Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction
Само за регистроване кориснике
2020
Аутори
Micić, MilutinAntonijević, Đorđe
Milutinović-Smiljanić, Sanja
Trišić, Dijana
Čolović, Božana M.
Kosanović, Dejana
Prokić, Bogomir Bolka
Vasić, Jugoslav
Živković, Slavoljub
Milašin, Jelena
Danilović, Vesna
Đurić, Marija P.
Jokanović, Vukoman R.
Чланак у часопису (Објављена верзија)
,
© 2020 Walter de Gruyter GmbH, Berlin/Boston
Метаподаци
Приказ свих података о документуАпстракт
The aim of this study was to develop novel hydroxyapatite (HAP)-based bioactive bone replacement materials for segmental osteotomy reconstruction. Customized three-dimensional (3D) bone construct was manufactured from nanohydroxyapatite (nHAP) with poly(lactide-co-glycolide) (PLGA) coating using 3D models derived from the computed tomography (CT) scanning of the rabbit's ulna and gradient 3D printing of the bone substitute mimicking the anatomical shape of the natural bone defect. Engineered construct revealed adequate micro-architectural design for successful bone regeneration having a total porosity of 64% and an average pore size of 256 μm. Radiography and micro-CT analysis depicted new bone apposition through the whole length of the reconstructed ulna with a small area of non-resorbed construct in the central area of defect. Histological analysis revealed new bone formation with both endochondral and endesmal type of ossification. Immunohistochemistry analysis depicted the presence... of bone formation indicators-bone morphogenetic protein (BMP), osteocalcin (OCN) and osteopontin (OPN) within newly formed bone. Manufactured personalized construct acts as a "smart" responsive biomaterial capable of modulating the functionality and potential for the personalized bone reconstruction on a clinically relevant length scale.
Кључне речи:
3D printing / bone reconstruction / personalized medicine / tissue engineeringИзвор:
Biomedizinische Technik, 2020, 65, 4, 491-505Финансирање / пројекти:
- Хемијско и структурно дизајнирање наноматеријала за примену у медицини и инжењерству ткива (RS-MESTD-Basic Research (BR or ON)-172026)
- Функционални, функционализовани и усавршени нано материјали (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45005)
Напомена:
- Link to erratum: https://vinar.vin.bg.ac.rs/handle/123456789/10363
Повезане информације:
- Повезани садржај
https://vinar.vin.bg.ac.rs/handle/123456789/10363
DOI: 10.1515/bmt-2019-0218
ISSN: 0013-5585
PubMed: 32335536
WoS: 000560600600012
Scopus: 2-s2.0-85084702256
Институција/група
VinčaTY - JOUR AU - Micić, Milutin AU - Antonijević, Đorđe AU - Milutinović-Smiljanić, Sanja AU - Trišić, Dijana AU - Čolović, Božana M. AU - Kosanović, Dejana AU - Prokić, Bogomir Bolka AU - Vasić, Jugoslav AU - Živković, Slavoljub AU - Milašin, Jelena AU - Danilović, Vesna AU - Đurić, Marija P. AU - Jokanović, Vukoman R. PY - 2020 UR - https://vinar.vin.bg.ac.rs/handle/123456789/8993 AB - The aim of this study was to develop novel hydroxyapatite (HAP)-based bioactive bone replacement materials for segmental osteotomy reconstruction. Customized three-dimensional (3D) bone construct was manufactured from nanohydroxyapatite (nHAP) with poly(lactide-co-glycolide) (PLGA) coating using 3D models derived from the computed tomography (CT) scanning of the rabbit's ulna and gradient 3D printing of the bone substitute mimicking the anatomical shape of the natural bone defect. Engineered construct revealed adequate micro-architectural design for successful bone regeneration having a total porosity of 64% and an average pore size of 256 μm. Radiography and micro-CT analysis depicted new bone apposition through the whole length of the reconstructed ulna with a small area of non-resorbed construct in the central area of defect. Histological analysis revealed new bone formation with both endochondral and endesmal type of ossification. Immunohistochemistry analysis depicted the presence of bone formation indicators-bone morphogenetic protein (BMP), osteocalcin (OCN) and osteopontin (OPN) within newly formed bone. Manufactured personalized construct acts as a "smart" responsive biomaterial capable of modulating the functionality and potential for the personalized bone reconstruction on a clinically relevant length scale. T2 - Biomedizinische Technik T1 - Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction VL - 65 IS - 4 SP - 491 EP - 505 DO - 10.1515/bmt-2019-0218 ER -
@article{ author = "Micić, Milutin and Antonijević, Đorđe and Milutinović-Smiljanić, Sanja and Trišić, Dijana and Čolović, Božana M. and Kosanović, Dejana and Prokić, Bogomir Bolka and Vasić, Jugoslav and Živković, Slavoljub and Milašin, Jelena and Danilović, Vesna and Đurić, Marija P. and Jokanović, Vukoman R.", year = "2020", abstract = "The aim of this study was to develop novel hydroxyapatite (HAP)-based bioactive bone replacement materials for segmental osteotomy reconstruction. Customized three-dimensional (3D) bone construct was manufactured from nanohydroxyapatite (nHAP) with poly(lactide-co-glycolide) (PLGA) coating using 3D models derived from the computed tomography (CT) scanning of the rabbit's ulna and gradient 3D printing of the bone substitute mimicking the anatomical shape of the natural bone defect. Engineered construct revealed adequate micro-architectural design for successful bone regeneration having a total porosity of 64% and an average pore size of 256 μm. Radiography and micro-CT analysis depicted new bone apposition through the whole length of the reconstructed ulna with a small area of non-resorbed construct in the central area of defect. Histological analysis revealed new bone formation with both endochondral and endesmal type of ossification. Immunohistochemistry analysis depicted the presence of bone formation indicators-bone morphogenetic protein (BMP), osteocalcin (OCN) and osteopontin (OPN) within newly formed bone. Manufactured personalized construct acts as a "smart" responsive biomaterial capable of modulating the functionality and potential for the personalized bone reconstruction on a clinically relevant length scale.", journal = "Biomedizinische Technik", title = "Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction", volume = "65", number = "4", pages = "491-505", doi = "10.1515/bmt-2019-0218" }
Micić, M., Antonijević, Đ., Milutinović-Smiljanić, S., Trišić, D., Čolović, B. M., Kosanović, D., Prokić, B. B., Vasić, J., Živković, S., Milašin, J., Danilović, V., Đurić, M. P.,& Jokanović, V. R.. (2020). Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction. in Biomedizinische Technik, 65(4), 491-505. https://doi.org/10.1515/bmt-2019-0218
Micić M, Antonijević Đ, Milutinović-Smiljanić S, Trišić D, Čolović BM, Kosanović D, Prokić BB, Vasić J, Živković S, Milašin J, Danilović V, Đurić MP, Jokanović VR. Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction. in Biomedizinische Technik. 2020;65(4):491-505. doi:10.1515/bmt-2019-0218 .
Micić, Milutin, Antonijević, Đorđe, Milutinović-Smiljanić, Sanja, Trišić, Dijana, Čolović, Božana M., Kosanović, Dejana, Prokić, Bogomir Bolka, Vasić, Jugoslav, Živković, Slavoljub, Milašin, Jelena, Danilović, Vesna, Đurić, Marija P., Jokanović, Vukoman R., "Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction" in Biomedizinische Technik, 65, no. 4 (2020):491-505, https://doi.org/10.1515/bmt-2019-0218 . .