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dc.creatorLaban, Bojana B.
dc.creatorRalević, Uroš
dc.creatorPetrović, Sandra
dc.creatorLeskovac, Andreja
dc.creatorVasić Anićijević, Dragana D.
dc.creatorMarković, Mirjana
dc.creatorVasić, Vesna M.
dc.date.accessioned2020-06-05T13:25:44Z
dc.date.available2020-06-05T13:25:44Z
dc.date.issued2020
dc.identifier.issn0162-0134
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/8673
dc.description.abstractThe simple green method for synthesis of stable L-Methionine (L-Met) capped silver (Ag@LM NPs) and gold (Au@LM NPs) nanoparticles (NPs) without adding any additional reduction agent or stabilizer was developed. Colloidal dispersions were characterized by UV–Vis spectrophotometry. The size and spherical shape of NPs were evaluated by transmission electron microscopy. Their surface covering was confirmed by atomic force microscopy, Fourier transform infrared spectroscopy, dynamic light scattering, and zeta potential measurements. Density functional theory calculations pointed that the preferential adsorption mode of L-Met on both Ag and Au surfaces was a vertical binding geometry via –NH2 group, while horizontal binding mode via [sbnd]S[sbnd] and –NH2 groups is also possible. The genotoxicity (evaluated by the micronucleus assay) of NPs, as well as their effects on some oxidative stress parameters (catalase activity, malondialdehyde level), were assessed in vitro using human peripheral blood cells as a model system. The influence of NPs on the morphology of lymphocyte cells studied using atomic force microscopy revealed that the membrane of cells remained unaffected after the treatment with NPs. When considering the effects of NPs on catalase activity and malondialdehyde level, neither particle type promoted oxidative stress. However, the treatment of lymphocytes with Ag@LM NPs induced a concentration-dependent enhancement of the micronuclei incidence and suppression of the cell proliferation while Au@LM NPs promoted cell proliferation, with no significant effects on micronuclei formation. The Ag@LM NPs were more prone to induce DNA damage than Au@LM NPs, which makes the latter type more suitable for further studies in nano-medicine. © 2019en
dc.language.isoen
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172023/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172045/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Inorganic Biochemistry
dc.subjectSilveren
dc.subjectGolden
dc.subjectGreenen
dc.subjectNanoparticlesen
dc.subjectL-methionineen
dc.subjectToxicityen
dc.titleGreen synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticlesen
dc.typearticleen
dc.rights.licenseARR
dcterms.abstractРалевић, У; Лесковац, A; Васић-Aнићијевић, Д; Марковић, М; Васић, В; Петровић, С; Лабан, Б;
dc.rights.holder© 2019
dc.citation.volume204
dc.citation.spage110958
dc.identifier.wos000528628200012
dc.identifier.doi10.1016/j.jinorgbio.2019.110958
dc.citation.rankM21~
dc.identifier.pmid31841761
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-85076247598


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