Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry
Аутори
Houdová, DominikaSoto, Juan
Castro, Rita
Rodrigues, João
Soledad Pino-González, Ma
Petković, Marijana
Bandosz, Teresa J
Algarra, Manuel
Чланак у часопису (Рецензирана верзија)
Метаподаци
Приказ свих података о документуАпстракт
A binary system composed of carbon dots (CDs) and N-doped CDs (N-CDs) embedded in an organic matrix was used for the analysis of cholesterol by MALDI (matrix-assisted laser desorption and ionization time-of-flight) mass spectrometry, as a model for detection of small, biologically relevant molecules. The results showed that both CDs are sensitive to the cholesterol and can be used either alone or in a binary system with 2,5-dihydroxybenzoic acid (DHB) to enhance the detection process. It was found that both COOH and NH2 groups on CDs surface contributed to the enhancement in the cholesterol detection by MALDI mass spectrometry in the presence of inorganic cations. Nevertheless, in the presence of NaCl, N-CDs led to a better reproducibility of results. It was due to the coexistence of positive and negative charge on N-CDs surface that led to a homogeneous analyte/substrate distribution, which is an important detection parameter. The enhancing effect of carbon dots was linked to a negati...ve Gibbs energy of the complex formation between CDs, Na+, cholesterol and DHB, and it was supported by theoretical calculations. Moreover, upon the addition of CDs/N-CDs, such features as a low ionization potential, vertical excitation, dipole moment and oscillator strength positively affected the cholesterol detection by MALDI in the presence of Na+. © 2021 Elsevier Inc.
Кључне речи:
Cholesterol / MALDI TOF / Carbon dots / N-doped Carbon dots / DFT calculationsИзвор:
Journal of Colloid and Interface Science, 2021, 591, 373-383Финансирање / пројекти:
- Spanish Ministerio de Economia, Industria y Competividad [RTI2018-099668-BC22]
- Portuguese Foundation for Science and Technology European Commission [UIDB/00674/2020]
- ARDITI [M1420-010145-FEDER-000005 -CQM+]
- ARDITI [M142009-5369-FSE-000002]
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200017 (Универзитет у Београду, Институт за нуклеарне науке Винча, Београд-Винча) (RS-200017)
- Programa de Cooperacion Territorial INTERREG V-A MAC 2014-2020 through the Project Inv2Mac [MAC2/4.6d/229]
Напомена:
- This is accepted peer-reviewed version of the article: Houdová, D., Soto, J., Castro, R., Rodrigues, J., Pino-González, M. S., Petković, M., ... & Algarra, M. (2021). Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry. Journal of Colloid and Interface Science, 591, 373-383. DOI:http://dx.doi.org/10.1016/j.jcis.2021.02.004
Повезане информације:
- Друга верзија
https://vinar.vin.bg.ac.rs/handle/123456789/9127
DOI: 10.1016/j.jcis.2021.02.004
ISSN: 1095-7103
PubMed: 33631525
WoS: 000632774800008
Scopus: 2-s2.0-85101097876
Институција/група
VinčaTY - JOUR AU - Houdová, Dominika AU - Soto, Juan AU - Castro, Rita AU - Rodrigues, João AU - Soledad Pino-González, Ma AU - Petković, Marijana AU - Bandosz, Teresa J AU - Algarra, Manuel PY - 2021 UR - https://vinar.vin.bg.ac.rs/handle/123456789/9145 AB - A binary system composed of carbon dots (CDs) and N-doped CDs (N-CDs) embedded in an organic matrix was used for the analysis of cholesterol by MALDI (matrix-assisted laser desorption and ionization time-of-flight) mass spectrometry, as a model for detection of small, biologically relevant molecules. The results showed that both CDs are sensitive to the cholesterol and can be used either alone or in a binary system with 2,5-dihydroxybenzoic acid (DHB) to enhance the detection process. It was found that both COOH and NH2 groups on CDs surface contributed to the enhancement in the cholesterol detection by MALDI mass spectrometry in the presence of inorganic cations. Nevertheless, in the presence of NaCl, N-CDs led to a better reproducibility of results. It was due to the coexistence of positive and negative charge on N-CDs surface that led to a homogeneous analyte/substrate distribution, which is an important detection parameter. The enhancing effect of carbon dots was linked to a negative Gibbs energy of the complex formation between CDs, Na+, cholesterol and DHB, and it was supported by theoretical calculations. Moreover, upon the addition of CDs/N-CDs, such features as a low ionization potential, vertical excitation, dipole moment and oscillator strength positively affected the cholesterol detection by MALDI in the presence of Na+. © 2021 Elsevier Inc. T2 - Journal of Colloid and Interface Science T1 - Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry VL - 591 SP - 373 EP - 383 DO - 10.1016/j.jcis.2021.02.004 ER -
@article{ author = "Houdová, Dominika and Soto, Juan and Castro, Rita and Rodrigues, João and Soledad Pino-González, Ma and Petković, Marijana and Bandosz, Teresa J and Algarra, Manuel", year = "2021", abstract = "A binary system composed of carbon dots (CDs) and N-doped CDs (N-CDs) embedded in an organic matrix was used for the analysis of cholesterol by MALDI (matrix-assisted laser desorption and ionization time-of-flight) mass spectrometry, as a model for detection of small, biologically relevant molecules. The results showed that both CDs are sensitive to the cholesterol and can be used either alone or in a binary system with 2,5-dihydroxybenzoic acid (DHB) to enhance the detection process. It was found that both COOH and NH2 groups on CDs surface contributed to the enhancement in the cholesterol detection by MALDI mass spectrometry in the presence of inorganic cations. Nevertheless, in the presence of NaCl, N-CDs led to a better reproducibility of results. It was due to the coexistence of positive and negative charge on N-CDs surface that led to a homogeneous analyte/substrate distribution, which is an important detection parameter. The enhancing effect of carbon dots was linked to a negative Gibbs energy of the complex formation between CDs, Na+, cholesterol and DHB, and it was supported by theoretical calculations. Moreover, upon the addition of CDs/N-CDs, such features as a low ionization potential, vertical excitation, dipole moment and oscillator strength positively affected the cholesterol detection by MALDI in the presence of Na+. © 2021 Elsevier Inc.", journal = "Journal of Colloid and Interface Science", title = "Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry", volume = "591", pages = "373-383", doi = "10.1016/j.jcis.2021.02.004" }
Houdová, D., Soto, J., Castro, R., Rodrigues, J., Soledad Pino-González, M., Petković, M., Bandosz, T. J.,& Algarra, M.. (2021). Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry. in Journal of Colloid and Interface Science, 591, 373-383. https://doi.org/10.1016/j.jcis.2021.02.004
Houdová D, Soto J, Castro R, Rodrigues J, Soledad Pino-González M, Petković M, Bandosz TJ, Algarra M. Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry. in Journal of Colloid and Interface Science. 2021;591:373-383. doi:10.1016/j.jcis.2021.02.004 .
Houdová, Dominika, Soto, Juan, Castro, Rita, Rodrigues, João, Soledad Pino-González, Ma, Petković, Marijana, Bandosz, Teresa J, Algarra, Manuel, "Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry" in Journal of Colloid and Interface Science, 591 (2021):373-383, https://doi.org/10.1016/j.jcis.2021.02.004 . .