Cell wall response to UV radiation in needles of Picea omorika
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2021
Authors
Mitrović, Aleksandra Lj.Simonović-Radosavljević, Jasna
Prokopijević, Miloš
Spasojević, Dragica
Kovačević, Jovana
Prodanović, Olivera
Todorović, Bratislav
Matović, Branko

Stanković, Mira
Maksimović, Vuk

Mutavdžić, Dragosav
Skočić, Miloš
Pešić, Mirjana
Prokić, Ljiljana
Radotić, Ksenija

Article (Published version)

© 2021 Elsevier
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The UV-B represents the minor fraction of the solar spectrum, while UV-C is not contained in natural solar radiation, but both radiation types can cause damaging effects in plants. Cell walls (CWs) are one of the targets for external stressors. Juvenile P. omorika trees were treated either with 21 day-high doses UV-B or with 7 day- UV-C in open-top chambers. Using spectroscopic and biochemical techniques, it was shown that the response to UV radiation includes numerous modifications in needle CW structure: relative content of xylan, xyloglucan, lignin and cellulose decreased; cellulose crystallinity changed; yield of lignin monomers with stronger connection of C[dbnd]C in side chain with the ring increased; re-distribution of inter- and intra-polymer H-bonds occurred. The recovery was mediated by an increase in the activities and changes in isoform profiles of CW bound covalent peroxidases (POD) and polyphenol oxidases (PO) (UV–B), and ionic POD and covalent PO (UV–C). A connection bet...ween activities of specific POD/PO isoforms and phenolic species (m- and p-coumaric acid, pinoresinol and cinnamic acid derivatives) was demonstrated, and supported by changes in the sRNA profile. In vivo fluorometry showed phenolics accumulation in needle epidermal CWs. These results imply transversal connections between polymers and changed mechanical properties of needle CW as a response to UV. The CW alterations enabled maintenance of physiological functions, as indicated by the preserved chlorophyll content and/or organization. The current study provides evidence that in conifers, needle CW response to both UV-B and UV-C includes biochemical modifications and structural remodeling. © 2021 Elsevier Masson SAS
Keywords:
Cell wall polymers / Peroxidases / Phenolics / Picea omorika / Polyphenol oxidases / UV radiationSource:
Plant Physiology and Biochemistry, 2021, 161, 176-190Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200053 (University of Belgrade, Institute for Multidisciplinary Research) (RS-200053)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200116 (University of Belgrade, Faculty of Agriculture) (RS-200116)
DOI: 10.1016/j.plaphy.2021.02.007
ISSN: 0981-9428
PubMed: 33618201
WoS: 000637955100018
Scopus: 2-s2.0-85101082745
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VinčaTY - JOUR AU - Mitrović, Aleksandra Lj. AU - Simonović-Radosavljević, Jasna AU - Prokopijević, Miloš AU - Spasojević, Dragica AU - Kovačević, Jovana AU - Prodanović, Olivera AU - Todorović, Bratislav AU - Matović, Branko AU - Stanković, Mira AU - Maksimović, Vuk AU - Mutavdžić, Dragosav AU - Skočić, Miloš AU - Pešić, Mirjana AU - Prokić, Ljiljana AU - Radotić, Ksenija PY - 2021 UR - https://vinar.vin.bg.ac.rs/handle/123456789/9129 AB - The UV-B represents the minor fraction of the solar spectrum, while UV-C is not contained in natural solar radiation, but both radiation types can cause damaging effects in plants. Cell walls (CWs) are one of the targets for external stressors. Juvenile P. omorika trees were treated either with 21 day-high doses UV-B or with 7 day- UV-C in open-top chambers. Using spectroscopic and biochemical techniques, it was shown that the response to UV radiation includes numerous modifications in needle CW structure: relative content of xylan, xyloglucan, lignin and cellulose decreased; cellulose crystallinity changed; yield of lignin monomers with stronger connection of C[dbnd]C in side chain with the ring increased; re-distribution of inter- and intra-polymer H-bonds occurred. The recovery was mediated by an increase in the activities and changes in isoform profiles of CW bound covalent peroxidases (POD) and polyphenol oxidases (PO) (UV–B), and ionic POD and covalent PO (UV–C). A connection between activities of specific POD/PO isoforms and phenolic species (m- and p-coumaric acid, pinoresinol and cinnamic acid derivatives) was demonstrated, and supported by changes in the sRNA profile. In vivo fluorometry showed phenolics accumulation in needle epidermal CWs. These results imply transversal connections between polymers and changed mechanical properties of needle CW as a response to UV. The CW alterations enabled maintenance of physiological functions, as indicated by the preserved chlorophyll content and/or organization. The current study provides evidence that in conifers, needle CW response to both UV-B and UV-C includes biochemical modifications and structural remodeling. © 2021 Elsevier Masson SAS T2 - Plant Physiology and Biochemistry T1 - Cell wall response to UV radiation in needles of Picea omorika VL - 161 SP - 176 EP - 190 DO - 10.1016/j.plaphy.2021.02.007 ER -
@article{ author = "Mitrović, Aleksandra Lj. and Simonović-Radosavljević, Jasna and Prokopijević, Miloš and Spasojević, Dragica and Kovačević, Jovana and Prodanović, Olivera and Todorović, Bratislav and Matović, Branko and Stanković, Mira and Maksimović, Vuk and Mutavdžić, Dragosav and Skočić, Miloš and Pešić, Mirjana and Prokić, Ljiljana and Radotić, Ksenija", year = "2021", abstract = "The UV-B represents the minor fraction of the solar spectrum, while UV-C is not contained in natural solar radiation, but both radiation types can cause damaging effects in plants. Cell walls (CWs) are one of the targets for external stressors. Juvenile P. omorika trees were treated either with 21 day-high doses UV-B or with 7 day- UV-C in open-top chambers. Using spectroscopic and biochemical techniques, it was shown that the response to UV radiation includes numerous modifications in needle CW structure: relative content of xylan, xyloglucan, lignin and cellulose decreased; cellulose crystallinity changed; yield of lignin monomers with stronger connection of C[dbnd]C in side chain with the ring increased; re-distribution of inter- and intra-polymer H-bonds occurred. The recovery was mediated by an increase in the activities and changes in isoform profiles of CW bound covalent peroxidases (POD) and polyphenol oxidases (PO) (UV–B), and ionic POD and covalent PO (UV–C). A connection between activities of specific POD/PO isoforms and phenolic species (m- and p-coumaric acid, pinoresinol and cinnamic acid derivatives) was demonstrated, and supported by changes in the sRNA profile. In vivo fluorometry showed phenolics accumulation in needle epidermal CWs. These results imply transversal connections between polymers and changed mechanical properties of needle CW as a response to UV. The CW alterations enabled maintenance of physiological functions, as indicated by the preserved chlorophyll content and/or organization. The current study provides evidence that in conifers, needle CW response to both UV-B and UV-C includes biochemical modifications and structural remodeling. © 2021 Elsevier Masson SAS", journal = "Plant Physiology and Biochemistry", title = "Cell wall response to UV radiation in needles of Picea omorika", volume = "161", pages = "176-190", doi = "10.1016/j.plaphy.2021.02.007" }
Mitrović, A. Lj., Simonović-Radosavljević, J., Prokopijević, M., Spasojević, D., Kovačević, J., Prodanović, O., Todorović, B., Matović, B., Stanković, M., Maksimović, V., Mutavdžić, D., Skočić, M., Pešić, M., Prokić, L.,& Radotić, K.. (2021). Cell wall response to UV radiation in needles of Picea omorika. in Plant Physiology and Biochemistry, 161, 176-190. https://doi.org/10.1016/j.plaphy.2021.02.007
Mitrović AL, Simonović-Radosavljević J, Prokopijević M, Spasojević D, Kovačević J, Prodanović O, Todorović B, Matović B, Stanković M, Maksimović V, Mutavdžić D, Skočić M, Pešić M, Prokić L, Radotić K. Cell wall response to UV radiation in needles of Picea omorika. in Plant Physiology and Biochemistry. 2021;161:176-190. doi:10.1016/j.plaphy.2021.02.007 .
Mitrović, Aleksandra Lj., Simonović-Radosavljević, Jasna, Prokopijević, Miloš, Spasojević, Dragica, Kovačević, Jovana, Prodanović, Olivera, Todorović, Bratislav, Matović, Branko, Stanković, Mira, Maksimović, Vuk, Mutavdžić, Dragosav, Skočić, Miloš, Pešić, Mirjana, Prokić, Ljiljana, Radotić, Ksenija, "Cell wall response to UV radiation in needles of Picea omorika" in Plant Physiology and Biochemistry, 161 (2021):176-190, https://doi.org/10.1016/j.plaphy.2021.02.007 . .