Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines
Authors
Keta, Otilija D.
Deljanin, Milena
Petković, Vladana

Zdunić, Gordana
Janković, Teodora

Živković, Jelena
Ristić-Fira, Aleksandra

Petrović, Ivan M.

Šavikin, Katarina
Article (Published version)

© 2020 ACG Publications
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The aim of the present study was to investigate effects of pomegranate peel (PP) extract on different human cancer cell lines. MTT was performed to estimate cytotoxic effects of PP extract against HTB140, HTB177, MCF7, HCT116 human cancer cell lines and MRC-5 normal fibroblasts. Clonogenic assay was used to reveal cell survival after the treatment with PP extract. Cell cycle analysis was done using flow cytometry. Wound healing assay was applied to estimate inhibitory effects of PP extract on migration of cancer cells. The results showed that PP extract expressed selective cytotoxicity for cancer cells compared to normal cell line. Analyzed cancer cell lines displayed individual variations in sensitivity to PP extract reflected through changes in clonogenic survival, cell cycle distribution and migration, which may be due to the specific nature of each tested cell line. In conclusion, PP extract exhibits good inhibitory effects on tested cancer cell lines.
Keywords:
Punica granatum L. / peel extract / phenolic compounds / cytotoxicity / cancer cellsSource:
Records of Natural Products, 2020, 14, 5, 326-339Funding / projects:
- Traditional and new products of cultivated and wild growing fruits and grape vines, and by-products durring processing, with special emphasis on indigenous varieties: chemical characterization and biological profile (RS-46013)
- Radiosensitivity of human genome (RS-173046)
- High Energy Physics with the CMS Detector (RS-171019)
DOI: 10.25135/rnp.170.19.11.1477
ISSN: 1307-6167
WoS: 000531162200003
Scopus: 2-s2.0-85084426886
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VinčaTY - JOUR AU - Keta, Otilija D. AU - Deljanin, Milena AU - Petković, Vladana AU - Zdunić, Gordana AU - Janković, Teodora AU - Živković, Jelena AU - Ristić-Fira, Aleksandra AU - Petrović, Ivan M. AU - Šavikin, Katarina PY - 2020 UR - https://vinar.vin.bg.ac.rs/handle/123456789/8996 AB - The aim of the present study was to investigate effects of pomegranate peel (PP) extract on different human cancer cell lines. MTT was performed to estimate cytotoxic effects of PP extract against HTB140, HTB177, MCF7, HCT116 human cancer cell lines and MRC-5 normal fibroblasts. Clonogenic assay was used to reveal cell survival after the treatment with PP extract. Cell cycle analysis was done using flow cytometry. Wound healing assay was applied to estimate inhibitory effects of PP extract on migration of cancer cells. The results showed that PP extract expressed selective cytotoxicity for cancer cells compared to normal cell line. Analyzed cancer cell lines displayed individual variations in sensitivity to PP extract reflected through changes in clonogenic survival, cell cycle distribution and migration, which may be due to the specific nature of each tested cell line. In conclusion, PP extract exhibits good inhibitory effects on tested cancer cell lines. T2 - Records of Natural Products T1 - Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines VL - 14 IS - 5 SP - 326 EP - 339 DO - 10.25135/rnp.170.19.11.1477 ER -
@article{ author = "Keta, Otilija D. and Deljanin, Milena and Petković, Vladana and Zdunić, Gordana and Janković, Teodora and Živković, Jelena and Ristić-Fira, Aleksandra and Petrović, Ivan M. and Šavikin, Katarina", year = "2020", abstract = "The aim of the present study was to investigate effects of pomegranate peel (PP) extract on different human cancer cell lines. MTT was performed to estimate cytotoxic effects of PP extract against HTB140, HTB177, MCF7, HCT116 human cancer cell lines and MRC-5 normal fibroblasts. Clonogenic assay was used to reveal cell survival after the treatment with PP extract. Cell cycle analysis was done using flow cytometry. Wound healing assay was applied to estimate inhibitory effects of PP extract on migration of cancer cells. The results showed that PP extract expressed selective cytotoxicity for cancer cells compared to normal cell line. Analyzed cancer cell lines displayed individual variations in sensitivity to PP extract reflected through changes in clonogenic survival, cell cycle distribution and migration, which may be due to the specific nature of each tested cell line. In conclusion, PP extract exhibits good inhibitory effects on tested cancer cell lines.", journal = "Records of Natural Products", title = "Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines", volume = "14", number = "5", pages = "326-339", doi = "10.25135/rnp.170.19.11.1477" }
Keta, O. D., Deljanin, M., Petković, V., Zdunić, G., Janković, T., Živković, J., Ristić-Fira, A., Petrović, I. M.,& Šavikin, K.. (2020). Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines. in Records of Natural Products, 14(5), 326-339. https://doi.org/10.25135/rnp.170.19.11.1477
Keta OD, Deljanin M, Petković V, Zdunić G, Janković T, Živković J, Ristić-Fira A, Petrović IM, Šavikin K. Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines. in Records of Natural Products. 2020;14(5):326-339. doi:10.25135/rnp.170.19.11.1477 .
Keta, Otilija D., Deljanin, Milena, Petković, Vladana, Zdunić, Gordana, Janković, Teodora, Živković, Jelena, Ristić-Fira, Aleksandra, Petrović, Ivan M., Šavikin, Katarina, "Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines" in Records of Natural Products, 14, no. 5 (2020):326-339, https://doi.org/10.25135/rnp.170.19.11.1477 . .