Show simple item record

dc.creatorKorićanac, Lela
dc.creatorŽakula, Jelena
dc.creatorCirrone, Giuseppe Antonio Pablo
dc.creatorPrivitera, Giuseppe
dc.creatorCuttone, Giacomo
dc.creatorPetrović, Ivan M.
dc.creatorRistić-Fira, Aleksandra
dc.date.accessioned2020-05-25T10:27:39Z
dc.date.available2020-05-25T10:27:39Z
dc.date.issued2012
dc.identifier.issn1936-6612
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/8644
dc.description.abstractThe effects of combined treatments with fotemustine and proton radiation on cell proliferation and induction of apoptosis have been analyzed in this study. HTB140 human melanoma cells were treated with fotemustine (100, 250 M) 24 h prior to irradiation (12, 16 Gy). The cells were irradiated in the middle of a therapeutic 62 MeV proton spread-out Bragg peak. An efficiency of applied treatments was observed throughout the evaluation of the cell proliferation 7 days after proton irradiation. The combined treatments with fotemustine and protons resulted in a greater antiproliferative response than each treatment alone. The number of apoptotic cells was estimated after 6 or 48 h using flow cytometry. The highest percentage of apoptotic cells was obtained 48 h after treatment with 250 M fotemustine and protons. Western blot analysis showed that induction of apoptosis was associated with p53 and Bax up regulation, and Bcl-2 down regulation. The induction of a caspase-3 activity and cleavage of PARP were clearly observed. These data indicate that a combined application of FM and proton irradiation is more effective in reducing melanoma cell proliferation and the induction of apoptosis, suggesting that FM can increase the radio-sensitivity of HTB140 melanoma cells.en
dc.language.isoen
dc.rightsrestrictedAccess
dc.sourceAdvanced Science Letters
dc.subjectapoptotic cellsen
dc.subjectfotemustineen
dc.subjecthuman melanoma cellsen
dc.subjectproton radiationen
dc.titleVariation of Apoptotic Pathway Regulators by Fotemustine and Protons in a Human Melanoma Cell Lineen
dc.typearticleen
dc.rights.licenseARR
dcterms.abstractКорићанац, Лела; Ристић-Фира, Aлександра; Петровић, Иван М.; Цуттоне, Гиацомо; Привитера, Гиусеппе; Цирроне, Гиусеппе Aнтонио Пабло; Жакула, Јелена;
dc.citation.volume5
dc.citation.issue2
dc.citation.spage552
dc.citation.epage559
dc.identifier.doi10.1166/asl.2012.2150
dc.citation.rankM21
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-84880822395


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record