Cloning and expression profiling of muscle regulator ANKRD2 in domestic chicken Gallus gallus
Само за регистроване кориснике
2020
Аутори
Stamenković, NemanjaJasnić, Jovana
Novković, Mirjana
Milošević, Emilija
Bošković, Srđan
Kojić, Ana
Popić, Kristina
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Stanković, Marija
Wang, Yajun
Milenković, Sanja
Radojković, Dragica
Ma, Guoda
Kojić, Snežana
Чланак у часопису (Објављена верзија)
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© 2020, Springer-Verlag GmbH Germany, part of Springer Nature
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Приказ свих података о документуАпстракт
Striated muscle signaling protein and transcriptional regulator ANKRD2 participates in myogenesis, myogenic differentiation, muscle adaptation and stress response. It is preferentially expressed in slow, oxidative fibers of mammalian skeletal muscle. In this study, we report on characterization of chicken ANKRD2. The chicken ANKRD2 coding region contains 1002 bp and encodes a 334-amino acid protein which shares approximately 58% identity with human and mouse orthologs, mostly in the conserved region of ankyrin repeats. Comprehensive analysis of the ANKRD2 gene and protein expression in adult chicken demonstrated its predominant expression in red muscles of thigh and drumstick, compared to white muscle. It was not detected in heart and white pectoral muscle. Uneven expression of ANKRD2 in chicken skeletal muscles, observed by immunohistochemistry, was attributed to its selective expression in slow, oxidative, type I and fast, oxidative–glycolytic, type IIA myofibers. Association of chic...ken ANKRD2 with phenotypic differences between red and white muscles points to its potential role in the process of myofiber-type specification. In addition to expression in slow oxidative myofibers, as demonstrated for mammalian protein, chicken ANKRD2 was also detected in fast fibers with mixed oxidative and glycolytic metabolism. This finding suggests that ANKRD2 is responsive to metabolic differences between types of avian myofibers and orientates future studies towards investigation of its role in molecular mechanisms of myofiber-type-specific gene expression.
Кључне речи:
ANKRD2 / Chicken / Slow muscle fiber / Oxidative metabolism / Skeletal muscleИзвор:
Histochemistry and Cell Biology, 2020, 154, 4, 383-396Финансирање / пројекти:
- Комплексне болести као модел систем за проучавање модулације фенотипа-структурна и функционална анализа молекуларних биомаркера (RS-MESTD-Basic Research (BR or ON)-173008)
- Ministry of Education, Science and Technological Development of the Republic of Serbia [337-00-125/2015-09/13]
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
- Ministry of Science and Technology, China [3-13]
- National Natural Science Foundation of China (NSFC) [81670252]
- National Natural Science Foundation of China (NSFC)[81770034]
- Guangdong Basic and Applied Basic Foundation [2019A1515011306]
DOI: 10.1007/s00418-020-01899-1
ISSN: 0948-6143
PubMed: 32653935
WoS: 000547349500002
Scopus: 2-s2.0-85087666926
Институција/група
VinčaTY - JOUR AU - Stamenković, Nemanja AU - Jasnić, Jovana AU - Novković, Mirjana AU - Milošević, Emilija AU - Bošković, Srđan AU - Kojić, Ana AU - Popić, Kristina AU - Stanković, Marija AU - Wang, Yajun AU - Milenković, Sanja AU - Radojković, Dragica AU - Ma, Guoda AU - Kojić, Snežana PY - 2020 UR - https://vinar.vin.bg.ac.rs/handle/123456789/9077 AB - Striated muscle signaling protein and transcriptional regulator ANKRD2 participates in myogenesis, myogenic differentiation, muscle adaptation and stress response. It is preferentially expressed in slow, oxidative fibers of mammalian skeletal muscle. In this study, we report on characterization of chicken ANKRD2. The chicken ANKRD2 coding region contains 1002 bp and encodes a 334-amino acid protein which shares approximately 58% identity with human and mouse orthologs, mostly in the conserved region of ankyrin repeats. Comprehensive analysis of the ANKRD2 gene and protein expression in adult chicken demonstrated its predominant expression in red muscles of thigh and drumstick, compared to white muscle. It was not detected in heart and white pectoral muscle. Uneven expression of ANKRD2 in chicken skeletal muscles, observed by immunohistochemistry, was attributed to its selective expression in slow, oxidative, type I and fast, oxidative–glycolytic, type IIA myofibers. Association of chicken ANKRD2 with phenotypic differences between red and white muscles points to its potential role in the process of myofiber-type specification. In addition to expression in slow oxidative myofibers, as demonstrated for mammalian protein, chicken ANKRD2 was also detected in fast fibers with mixed oxidative and glycolytic metabolism. This finding suggests that ANKRD2 is responsive to metabolic differences between types of avian myofibers and orientates future studies towards investigation of its role in molecular mechanisms of myofiber-type-specific gene expression. T2 - Histochemistry and Cell Biology T1 - Cloning and expression profiling of muscle regulator ANKRD2 in domestic chicken Gallus gallus VL - 154 IS - 4 SP - 383 EP - 396 DO - 10.1007/s00418-020-01899-1 ER -
@article{ author = "Stamenković, Nemanja and Jasnić, Jovana and Novković, Mirjana and Milošević, Emilija and Bošković, Srđan and Kojić, Ana and Popić, Kristina and Stanković, Marija and Wang, Yajun and Milenković, Sanja and Radojković, Dragica and Ma, Guoda and Kojić, Snežana", year = "2020", abstract = "Striated muscle signaling protein and transcriptional regulator ANKRD2 participates in myogenesis, myogenic differentiation, muscle adaptation and stress response. It is preferentially expressed in slow, oxidative fibers of mammalian skeletal muscle. In this study, we report on characterization of chicken ANKRD2. The chicken ANKRD2 coding region contains 1002 bp and encodes a 334-amino acid protein which shares approximately 58% identity with human and mouse orthologs, mostly in the conserved region of ankyrin repeats. Comprehensive analysis of the ANKRD2 gene and protein expression in adult chicken demonstrated its predominant expression in red muscles of thigh and drumstick, compared to white muscle. It was not detected in heart and white pectoral muscle. Uneven expression of ANKRD2 in chicken skeletal muscles, observed by immunohistochemistry, was attributed to its selective expression in slow, oxidative, type I and fast, oxidative–glycolytic, type IIA myofibers. Association of chicken ANKRD2 with phenotypic differences between red and white muscles points to its potential role in the process of myofiber-type specification. In addition to expression in slow oxidative myofibers, as demonstrated for mammalian protein, chicken ANKRD2 was also detected in fast fibers with mixed oxidative and glycolytic metabolism. This finding suggests that ANKRD2 is responsive to metabolic differences between types of avian myofibers and orientates future studies towards investigation of its role in molecular mechanisms of myofiber-type-specific gene expression.", journal = "Histochemistry and Cell Biology", title = "Cloning and expression profiling of muscle regulator ANKRD2 in domestic chicken Gallus gallus", volume = "154", number = "4", pages = "383-396", doi = "10.1007/s00418-020-01899-1" }
Stamenković, N., Jasnić, J., Novković, M., Milošević, E., Bošković, S., Kojić, A., Popić, K., Stanković, M., Wang, Y., Milenković, S., Radojković, D., Ma, G.,& Kojić, S.. (2020). Cloning and expression profiling of muscle regulator ANKRD2 in domestic chicken Gallus gallus. in Histochemistry and Cell Biology, 154(4), 383-396. https://doi.org/10.1007/s00418-020-01899-1
Stamenković N, Jasnić J, Novković M, Milošević E, Bošković S, Kojić A, Popić K, Stanković M, Wang Y, Milenković S, Radojković D, Ma G, Kojić S. Cloning and expression profiling of muscle regulator ANKRD2 in domestic chicken Gallus gallus. in Histochemistry and Cell Biology. 2020;154(4):383-396. doi:10.1007/s00418-020-01899-1 .
Stamenković, Nemanja, Jasnić, Jovana, Novković, Mirjana, Milošević, Emilija, Bošković, Srđan, Kojić, Ana, Popić, Kristina, Stanković, Marija, Wang, Yajun, Milenković, Sanja, Radojković, Dragica, Ma, Guoda, Kojić, Snežana, "Cloning and expression profiling of muscle regulator ANKRD2 in domestic chicken Gallus gallus" in Histochemistry and Cell Biology, 154, no. 4 (2020):383-396, https://doi.org/10.1007/s00418-020-01899-1 . .