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Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles
dc.creator | Matijević, Milica | |
dc.creator | Žakula, Jelena | |
dc.creator | Korićanac, Lela | |
dc.creator | Radoičić, Marija B. | |
dc.creator | Liang, Xinyue | |
dc.creator | Mi, Lan | |
dc.creator | Filipović Tričković, Jelena G. | |
dc.creator | Valenta-Šobot, Ana | |
dc.creator | Stanković, Maja N. | |
dc.creator | Nakarada, Đura | |
dc.creator | Mojović, Miloš | |
dc.creator | Petković, Marijana | |
dc.creator | Stepić, Milutin | |
dc.creator | Nešić, Maja D. | |
dc.date.accessioned | 2021-11-12T11:54:43Z | |
dc.date.available | 2021-11-12T11:54:43Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 1474-9092 | |
dc.identifier.uri | https://vinar.vin.bg.ac.rs/handle/123456789/9908 | |
dc.description.abstract | In this study, C-doped TiO2 nanoparticles (C-TiO2) were prepared and tested as a photosensitizer for visible-light-driven photodynamic therapy against cervical cancer cells (HeLa). X-ray diffraction and Transmission Electron Microscopy confirmed the anatase form of nanoparticles, spherical shape, and size distribution from 5 to 15 nm. Ultraviolet–visible light spectroscopy showed that C doping of TiO2 enhances the optical absorption in the visible light range caused by a bandgap narrowing. The photo-cytotoxic activity of C-TiO2 was investigated in vitro against HeLa cells. The lack of dark cytotoxicity indicates good biocompatibility of C-TiO2. In contrast, a combination with blue light significantly reduced the survival of HeLa cells: illumination only decreased cell viability by 30% (15 min of illumination, 120 µW power), and 60% when HeLa cells were preincubated with C-TiO2. We have also confirmed blue light-induced C-TiO2-catalyzed generation of reactive oxygen species in vitro and intracellularly. Oxidative stress triggered by C-TiO2/blue light was the leading cause of HeLa cell death. Fluorescent labeling of treated HeLa cells showed distinct morphological changes after the C-TiO2/blue light treatment. Unlike blue light illumination, which caused the appearance of large necrotic cells with deformed nuclei, cytoplasm swelling, and membrane blebbing, a combination of C-TiO2/blue light leads to controlled cell death, thus providing a better outcome of local anticancer therapy. | en |
dc.language | en | |
dc.relation | Ministry of Education, Science and Technological Development of the Republic of Serbia | |
dc.relation | Serbian-Chinese bilateral project [451–00-478/2018–09/16, SINO-SERBIA2018002] | |
dc.rights | restrictedAccess | |
dc.source | Photochemical and Photobiological Sciences | |
dc.title | Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles | |
dc.type | article | en |
dc.rights.license | ARR | |
dc.citation.volume | 20 | |
dc.citation.issue | 8 | |
dc.citation.spage | 1087 | |
dc.citation.epage | 1098 | |
dc.identifier.wos | 000685367800001 | |
dc.identifier.doi | 10.1007/s43630-021-00082-2 | |
dc.identifier.pmid | 34398442 | |
dc.type.version | publishedVersion | |
dc.identifier.scopus | 2-s2.0-85112557363 |
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