Ruthenium(II) bipyridine complexes: from synthesis and crystal structures to electrochemical and cytotoxicity investigation
AuthorsBaroud, Afya A.
Mihajlović-Lalić, Ljiljana E.
Gligorijević, Nevenka N.
Stanković, Dalibor M.
Radulović, Siniša S.
Van Hecke, Kristof
Article (Accepted Version)
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Complexes 1-4, [Ru(L)(bpy)(2)]PF6, where bpy=2,2-bipyridine; HL=3-methylpyridine-2-carboxylic acid (HL1), 6-methylpyridine-2-carboxylic acid (HL2), 5-bromopyridine-2-carboxylic acid (HL3) and 6-bromopyridine-2-carboxylic acid (HL4), were synthesized and characterized. The electrochemical character of the complexes was investigated by cyclic voltammetry revealing two reversible reduction waves in the negative range of potentials, most likely due to a reduction of the bipyridine moiety. Cytotoxicity studies by MTT assay for 72h of drug action revealed that 2-4 exhibited moderate activity in cervical human tumor cells (HeLa). Complex 2 exhibited low activity in colon cancer LS-174 cells (180 +/- 10), while all complexes were devoid of activity in lung cancer A549 and non-tumor MRC-5 cells, up to 200M. Combinational studies of the most active complex 2, with pharmacological modulators of cell redox status, L-buthionine-sulfoximine (L-BSO) or N-acetyl-L-cysteine (NAC), showed that when L-BS...O potentiated, 2 induced a sub-G1 peak of the cell cycle in the HeLa cell line. UV-vis and cyclic voltammetry were performed in order to investigate the binding mode of 2 to DNA and suggested intercalation for the complex-DNA interaction. [GRAPHICS]
Keywords:Ruthenium(II) bipyridine complexes / crystal structure / redox properties / cytotoxicity / DNA intercalation
Source:Journal of Coordination Chemistry, 2017, 70, 5, 831-847
- Reinforcement of the Faculty of Chemistry, University of Belgrade, towards becoming a Center of Excellence in the region of WB for Molecular Biotechnology and Food research (EU-256716)
- Rational design and synthesis of biologically active and coordination compounds and functional materials, relevant for (bio)nanotechnology (RS-172035)
- Pharmacodynamic and pharmacogenomic research of new drugs in the treatment of solid tumors (RS-41026)
- Hercules Foundation [AUGE/11/029]
- Research Foundation - Flanders (FWO) [18.104.22.168N]
- Strengthening of the MagBioVin Research and Innovation Team for Development of Novel Approaches for Tumour Therapy based on Nanostructured Materials (EU-621375)
- This is accepted peer-reviewed version of the article: Baroud, A. A., Mihajlović-Lalić, L. E., Gligorijević, N., Aranđelović, S., Stanković, D., Radulović, S., ... & Grgurić-Šipka, S. (2017). Ruthenium (II) bipyridine complexes: from synthesis and crystal structures to electrochemical and cytotoxicity investigation. Journal of Coordination Chemistry, 70(5), 831-847. https://dx.doi.org/10.1080/00958972.2017.1282611
- Published version: https://vinar.vin.bg.ac.rs/handle/123456789/1438