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dc.creatorRomić, Snježana Đ.
dc.creatorĐorđević, Ana
dc.creatorTepavčević, Snežana
dc.creatorĆulafić, Tijana
dc.creatorStojiljković, Mojca
dc.creatorBursać, Biljana
dc.creatorStanišić, Jelena
dc.creatorKostić, Milan
dc.creatorGligorovska, Ljupka
dc.creatorKorićanac, Goran
dc.date.accessioned2020-11-03T10:17:28Z
dc.date.available2020-11-03T10:17:28Z
dc.date.issued2020
dc.identifier.issn2042-6496
dc.identifier.urihttps://vinar.vin.bg.ac.rs/handle/123456789/8849
dc.description.abstractBoth a diet rich in fructose and chronic stress exposure induce metabolic and cardiovascular disturbances. The aim of this study was to examine the effects of the fructose-rich diet and chronic stress, separately and in combination, on insulin signaling and molecules regulating glycogen synthesis and ion transport in the heart, and to reveal whether these effects coincide with changes in glucocorticoid receptor (GR) activation. Male Wistar rats were subjected to 10% fructose in drinking water and/or to chronic unpredictable stress for 9 weeks. Protein expression and/or phosphorylation of the insulin receptor (IR), protein tyrosine phosphatase 1B, insulin receptor substrate 1 (IRS1), protein kinase B (Akt), extracellular signal-regulated kinase 1/2 (ERK1/2), glycogen synthase kinase-3β (GSK-3β) and Na+/K+-ATPase α-subunits in cardiac tissue were analyzed by western blot. GR distribution between cytosolic and nuclear fractions was also analyzed. The fructose-rich diet decreased the level of pERK1/2 (Thr202/Tyr204) and pGSK-3β (Ser9) independently of stress, while chronic stress increased the IRS1 content and prevented the fructose diet-induced decrease of the pAkt (Ser473) level. The fructose-rich diet in combination with chronic stress reduced the protein content of cardiac IR and attenuated IRS1 upregulation. Separate treatments increased the protein content of Na+/K+-ATPase α1- and α2-subunits, while after combined treatment the α2 content was at the control level and the α1 content was lower than the control level. The effect of combined treatment on cardiac IR and α2-subunit expression could be mediated by increased GR nuclear accumulation. Our study provides new insights into the effects of chronic stress and a combination of the fructose diet and chronic stress on the studied molecules in the heart.en
dc.language.isoen
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41009/RS//
dc.rightsrestrictedAccess
dc.sourceFood & Function
dc.titleEffects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male ratsen
dc.typearticleen
dc.rights.licenseARR
dcterms.abstractЋулафић, Тијана; Стојиљковић, Мојца; Бурсаћ, Биљана; Глигоровска, Љупка; Костић, Милан; Станишић, Јелена; Тепавчевић, Снежана; Ђорђевић, Aна; Ромић, Сњежана; Корићанац, Горан;
dc.rights.holder© The Royal Society of Chemistry 2020
dc.citation.volume11
dc.citation.issue2
dc.citation.spage1455
dc.citation.epage1466
dc.identifier.wos000518566700021
dc.identifier.doi10.1039/C9FO02306B
dc.citation.rankM21~
dc.identifier.pmid31974538
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-85080932543


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