Analytical techniques for multiplex analysis of protein biomarkers
Authors
Van Gool, AlainCorrales, Fernado
Čolović, Mirjana B.

Krstić, Danijela Z.

Oliver-Martos, Begona
Martínez-Cáceres, Eva
Jakasa, Ivone
Gajski, Goran
Brun, Virginie
Kyriacou, Kyriacos
Burzynska-Pedziwiatr, Izabela
Wozniak, Lucyna Alicja
Nierkens, Stephan
Pascual García, César
Katrlik, Jaroslav
Bojić-Trbojević, Žanka
Vacek, Jan
Llorente, Alicia
Antohe, Felicia
Suica, Viorel
Suarez, Guillaume
T’Kindt, Ruben
Martin, Petra
Penque, Deborah
Martins, Ines Lanca
Bodoki, Ede
Iacob, Bogdan-Cezar
Aydindogan, Eda
Timur, Suna
Allinson, John
Sutton, Christopher
Luider, Theo
Wittfooth, Saara
Sammar, Marei
Review (Published version)
Metadata
Show full item recordAbstract
Introduction: The importance of biomarkers for pharmaceutical drug development and clinical diagnostics is more significant than ever in the current shift toward personalized medicine. Biomarkers have taken a central position either as companion markers to support drug development and patient selection, or as indicators aiming to detect the earliest perturbations indicative of disease, minimizing therapeutic intervention or even enabling disease reversal. Protein biomarkers are of particular interest given their central role in biochemical pathways. Hence, capabilities to analyze multiple protein biomarkers in one assay are highly interesting for biomedical research. Areas covered: We here review multiple methods that are suitable for robust, high throughput, standardized, and affordable analysis of protein biomarkers in a multiplex format. We describe innovative developments in immunoassays, the vanguard of methods in clinical laboratories, and mass spectrometry, increasingly implemen...ted for protein biomarker analysis. Moreover, emerging techniques are discussed with potentially improved protein capture, separation, and detection that will further boost multiplex analyses. Expert commentary: The development of clinically applied multiplex protein biomarker assays is essential as multi-protein signatures provide more comprehensive information about biological systems than single biomarkers, leading to improved insights in mechanisms of disease, diagnostics, and the effect of personalized medicine.
Keywords:
multiplexing / mass spectrometry / validationSource:
Expert Review of Proteomics, 2020, 17, 4, 257-273Funding / projects:
- COST Action [CA16113]
DOI: 10.1080/14789450.2020.1763174
ISSN: 1478-9450
PubMed: 32427033
WoS: 000536384300001
Scopus: 2-s2.0-85085325196
Institution/Community
VinčaTY - JOUR AU - Van Gool, Alain AU - Corrales, Fernado AU - Čolović, Mirjana B. AU - Krstić, Danijela Z. AU - Oliver-Martos, Begona AU - Martínez-Cáceres, Eva AU - Jakasa, Ivone AU - Gajski, Goran AU - Brun, Virginie AU - Kyriacou, Kyriacos AU - Burzynska-Pedziwiatr, Izabela AU - Wozniak, Lucyna Alicja AU - Nierkens, Stephan AU - Pascual García, César AU - Katrlik, Jaroslav AU - Bojić-Trbojević, Žanka AU - Vacek, Jan AU - Llorente, Alicia AU - Antohe, Felicia AU - Suica, Viorel AU - Suarez, Guillaume AU - T’Kindt, Ruben AU - Martin, Petra AU - Penque, Deborah AU - Martins, Ines Lanca AU - Bodoki, Ede AU - Iacob, Bogdan-Cezar AU - Aydindogan, Eda AU - Timur, Suna AU - Allinson, John AU - Sutton, Christopher AU - Luider, Theo AU - Wittfooth, Saara AU - Sammar, Marei PY - 2020 UR - https://vinar.vin.bg.ac.rs/handle/123456789/9018 AB - Introduction: The importance of biomarkers for pharmaceutical drug development and clinical diagnostics is more significant than ever in the current shift toward personalized medicine. Biomarkers have taken a central position either as companion markers to support drug development and patient selection, or as indicators aiming to detect the earliest perturbations indicative of disease, minimizing therapeutic intervention or even enabling disease reversal. Protein biomarkers are of particular interest given their central role in biochemical pathways. Hence, capabilities to analyze multiple protein biomarkers in one assay are highly interesting for biomedical research. Areas covered: We here review multiple methods that are suitable for robust, high throughput, standardized, and affordable analysis of protein biomarkers in a multiplex format. We describe innovative developments in immunoassays, the vanguard of methods in clinical laboratories, and mass spectrometry, increasingly implemented for protein biomarker analysis. Moreover, emerging techniques are discussed with potentially improved protein capture, separation, and detection that will further boost multiplex analyses. Expert commentary: The development of clinically applied multiplex protein biomarker assays is essential as multi-protein signatures provide more comprehensive information about biological systems than single biomarkers, leading to improved insights in mechanisms of disease, diagnostics, and the effect of personalized medicine. T2 - Expert Review of Proteomics T1 - Analytical techniques for multiplex analysis of protein biomarkers VL - 17 IS - 4 SP - 257 EP - 273 DO - 10.1080/14789450.2020.1763174 ER -
@article{ author = "Van Gool, Alain and Corrales, Fernado and Čolović, Mirjana B. and Krstić, Danijela Z. and Oliver-Martos, Begona and Martínez-Cáceres, Eva and Jakasa, Ivone and Gajski, Goran and Brun, Virginie and Kyriacou, Kyriacos and Burzynska-Pedziwiatr, Izabela and Wozniak, Lucyna Alicja and Nierkens, Stephan and Pascual García, César and Katrlik, Jaroslav and Bojić-Trbojević, Žanka and Vacek, Jan and Llorente, Alicia and Antohe, Felicia and Suica, Viorel and Suarez, Guillaume and T’Kindt, Ruben and Martin, Petra and Penque, Deborah and Martins, Ines Lanca and Bodoki, Ede and Iacob, Bogdan-Cezar and Aydindogan, Eda and Timur, Suna and Allinson, John and Sutton, Christopher and Luider, Theo and Wittfooth, Saara and Sammar, Marei", year = "2020", abstract = "Introduction: The importance of biomarkers for pharmaceutical drug development and clinical diagnostics is more significant than ever in the current shift toward personalized medicine. Biomarkers have taken a central position either as companion markers to support drug development and patient selection, or as indicators aiming to detect the earliest perturbations indicative of disease, minimizing therapeutic intervention or even enabling disease reversal. Protein biomarkers are of particular interest given their central role in biochemical pathways. Hence, capabilities to analyze multiple protein biomarkers in one assay are highly interesting for biomedical research. Areas covered: We here review multiple methods that are suitable for robust, high throughput, standardized, and affordable analysis of protein biomarkers in a multiplex format. We describe innovative developments in immunoassays, the vanguard of methods in clinical laboratories, and mass spectrometry, increasingly implemented for protein biomarker analysis. Moreover, emerging techniques are discussed with potentially improved protein capture, separation, and detection that will further boost multiplex analyses. Expert commentary: The development of clinically applied multiplex protein biomarker assays is essential as multi-protein signatures provide more comprehensive information about biological systems than single biomarkers, leading to improved insights in mechanisms of disease, diagnostics, and the effect of personalized medicine.", journal = "Expert Review of Proteomics", title = "Analytical techniques for multiplex analysis of protein biomarkers", volume = "17", number = "4", pages = "257-273", doi = "10.1080/14789450.2020.1763174" }
Van Gool, A., Corrales, F., Čolović, M. B., Krstić, D. Z., Oliver-Martos, B., Martínez-Cáceres, E., Jakasa, I., Gajski, G., Brun, V., Kyriacou, K., Burzynska-Pedziwiatr, I., Wozniak, L. A., Nierkens, S., Pascual García, C., Katrlik, J., Bojić-Trbojević, Ž., Vacek, J., Llorente, A., Antohe, F., Suica, V., Suarez, G., T’Kindt, R., Martin, P., Penque, D., Martins, I. L., Bodoki, E., Iacob, B., Aydindogan, E., Timur, S., Allinson, J., Sutton, C., Luider, T., Wittfooth, S.,& Sammar, M.. (2020). Analytical techniques for multiplex analysis of protein biomarkers. in Expert Review of Proteomics, 17(4), 257-273. https://doi.org/10.1080/14789450.2020.1763174
Van Gool A, Corrales F, Čolović MB, Krstić DZ, Oliver-Martos B, Martínez-Cáceres E, Jakasa I, Gajski G, Brun V, Kyriacou K, Burzynska-Pedziwiatr I, Wozniak LA, Nierkens S, Pascual García C, Katrlik J, Bojić-Trbojević Ž, Vacek J, Llorente A, Antohe F, Suica V, Suarez G, T’Kindt R, Martin P, Penque D, Martins IL, Bodoki E, Iacob B, Aydindogan E, Timur S, Allinson J, Sutton C, Luider T, Wittfooth S, Sammar M. Analytical techniques for multiplex analysis of protein biomarkers. in Expert Review of Proteomics. 2020;17(4):257-273. doi:10.1080/14789450.2020.1763174 .
Van Gool, Alain, Corrales, Fernado, Čolović, Mirjana B., Krstić, Danijela Z., Oliver-Martos, Begona, Martínez-Cáceres, Eva, Jakasa, Ivone, Gajski, Goran, Brun, Virginie, Kyriacou, Kyriacos, Burzynska-Pedziwiatr, Izabela, Wozniak, Lucyna Alicja, Nierkens, Stephan, Pascual García, César, Katrlik, Jaroslav, Bojić-Trbojević, Žanka, Vacek, Jan, Llorente, Alicia, Antohe, Felicia, Suica, Viorel, Suarez, Guillaume, T’Kindt, Ruben, Martin, Petra, Penque, Deborah, Martins, Ines Lanca, Bodoki, Ede, Iacob, Bogdan-Cezar, Aydindogan, Eda, Timur, Suna, Allinson, John, Sutton, Christopher, Luider, Theo, Wittfooth, Saara, Sammar, Marei, "Analytical techniques for multiplex analysis of protein biomarkers" in Expert Review of Proteomics, 17, no. 4 (2020):257-273, https://doi.org/10.1080/14789450.2020.1763174 . .