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Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions

Authorized Users Only
2019
Authors
Davidović, Slađana Z.
Lazić, Vesna M.
Miljković, Miona G.
Gordić, Milan V.
Sekulić, Milica
Marinović-Cincović, Milena
Ratnayake, Ishara S.
Ahrenkiel, Scott Phillip
Nedeljković, Jovan
Article (Published version)
,
© 2019 Elsevier Ltd
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Abstract
The antibacterial ability of in situ prepared nanometer-sized silver particles, immobilized in agar-agar films, was studied as a function of the concentration of co-dopant, magnesium ions. Content of inorganic components in hybrid films was determined using inductively coupled plasma optic emission spectroscopy, and found to be low (<2 wt.-%). Morphology of prepared hybrid films, studied by transmission electron microscopy, revealed the presence of non-agglomerated and randomly distributed 10–20 nm silver nanoparticles (Ag NPs) within the agar-agar matrices. Fourier-transform infrared spectroscopy indicated the distinct chemical interaction between Ag NPs and polymer chains. Thermogravimetric analysis, as well as the determination of tensile strength, Young's modulus, and elongation at break showed improvement of thermal stability and mechanical properties of agar-agar matrices upon the incorporation of Ag NPs due to high compatibility between the hydrophilic organic component and inor...ganic components. The complete microbial reduction of Gram-positive bacteria Staphylococcus aureuswas observed for all agar-silver films, while satisfactory results were observed for Gram-negative bacteria Pseudomonas aeruginosa (≥99.6%). The release of Ag+ ions is suppressed by the increase of the concentration of Mg2+ ions and it was found to be significantly smaller (≤0.24 ppm) than the harmful ecological level (1 ppm). © 2019 Elsevier Ltd

Keywords:
Agar-agar / Silver nanoparticles / Nanocomposite films / Antimicrobial activity
Source:
Carbohydrate Polymers, 2019, 224, 115187-
Funding / projects:
  • Materials of Reduced Dimensions for Efficient Light Harvesting and Energy conversion (RS-45020)
  • Application of biotechnological methods for sustainable exploitation of by-products of agro-industry (RS-31035)

DOI: 10.1016/j.carbpol.2019.115187

ISSN: 0144-8617

PubMed: 31472840

WoS: 000484001200034

Scopus: 2-s2.0-85070631458
[ Google Scholar ]
18
8
URI
https://vinar.vin.bg.ac.rs/handle/123456789/8452
Collections
  • Radovi istraživača
Institution/Community
Vinča
TY  - JOUR
AU  - Davidović, Slađana Z.
AU  - Lazić, Vesna M.
AU  - Miljković, Miona G.
AU  - Gordić, Milan V.
AU  - Sekulić, Milica
AU  - Marinović-Cincović, Milena
AU  - Ratnayake, Ishara S.
AU  - Ahrenkiel, Scott Phillip
AU  - Nedeljković, Jovan
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8452
AB  - The antibacterial ability of in situ prepared nanometer-sized silver particles, immobilized in agar-agar films, was studied as a function of the concentration of co-dopant, magnesium ions. Content of inorganic components in hybrid films was determined using inductively coupled plasma optic emission spectroscopy, and found to be low (<2 wt.-%). Morphology of prepared hybrid films, studied by transmission electron microscopy, revealed the presence of non-agglomerated and randomly distributed 10–20 nm silver nanoparticles (Ag NPs) within the agar-agar matrices. Fourier-transform infrared spectroscopy indicated the distinct chemical interaction between Ag NPs and polymer chains. Thermogravimetric analysis, as well as the determination of tensile strength, Young's modulus, and elongation at break showed improvement of thermal stability and mechanical properties of agar-agar matrices upon the incorporation of Ag NPs due to high compatibility between the hydrophilic organic component and inorganic components. The complete microbial reduction of Gram-positive bacteria Staphylococcus aureuswas observed for all agar-silver films, while satisfactory results were observed for Gram-negative bacteria Pseudomonas aeruginosa (≥99.6%). The release of Ag+ ions is suppressed by the increase of the concentration of Mg2+ ions and it was found to be significantly smaller (≤0.24 ppm) than the harmful ecological level (1 ppm). © 2019 Elsevier Ltd
T2  - Carbohydrate Polymers
T1  - Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions
VL  - 224
SP  - 115187
DO  - 10.1016/j.carbpol.2019.115187
ER  - 
@article{
author = "Davidović, Slađana Z. and Lazić, Vesna M. and Miljković, Miona G. and Gordić, Milan V. and Sekulić, Milica and Marinović-Cincović, Milena and Ratnayake, Ishara S. and Ahrenkiel, Scott Phillip and Nedeljković, Jovan",
year = "2019",
abstract = "The antibacterial ability of in situ prepared nanometer-sized silver particles, immobilized in agar-agar films, was studied as a function of the concentration of co-dopant, magnesium ions. Content of inorganic components in hybrid films was determined using inductively coupled plasma optic emission spectroscopy, and found to be low (<2 wt.-%). Morphology of prepared hybrid films, studied by transmission electron microscopy, revealed the presence of non-agglomerated and randomly distributed 10–20 nm silver nanoparticles (Ag NPs) within the agar-agar matrices. Fourier-transform infrared spectroscopy indicated the distinct chemical interaction between Ag NPs and polymer chains. Thermogravimetric analysis, as well as the determination of tensile strength, Young's modulus, and elongation at break showed improvement of thermal stability and mechanical properties of agar-agar matrices upon the incorporation of Ag NPs due to high compatibility between the hydrophilic organic component and inorganic components. The complete microbial reduction of Gram-positive bacteria Staphylococcus aureuswas observed for all agar-silver films, while satisfactory results were observed for Gram-negative bacteria Pseudomonas aeruginosa (≥99.6%). The release of Ag+ ions is suppressed by the increase of the concentration of Mg2+ ions and it was found to be significantly smaller (≤0.24 ppm) than the harmful ecological level (1 ppm). © 2019 Elsevier Ltd",
journal = "Carbohydrate Polymers",
title = "Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions",
volume = "224",
pages = "115187",
doi = "10.1016/j.carbpol.2019.115187"
}
Davidović, S. Z., Lazić, V. M., Miljković, M. G., Gordić, M. V., Sekulić, M., Marinović-Cincović, M., Ratnayake, I. S., Ahrenkiel, S. P.,& Nedeljković, J.. (2019). Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions. in Carbohydrate Polymers, 224, 115187.
https://doi.org/10.1016/j.carbpol.2019.115187
Davidović SZ, Lazić VM, Miljković MG, Gordić MV, Sekulić M, Marinović-Cincović M, Ratnayake IS, Ahrenkiel SP, Nedeljković J. Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions. in Carbohydrate Polymers. 2019;224:115187.
doi:10.1016/j.carbpol.2019.115187 .
Davidović, Slađana Z., Lazić, Vesna M., Miljković, Miona G., Gordić, Milan V., Sekulić, Milica, Marinović-Cincović, Milena, Ratnayake, Ishara S., Ahrenkiel, Scott Phillip, Nedeljković, Jovan, "Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions" in Carbohydrate Polymers, 224 (2019):115187,
https://doi.org/10.1016/j.carbpol.2019.115187 . .

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