Protective effects of whey on rat liver damage induced by chronic alcohol intake
Authorized Users Only
2019
Authors
Radić, IvanMijović, Milica
Tatalović, Nikola R.
Mitić, Miloš
Lukić, Vera
Joksimović, Bojan
Petrović, Zorica
Ristić, Siniša
Veličković, Stefan
Nestorović, Vojkan
Ćorac, Aleksandar M.
Mirić, Mirjana
Adžić, Miroslav
Blagojević, Duško P.
Popović, Ljiljana M.
Janićijević-Hudomal, Snežana
Article (Published version)
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© The Author(s) 2019
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In 2012, alcohol liver disease resulted in 3.3 million—5.9% of global deaths. This study introduced whey protection capacity against chronic alcohol-induced liver injury. Rats were orally administered to 12% ethanol solution in water (ad libitum, average 8.14 g of ethanol/kg body weight (b.w.)/day) alone or combined with whey (per os, 2 g/kg b.w./day). After 6-week treatment, chronic ethanol consumption induced significant histopathological liver changes: congestion, central vein dilation, hepatic portal vein branch dilation, Kupffer cells hyperplasia, fatty liver changes, and hepatocytes focal necrosis. Ethanol significantly increased liver catalase activity and glutathione reductase protein expression without significant effects on antioxidative enzymes: glutathione peroxidase (GPx), copper–zinc-containing superoxide dismutase (CuZnSOD) and manganese-containing superoxide dismutase (MnSOD). Co-treatment with whey significantly attenuated pathohistological changes induced by ethanol i...ngestion and increased GSH-Px and nuclear factor kappa B (NF-κB) protein expression. Our results showed positive effects of whey on liver chronically exposed to ethanol, which seem to be associated with NF-κB-GPx signaling. © The Author(s) 2019.
Keywords:
Alcohol liver disease / rat / whey / ethanol / antioxidant enzymes / NF-BSource:
Human and Experimental Toxicology, 2019, 38, 6, 632-645Funding / projects:
- Molecular mechanisms of redox signalling in homeostasis: adaptation and pathology (RS-MESTD-Basic Research (BR or ON)-173014)
- Defining a cluster of molecular biomarkers for improved diagnostics and therapy of mood disorders (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41029)
DOI: 10.1177/0960327119829518
ISSN: 0960-3271
PubMed: 30784321
WoS: 000470714400002
Scopus: 2-s2.0-85062018247
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VinčaTY - JOUR AU - Radić, Ivan AU - Mijović, Milica AU - Tatalović, Nikola R. AU - Mitić, Miloš AU - Lukić, Vera AU - Joksimović, Bojan AU - Petrović, Zorica AU - Ristić, Siniša AU - Veličković, Stefan AU - Nestorović, Vojkan AU - Ćorac, Aleksandar M. AU - Mirić, Mirjana AU - Adžić, Miroslav AU - Blagojević, Duško P. AU - Popović, Ljiljana M. AU - Janićijević-Hudomal, Snežana PY - 2019 UR - https://vinar.vin.bg.ac.rs/handle/123456789/8541 AB - In 2012, alcohol liver disease resulted in 3.3 million—5.9% of global deaths. This study introduced whey protection capacity against chronic alcohol-induced liver injury. Rats were orally administered to 12% ethanol solution in water (ad libitum, average 8.14 g of ethanol/kg body weight (b.w.)/day) alone or combined with whey (per os, 2 g/kg b.w./day). After 6-week treatment, chronic ethanol consumption induced significant histopathological liver changes: congestion, central vein dilation, hepatic portal vein branch dilation, Kupffer cells hyperplasia, fatty liver changes, and hepatocytes focal necrosis. Ethanol significantly increased liver catalase activity and glutathione reductase protein expression without significant effects on antioxidative enzymes: glutathione peroxidase (GPx), copper–zinc-containing superoxide dismutase (CuZnSOD) and manganese-containing superoxide dismutase (MnSOD). Co-treatment with whey significantly attenuated pathohistological changes induced by ethanol ingestion and increased GSH-Px and nuclear factor kappa B (NF-κB) protein expression. Our results showed positive effects of whey on liver chronically exposed to ethanol, which seem to be associated with NF-κB-GPx signaling. © The Author(s) 2019. T2 - Human and Experimental Toxicology T1 - Protective effects of whey on rat liver damage induced by chronic alcohol intake VL - 38 IS - 6 SP - 632 EP - 645 DO - 10.1177/0960327119829518 ER -
@article{ author = "Radić, Ivan and Mijović, Milica and Tatalović, Nikola R. and Mitić, Miloš and Lukić, Vera and Joksimović, Bojan and Petrović, Zorica and Ristić, Siniša and Veličković, Stefan and Nestorović, Vojkan and Ćorac, Aleksandar M. and Mirić, Mirjana and Adžić, Miroslav and Blagojević, Duško P. and Popović, Ljiljana M. and Janićijević-Hudomal, Snežana", year = "2019", abstract = "In 2012, alcohol liver disease resulted in 3.3 million—5.9% of global deaths. This study introduced whey protection capacity against chronic alcohol-induced liver injury. Rats were orally administered to 12% ethanol solution in water (ad libitum, average 8.14 g of ethanol/kg body weight (b.w.)/day) alone or combined with whey (per os, 2 g/kg b.w./day). After 6-week treatment, chronic ethanol consumption induced significant histopathological liver changes: congestion, central vein dilation, hepatic portal vein branch dilation, Kupffer cells hyperplasia, fatty liver changes, and hepatocytes focal necrosis. Ethanol significantly increased liver catalase activity and glutathione reductase protein expression without significant effects on antioxidative enzymes: glutathione peroxidase (GPx), copper–zinc-containing superoxide dismutase (CuZnSOD) and manganese-containing superoxide dismutase (MnSOD). Co-treatment with whey significantly attenuated pathohistological changes induced by ethanol ingestion and increased GSH-Px and nuclear factor kappa B (NF-κB) protein expression. Our results showed positive effects of whey on liver chronically exposed to ethanol, which seem to be associated with NF-κB-GPx signaling. © The Author(s) 2019.", journal = "Human and Experimental Toxicology", title = "Protective effects of whey on rat liver damage induced by chronic alcohol intake", volume = "38", number = "6", pages = "632-645", doi = "10.1177/0960327119829518" }
Radić, I., Mijović, M., Tatalović, N. R., Mitić, M., Lukić, V., Joksimović, B., Petrović, Z., Ristić, S., Veličković, S., Nestorović, V., Ćorac, A. M., Mirić, M., Adžić, M., Blagojević, D. P., Popović, L. M.,& Janićijević-Hudomal, S.. (2019). Protective effects of whey on rat liver damage induced by chronic alcohol intake. in Human and Experimental Toxicology, 38(6), 632-645. https://doi.org/10.1177/0960327119829518
Radić I, Mijović M, Tatalović NR, Mitić M, Lukić V, Joksimović B, Petrović Z, Ristić S, Veličković S, Nestorović V, Ćorac AM, Mirić M, Adžić M, Blagojević DP, Popović LM, Janićijević-Hudomal S. Protective effects of whey on rat liver damage induced by chronic alcohol intake. in Human and Experimental Toxicology. 2019;38(6):632-645. doi:10.1177/0960327119829518 .
Radić, Ivan, Mijović, Milica, Tatalović, Nikola R., Mitić, Miloš, Lukić, Vera, Joksimović, Bojan, Petrović, Zorica, Ristić, Siniša, Veličković, Stefan, Nestorović, Vojkan, Ćorac, Aleksandar M., Mirić, Mirjana, Adžić, Miroslav, Blagojević, Duško P., Popović, Ljiljana M., Janićijević-Hudomal, Snežana, "Protective effects of whey on rat liver damage induced by chronic alcohol intake" in Human and Experimental Toxicology, 38, no. 6 (2019):632-645, https://doi.org/10.1177/0960327119829518 . .