Anti-human albumin monoclonal antibody immobilized on EDC-NHS functionalized carboxylic graphene/AuNPs composite as promising electrochemical HSA immunosensor
Samo za registrovane korisnike
2020
Autori
Stanković, VesnaĐurđić, Slađana
Ognjanović, Miloš
Antić, Bratislav
Kalcher, Kurt
Mutić, Jelena J.
Stanković, Dalibor M.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Diagnosis and treatment of some important diseased and metabolic disorders is based on successful detection of albumin. In this work, we aim to develop a simple immunosensor for the detection of human serum albumin in biological fluids. Anti-human albumin antibody was covalently attached to the activated surface of screen-printed carbon electrodes enriched with carboxyl graphene/gold nanoparticles composite. Microstructure (TEM, FE-SEM, XRD) and electrochemical (CV, EIS) characterization methods were used to investigate composite properties and to confirm the successful modification of the electrodes. Under the optimal conditions, linear working range and limit of detection were 2.5–500 μg/mL and 1.55 μg/mL, respectively. Additionally, the effect of some possibly interfering compounds was investigated and the immunosensor was used for real sample analysis. The results showed that the sensor exhibited accurate, precise and sensitive characteristics and can be promising replacement to th...e convention methods for albumin detection in clinical practice.
Ključne reči:
Electrochemical biosensor / Human serum albumin / Immunosensor / Screen-printed carbon electrodeIzvor:
Journal of Electroanalytical Chemistry, 2020, 860, 113928-Finansiranje / projekti:
- Primena unapređenih oksidacionih procesa i nanostrukturisanih oksidnih materijala za uklanjanje zagađivača iz životne sredine, razvoj i optimizacija instrumentalnih tehnika za praćenje efikasnosti (RS-MESTD-Basic Research (BR or ON)-172030)
- CEEPUS networkCIII-CZ-0212-12-1819-M-120151 (Education of Modern Analytical and Bioanalytical Methods).
- EUREKA [E! 13303 MED-BIO-TEST]
Napomena:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3892
- Peer-reviewed manuscript: http://cherry.chem.bg.ac.rs/handle/123456789/3891
DOI: 10.1016/j.jelechem.2020.113928
ISSN: 1572-6657
WoS: 000519336100030
Scopus: 2-s2.0-85079132277
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Stanković, Vesna AU - Đurđić, Slađana AU - Ognjanović, Miloš AU - Antić, Bratislav AU - Kalcher, Kurt AU - Mutić, Jelena J. AU - Stanković, Dalibor M. PY - 2020 UR - https://vinar.vin.bg.ac.rs/handle/123456789/8819 AB - Diagnosis and treatment of some important diseased and metabolic disorders is based on successful detection of albumin. In this work, we aim to develop a simple immunosensor for the detection of human serum albumin in biological fluids. Anti-human albumin antibody was covalently attached to the activated surface of screen-printed carbon electrodes enriched with carboxyl graphene/gold nanoparticles composite. Microstructure (TEM, FE-SEM, XRD) and electrochemical (CV, EIS) characterization methods were used to investigate composite properties and to confirm the successful modification of the electrodes. Under the optimal conditions, linear working range and limit of detection were 2.5–500 μg/mL and 1.55 μg/mL, respectively. Additionally, the effect of some possibly interfering compounds was investigated and the immunosensor was used for real sample analysis. The results showed that the sensor exhibited accurate, precise and sensitive characteristics and can be promising replacement to the convention methods for albumin detection in clinical practice. T2 - Journal of Electroanalytical Chemistry T1 - Anti-human albumin monoclonal antibody immobilized on EDC-NHS functionalized carboxylic graphene/AuNPs composite as promising electrochemical HSA immunosensor VL - 860 SP - 113928 DO - 10.1016/j.jelechem.2020.113928 ER -
@article{ author = "Stanković, Vesna and Đurđić, Slađana and Ognjanović, Miloš and Antić, Bratislav and Kalcher, Kurt and Mutić, Jelena J. and Stanković, Dalibor M.", year = "2020", abstract = "Diagnosis and treatment of some important diseased and metabolic disorders is based on successful detection of albumin. In this work, we aim to develop a simple immunosensor for the detection of human serum albumin in biological fluids. Anti-human albumin antibody was covalently attached to the activated surface of screen-printed carbon electrodes enriched with carboxyl graphene/gold nanoparticles composite. Microstructure (TEM, FE-SEM, XRD) and electrochemical (CV, EIS) characterization methods were used to investigate composite properties and to confirm the successful modification of the electrodes. Under the optimal conditions, linear working range and limit of detection were 2.5–500 μg/mL and 1.55 μg/mL, respectively. Additionally, the effect of some possibly interfering compounds was investigated and the immunosensor was used for real sample analysis. The results showed that the sensor exhibited accurate, precise and sensitive characteristics and can be promising replacement to the convention methods for albumin detection in clinical practice.", journal = "Journal of Electroanalytical Chemistry", title = "Anti-human albumin monoclonal antibody immobilized on EDC-NHS functionalized carboxylic graphene/AuNPs composite as promising electrochemical HSA immunosensor", volume = "860", pages = "113928", doi = "10.1016/j.jelechem.2020.113928" }
Stanković, V., Đurđić, S., Ognjanović, M., Antić, B., Kalcher, K., Mutić, J. J.,& Stanković, D. M.. (2020). Anti-human albumin monoclonal antibody immobilized on EDC-NHS functionalized carboxylic graphene/AuNPs composite as promising electrochemical HSA immunosensor. in Journal of Electroanalytical Chemistry, 860, 113928. https://doi.org/10.1016/j.jelechem.2020.113928
Stanković V, Đurđić S, Ognjanović M, Antić B, Kalcher K, Mutić JJ, Stanković DM. Anti-human albumin monoclonal antibody immobilized on EDC-NHS functionalized carboxylic graphene/AuNPs composite as promising electrochemical HSA immunosensor. in Journal of Electroanalytical Chemistry. 2020;860:113928. doi:10.1016/j.jelechem.2020.113928 .
Stanković, Vesna, Đurđić, Slađana, Ognjanović, Miloš, Antić, Bratislav, Kalcher, Kurt, Mutić, Jelena J., Stanković, Dalibor M., "Anti-human albumin monoclonal antibody immobilized on EDC-NHS functionalized carboxylic graphene/AuNPs composite as promising electrochemical HSA immunosensor" in Journal of Electroanalytical Chemistry, 860 (2020):113928, https://doi.org/10.1016/j.jelechem.2020.113928 . .