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dc.creatorMilojkov, Dušan V.
dc.creatorSilvestre, Oscar F.
dc.creatorStanić, Vojislav
dc.creatorJanjić, Goran V.
dc.creatorMutavdžić, Dragosav R.
dc.creatorMilanović, Marija M.
dc.creatorNieder, Jana B.
dc.date.accessioned2020-03-30T16:24:39Z
dc.date.available2020-03-30T16:24:39Z
dc.date.issued2020
dc.identifier.issn0022-2313
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/8529
dc.description.abstractFluorapatite doped with rare-earth elements has a wide-range of biomedical applications. Here, a new type of fluorapatite nanocrystals doped with praseodymium (FAP-Pr) with excitation-emission profiles in visible part of the spectrum is fabricated. Energy levels of Pr3+ activator ion contain metastable multiplet states that offer the possibility of efficient multicolor emission lines in FAP nanocrystals. Three types of FAP-Pr nanocrystals with 0.1%, 0.5% and 1% atomic percent of Pr3+ (along with the undoped FAP control sample) are studied. Their novel chemical production method is described, the FAP-Pr nanocrystals structure, biocompatibility and the suitability for cell imaging are analyzed. Physicochemical characterization confirms crystals down to nanometer size. In addition, quantum-chemical calculation predicts that Pr3+ ions are incorporated into the FAP crystal lattice at Ca2 (6 h) sites. In vitro viability results shows that FAP-Pr nanocrystals are nontoxic to live cells. Additionally, the cell uptake of the FAP-Pr nanocrystals is studied using fluorescence-based widefield and confocal microscopy. The nanocrystals show characteristic green emission at 545 nm (3P0→3H5 transition of Pr3+ ion) and orange emission at 600 nm (1D2→3H4), which we use to discriminate from cell autofluorescence background. Orthogonal projections across 3D confocal stacks show that the nanocrystals are able to enter the cells positioning themselves within the cytoplasm. Overall, the new FAP-Pr nanocrystals are biocompatible and of the tested types, the 0.5% Pr3+ doped nanocrystals show the highest promise as a tracking nanoparticle probe for bioimaging applications. © 2019en
dc.language.isoen
dc.relationCOST Action [MP1302]
dc.relationCCDR-N [NORTE-01-0145-FEDER-000019]
dc.relationEuropean Union (EU) [600375]
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43009/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45021/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172023/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173017/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Luminescence
dc.subjectFluorapatite nanocrystalsen
dc.subjectPraseodymiumen
dc.subjectBioimagingen
dc.subjectCancer cellsen
dc.titleFabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agentsen
dc.typearticleen
dc.rights.licenseARR
dcterms.abstractМилановић, Марија; Ниедер, Јана Б; Милојков, Душан В; Силвестре, Осцар Ф; Станић, Војислав Ђ.; Јањић, Горан В; Мутавджић, Драгосав Р;
dc.rights.holder© 2019 Published by Elsevier B.V.
dc.citation.volume217
dc.citation.spage116757
dc.identifier.wos000501362400035
dc.identifier.doi10.1016/j.jlumin.2019.116757
dc.citation.rankM21~
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-85073162138


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