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dc.creatorZec, Manja
dc.creatorStojković, Ljiljana S.
dc.creatorZeković, Milica
dc.creatorPokimica, Biljana
dc.creatorŽivković, Maja
dc.creatorStanković, Aleksandra
dc.creatorGlibetić, Marija
dc.date.accessioned2020-12-24T09:02:20Z
dc.date.available2020-12-24T09:02:20Z
dc.date.issued2020
dc.identifier.issn0271-5317
dc.identifier.urihttps://vinar.vin.bg.ac.rs/handle/123456789/8883
dc.description.abstractPolymorphisms in FADS genes are associated with plasma long-chain polyunsaturated fatty acids (LC-PUFA) and modulate omega-6/omega-3 balance. We hypothesized that the FADS2 gene variants will be associated with lower product-to-precursor ratio in the fatty acid metabolic pathways. Thus, we explored FADS2 rs174593, rs174616, and rs174576 effects on plasma phospholipid fatty acid profile, markers of desaturase activities, and risk factors in a sample of apparently healthy Serbian adults. Food and nutrient intake data were compiled through 24 h recalls. Plasma phospholipid fatty acid content was assessed by gas-chromatography. Estimated desaturase activities were calculated as conversion rates towards LC-PUFA in omega-6 pathway. During the selection of FADS2 polymorphisms, we accounted for their positional and functional aspect. Genotyping was performed by Real-Time PCR. Multivariable-adjusted general linear and hierarchical regression models were applied. Study subjects (mean age = 40 ± 7 years, 70% who were overweight) had a median dietary omega-6/omega-3 ratio of 16.29. Alternative allele frequencies were 33%, 36%, and 51% for rs174593, rs174576, and rs174616, respectively. Addition of FADS2 alternative alleles was associated with lower plasma arachidonic acid (AA, C20:4 n-6, P < .001) and estimated desaturase-5 activity (P < .001), irrespective of gender, age, daily polyunsaturated/saturated fatty acid intake, and obesity. The rs174576 association with AA withstood multiple testing and additional adjustments for other variants (multivariable-adjusted β = −1.14 [95% CI: −2.25, −0.43]). None of the variants was associated with dietary intake, serum lipids, or obesity. We observed inverse associations between FADS2 variants and plasma AA but not omega-3 fatty acids in Serbian subjects, with rs174576 exhibiting the strongest relation.en
dc.language.isoen
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/312090/EU//
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200015/RS//
dc.rightsrestrictedAccess
dc.sourceNutrition Research
dc.subjectArachidonicaciden
dc.subjectDesaturase-5en
dc.subjectFatty aciddesaturasegenesen
dc.subjectOmega-6/omega-3intakeen
dc.subjectPrecision nutritionen
dc.subjectPolyunsaturatedfattyacidsen
dc.titleFADS2 polymorphisms are associated with plasma arachidonic acid and estimated desaturase-5 activity in a cross-sectional studyen
dc.typearticleen
dc.rights.licenseARR
dcterms.abstractПокимица, Биљана; Стојковић, Љиљана С.; Зец, Мања; Живковић, Маја; Станковић, Aлександра; Зековић, Милица; Глибетић, Марија;
dc.rights.holder© 2020 Elsevier Inc.
dc.citation.volume83
dc.citation.spage49
dc.citation.epage62
dc.identifier.wos000591510000004
dc.identifier.doi10.1016/j.nutres.2020.08.010
dc.identifier.pmid33011673
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-85092004550


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