Biocompatible fluorescent nanostructures were prepared by a functionalization of gold nanoparticles with the amino acid tryptophan. The gold-tryptophan bioconjugates were investigated by TEM and HRTEM and various spectroscopy methods (XPS, FTIR, UV-vis and photoluminescence). It was found that the gold nanoparticles, initially 8 nm in diameter, aggregate in the presence of the amino acid. From the XPS and FTIR spectroscopy results, it was concluded that the tryptophan gold interactions mainly take place via indole and carboxyl groups. Although the indole group is involved in the interaction with the gold surfaces, the tryptophan-gold hybrids showed strong fluorescence due to the presence of multilayers of tryptophan. Deep ultra violet (DUV) imaging performed at the SOLEIL synchrotron showed that It is possible to detect these hybrid nanostructures within Escherichia coli cells. (c) 2015 Elsevier B.V. All rights reserved.
TY - JOUR
AU - Pajović, Jelena D.
AU - Dojčilović, Radovan
AU - Božanić, Dušan K.
AU - Kascakova, Slavka
AU - Refregiers, Matthieu
AU - Dimitrijević-Branković, Suzana I.
AU - Vodnik, Vesna
AU - Milosavljević, Aleksandar R.
AU - Piscopiello, Emanuela
AU - Luyt, Adriaan S.
AU - Đoković, Vladimir
PY - 2015
UR - http://vinar.vin.bg.ac.rs/handle/123456789/875
AB - Biocompatible fluorescent nanostructures were prepared by a functionalization of gold nanoparticles with the amino acid tryptophan. The gold-tryptophan bioconjugates were investigated by TEM and HRTEM and various spectroscopy methods (XPS, FTIR, UV-vis and photoluminescence). It was found that the gold nanoparticles, initially 8 nm in diameter, aggregate in the presence of the amino acid. From the XPS and FTIR spectroscopy results, it was concluded that the tryptophan gold interactions mainly take place via indole and carboxyl groups. Although the indole group is involved in the interaction with the gold surfaces, the tryptophan-gold hybrids showed strong fluorescence due to the presence of multilayers of tryptophan. Deep ultra violet (DUV) imaging performed at the SOLEIL synchrotron showed that It is possible to detect these hybrid nanostructures within Escherichia coli cells. (c) 2015 Elsevier B.V. All rights reserved.
T2 - Colloids and Surfaces. B: Biointerfaces
T1 - Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells
VL - 135
SP - 742
EP - 750
DO - 10.1016/j.colsurfb.2015.08.050
ER -
@article{
author = "Pajović, Jelena D. and Dojčilović, Radovan and Božanić, Dušan K. and Kascakova, Slavka and Refregiers, Matthieu and Dimitrijević-Branković, Suzana I. and Vodnik, Vesna and Milosavljević, Aleksandar R. and Piscopiello, Emanuela and Luyt, Adriaan S. and Đoković, Vladimir",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/875",
abstract = "Biocompatible fluorescent nanostructures were prepared by a functionalization of gold nanoparticles with the amino acid tryptophan. The gold-tryptophan bioconjugates were investigated by TEM and HRTEM and various spectroscopy methods (XPS, FTIR, UV-vis and photoluminescence). It was found that the gold nanoparticles, initially 8 nm in diameter, aggregate in the presence of the amino acid. From the XPS and FTIR spectroscopy results, it was concluded that the tryptophan gold interactions mainly take place via indole and carboxyl groups. Although the indole group is involved in the interaction with the gold surfaces, the tryptophan-gold hybrids showed strong fluorescence due to the presence of multilayers of tryptophan. Deep ultra violet (DUV) imaging performed at the SOLEIL synchrotron showed that It is possible to detect these hybrid nanostructures within Escherichia coli cells. (c) 2015 Elsevier B.V. All rights reserved.",
journal = "Colloids and Surfaces. B: Biointerfaces",
title = "Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells",
volume = "135",
pages = "742-750",
doi = "10.1016/j.colsurfb.2015.08.050"
}
Pajović JD, Dojčilović R, Božanić DK, Kascakova S, Refregiers M, Dimitrijević-Branković SI, Vodnik V, Milosavljević AR, Piscopiello E, Luyt AS, Đoković V. Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells. Colloids and Surfaces. B: Biointerfaces. 2015;135:742-750
Pajović, J. D., Dojčilović, R., Božanić, D. K., Kascakova, S., Refregiers, M., Dimitrijević-Branković, S. I., Vodnik, V., Milosavljević, A. R., Piscopiello, E., Luyt, A. S.,& Đoković, V. (2015). Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells.
Colloids and Surfaces. B: Biointerfaces, 135, 742-750.
https://doi.org/10.1016/j.colsurfb.2015.08.050
Pajović Jelena D., Dojčilović Radovan, Božanić Dušan K., Kascakova Slavka, Refregiers Matthieu, Dimitrijević-Branković Suzana I., Vodnik Vesna, Milosavljević Aleksandar R., Piscopiello Emanuela, Luyt Adriaan S., Đoković Vladimir, "Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells" 135 (2015):742-750,
https://doi.org/10.1016/j.colsurfb.2015.08.050 .
