In vitro biocompatibility assessment of Co-Cr-Mo dental cast alloy
Putić, Slaviša S.
Rakin, Marko P.
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Metallic materials, such as Co-Cr-Mo alloys, are exposed to aggressive conditions in the oral cavity that represents an ideal environment for metallic ion release and biodegradation. The metallic ions released from dental materials can cause local and/or systemic adverse effects in the human body. Therefore, dental materials are required to possess appropriate mechanical, physical, chemical and biological properties. The biocompatibility of metallic materials is very important for dental applications. Accordingly, the aim of this study was to examine metallic ion release and cytotoxicity of Co-30Cr-5Mo cast alloy as the initial phase of biocompatibility evaluation. Determination of the viability of human (MRC-5) and animal (L929) fibroblast cells were conducted using three in vitro test methods: the colorimetric methyl-thiazol-tetrazolium (MTT) test, the dye exclusion test (DET) and the agar diffusion test (ADT). Furthermore, the morphology and growth of the cells were analyzed using s...canning electron microscopy (SEM). The obtained results indicated that Co-30Cr-5Mo alloy did not release harmful elements in concentrations high enough to have detrimental effects on human and animal fibroblasts under the given experimental conditions. Moreover, the fibroblast cells showed good adhesion on the surface of the Co-30Cr-5Mo alloy. Therefore, it could be concluded that Co-30Cr-5Mo alloy is a biocompatible material that could be safely used in dentistry.