. All complexes were characterized by IR and EPR spectroscopic techniques and examined for their cytotoxic effect on human cancer cell lines HeLa, LS174, A549 and normal fibroblasts (MRC-5). For further examination of the cytotoxic mechanisms of novel complexes, three of them were chosen for analysing their effects on the distribution of HeLa cells in the cell cycle phases. The results of the flow cytometry analysis suggest that tested complexes lead to time-dependent accumulation of the cells in S and G2/M phases. The strongest accumulation effect showed complex 2d after 48 h of incubation. Competitive experiments with ethidium bromide (EB) indicated that tested compound 2d have affinity to displace EB from the EB-DNA complex through intercalation. Also, the binding parameters values for 2d-BSA complex showed that a reversible 2d-BSA complex is formed and ligand 2d can be stored and carried by BSA. T2 - Chemico-Biological Interactions T1 - Antitumor activity, DNA and BSA interactions of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones VL - 348 SP - 109647 DO - 10.1016/j.cbi.2021.109647 ER -
@article{ author = "Petronijević, Jelena and Joksimović, Nenad and Milović, Emilija and Đorđić Crnogorac, Marija and Petrović, Nina and Stanojković, Tatjana P. and Milivojević, Dušan and Janković, Nenad", year = "2021", abstract = "In order to discover new therapeutically active agents a series of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones were synthesized. All complexes were characterized by IR and EPR spectroscopic techniques and examined for their cytotoxic effect on human cancer cell lines HeLa, LS174, A549 and normal fibroblasts (MRC-5). For further examination of the cytotoxic mechanisms of novel complexes, three of them were chosen for analysing their effects on the distribution of HeLa cells in the cell cycle phases. The results of the flow cytometry analysis suggest that tested complexes lead to time-dependent accumulation of the cells in S and G2/M phases. The strongest accumulation effect showed complex 2d after 48 h of incubation. Competitive experiments with ethidium bromide (EB) indicated that tested compound 2d have affinity to displace EB from the EB-DNA complex through intercalation. Also, the binding parameters values for 2d-BSA complex showed that a reversible 2d-BSA complex is formed and ligand 2d can be stored and carried by BSA.", journal = "Chemico-Biological Interactions", title = "Antitumor activity, DNA and BSA interactions of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones", volume = "348", pages = "109647", doi = "10.1016/j.cbi.2021.109647" }
Petronijević, J., Joksimović, N., Milović, E., Đorđić Crnogorac, M., Petrović, N., Stanojković, T. P., Milivojević, D.,& Janković, N.. (2021). Antitumor activity, DNA and BSA interactions of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones. in Chemico-Biological Interactions, 348, 109647. https://doi.org/10.1016/j.cbi.2021.109647
Petronijević J, Joksimović N, Milović E, Đorđić Crnogorac M, Petrović N, Stanojković TP, Milivojević D, Janković N. Antitumor activity, DNA and BSA interactions of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones. in Chemico-Biological Interactions. 2021;348:109647. doi:10.1016/j.cbi.2021.109647 .
Petronijević, Jelena, Joksimović, Nenad, Milović, Emilija, Đorđić Crnogorac, Marija, Petrović, Nina, Stanojković, Tatjana P., Milivojević, Dušan, Janković, Nenad, "Antitumor activity, DNA and BSA interactions of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones" in Chemico-Biological Interactions, 348 (2021):109647, https://doi.org/10.1016/j.cbi.2021.109647 . .