SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives
Samo za registrovane korisnike
2020
Autori
Nešić, Maja D.Dučić, Tanja
Liang, Xinyue
Algarra, Manuel
Mi, Lan
Korićanac, Lela
Žakula, Jelena
Kop, Tatjana
Bjelaković, Mira
Mitrović, Aleksandra
Gojgić Cvijović, Gordana D.
Stepić, Milutin
Petković, Marijana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Objects of the present study are improved fullerene C60 drug carrier properties trough encapsulation by microbial polysaccharides, levan (LEV), pullulan (PUL), and their hydrophobized cholesterol-derivatives (CHL and CHP), that show better interaction with cancer cells. The zeta potential, polydispersity index, and the diameter of particles were determined, and their cytotoxicity against three cancer cell lines were tested. Biochemical changes in HeLa cells are analyzed by synchrotron radiation (SR) FTIR spectro-microscopy combined with the principal component analysis (PCA). The most significant changes occur in HeLa cells treated with LEV-C60 and correspond to the changes in the protein region, i.e. Amide I band, and the changes in the structure of lipid bodies and membrane fluidity are evident. The highest cytotoxicity was also induced by LEV-C60. In HeLa cells, cytotoxicity could not be strictly associated with biochemical changes in lipids, proteins and nucleic acids, but these fi...ndings are significant contribution to the study of the mechanism of interaction of C60-based nanoparticles with cellular biomolecules. In conclusion, LEV, PUL, CHL, and CHP enhanced fullerene C60 potential to be used as target drug delivery system with the ability to induce specific intracellular changes in HeLa cancer cells.
Ključne reči:
Levan-C / Pullulan-C / Synchrotron radiation FTIR spectro-microscopyIzvor:
International Journal of Biological Macromolecules, 2020, 165, 2541-2549Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200017 (Univerzitet u Beogradu, Institut za nuklearne nauke Vinča, Beograd-Vinča) (RS-MESTD-inst-2020-200017)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200026 (Univerzitet u Beogradu, Institut za hemiju, tehnologiju i metalurgiju - IHTM) (RS-MESTD-inst-2020-200026)
- Serbian-Chinese bilateral project [451-00-478/2018-09/16]
- Serbian-Chinese bilateral project [SINO-SER-BIA2018002]
- Portuguese Foundation for Science and Technology [PEstOE/QUI/UI0674/2019, UID/MAT/00006/2019, INNOINDIGO/0001/2015, RAM-M1420-01-0145-FEDER-000008]
- ARDITI [M1420-01-0145-FEDER-000005]
- Ministerio de Ciencia, Innovacion y Universidades [RTI2018-099668-BC22]
- Junta de Andalucia [UMA18-FEDERJA-126]
- European Commission [2019093770]
DOI: 10.1016/j.ijbiomac.2020.10.141
ISSN: 0141-8130
PubMed: 33736274
WoS: 000600773500088
Scopus: 2-s2.0-85094207129
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Nešić, Maja D. AU - Dučić, Tanja AU - Liang, Xinyue AU - Algarra, Manuel AU - Mi, Lan AU - Korićanac, Lela AU - Žakula, Jelena AU - Kop, Tatjana AU - Bjelaković, Mira AU - Mitrović, Aleksandra AU - Gojgić Cvijović, Gordana D. AU - Stepić, Milutin AU - Petković, Marijana PY - 2020 UR - https://vinar.vin.bg.ac.rs/handle/123456789/9712 AB - Objects of the present study are improved fullerene C60 drug carrier properties trough encapsulation by microbial polysaccharides, levan (LEV), pullulan (PUL), and their hydrophobized cholesterol-derivatives (CHL and CHP), that show better interaction with cancer cells. The zeta potential, polydispersity index, and the diameter of particles were determined, and their cytotoxicity against three cancer cell lines were tested. Biochemical changes in HeLa cells are analyzed by synchrotron radiation (SR) FTIR spectro-microscopy combined with the principal component analysis (PCA). The most significant changes occur in HeLa cells treated with LEV-C60 and correspond to the changes in the protein region, i.e. Amide I band, and the changes in the structure of lipid bodies and membrane fluidity are evident. The highest cytotoxicity was also induced by LEV-C60. In HeLa cells, cytotoxicity could not be strictly associated with biochemical changes in lipids, proteins and nucleic acids, but these findings are significant contribution to the study of the mechanism of interaction of C60-based nanoparticles with cellular biomolecules. In conclusion, LEV, PUL, CHL, and CHP enhanced fullerene C60 potential to be used as target drug delivery system with the ability to induce specific intracellular changes in HeLa cancer cells. T2 - International Journal of Biological Macromolecules T1 - SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives VL - 165 SP - 2541 EP - 2549 DO - 10.1016/j.ijbiomac.2020.10.141 ER -
@article{ author = "Nešić, Maja D. and Dučić, Tanja and Liang, Xinyue and Algarra, Manuel and Mi, Lan and Korićanac, Lela and Žakula, Jelena and Kop, Tatjana and Bjelaković, Mira and Mitrović, Aleksandra and Gojgić Cvijović, Gordana D. and Stepić, Milutin and Petković, Marijana", year = "2020", abstract = "Objects of the present study are improved fullerene C60 drug carrier properties trough encapsulation by microbial polysaccharides, levan (LEV), pullulan (PUL), and their hydrophobized cholesterol-derivatives (CHL and CHP), that show better interaction with cancer cells. The zeta potential, polydispersity index, and the diameter of particles were determined, and their cytotoxicity against three cancer cell lines were tested. Biochemical changes in HeLa cells are analyzed by synchrotron radiation (SR) FTIR spectro-microscopy combined with the principal component analysis (PCA). The most significant changes occur in HeLa cells treated with LEV-C60 and correspond to the changes in the protein region, i.e. Amide I band, and the changes in the structure of lipid bodies and membrane fluidity are evident. The highest cytotoxicity was also induced by LEV-C60. In HeLa cells, cytotoxicity could not be strictly associated with biochemical changes in lipids, proteins and nucleic acids, but these findings are significant contribution to the study of the mechanism of interaction of C60-based nanoparticles with cellular biomolecules. In conclusion, LEV, PUL, CHL, and CHP enhanced fullerene C60 potential to be used as target drug delivery system with the ability to induce specific intracellular changes in HeLa cancer cells.", journal = "International Journal of Biological Macromolecules", title = "SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives", volume = "165", pages = "2541-2549", doi = "10.1016/j.ijbiomac.2020.10.141" }
Nešić, M. D., Dučić, T., Liang, X., Algarra, M., Mi, L., Korićanac, L., Žakula, J., Kop, T., Bjelaković, M., Mitrović, A., Gojgić Cvijović, G. D., Stepić, M.,& Petković, M.. (2020). SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives. in International Journal of Biological Macromolecules, 165, 2541-2549. https://doi.org/10.1016/j.ijbiomac.2020.10.141
Nešić MD, Dučić T, Liang X, Algarra M, Mi L, Korićanac L, Žakula J, Kop T, Bjelaković M, Mitrović A, Gojgić Cvijović GD, Stepić M, Petković M. SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives. in International Journal of Biological Macromolecules. 2020;165:2541-2549. doi:10.1016/j.ijbiomac.2020.10.141 .
Nešić, Maja D., Dučić, Tanja, Liang, Xinyue, Algarra, Manuel, Mi, Lan, Korićanac, Lela, Žakula, Jelena, Kop, Tatjana, Bjelaković, Mira, Mitrović, Aleksandra, Gojgić Cvijović, Gordana D., Stepić, Milutin, Petković, Marijana, "SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives" in International Journal of Biological Macromolecules, 165 (2020):2541-2549, https://doi.org/10.1016/j.ijbiomac.2020.10.141 . .