Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions
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2019
Authors
Davidović, Slađana Z.
Lazić, Vesna M.

Miljković, Miona G.

Gordić, Milan V.

Sekulić, Milica

Marinović-Cincović, Milena

Ratnayake, Ishara S.
Ahrenkiel, Scott Phillip

Nedeljković, Jovan

Article (Published version)

© 2019 Elsevier Ltd
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The antibacterial ability of in situ prepared nanometer-sized silver particles, immobilized in agar-agar films, was studied as a function of the concentration of co-dopant, magnesium ions. Content of inorganic components in hybrid films was determined using inductively coupled plasma optic emission spectroscopy, and found to be low (<2 wt.-%). Morphology of prepared hybrid films, studied by transmission electron microscopy, revealed the presence of non-agglomerated and randomly distributed 10–20 nm silver nanoparticles (Ag NPs) within the agar-agar matrices. Fourier-transform infrared spectroscopy indicated the distinct chemical interaction between Ag NPs and polymer chains. Thermogravimetric analysis, as well as the determination of tensile strength, Young's modulus, and elongation at break showed improvement of thermal stability and mechanical properties of agar-agar matrices upon the incorporation of Ag NPs due to high compatibility between the hydrophilic organic component and inor...ganic components. The complete microbial reduction of Gram-positive bacteria Staphylococcus aureuswas observed for all agar-silver films, while satisfactory results were observed for Gram-negative bacteria Pseudomonas aeruginosa (≥99.6%). The release of Ag+ ions is suppressed by the increase of the concentration of Mg2+ ions and it was found to be significantly smaller (≤0.24 ppm) than the harmful ecological level (1 ppm). © 2019 Elsevier Ltd
Keywords:
Agar-agar / Silver nanoparticles / Nanocomposite films / Antimicrobial activitySource:
Carbohydrate Polymers, 2019, 224, 115187-Funding / projects:
- Materials of Reduced Dimensions for Efficient Light Harvesting and Energy conversion (RS-45020)
- Application of biotechnological methods for sustainable exploitation of by-products of agro-industry (RS-31035)
DOI: 10.1016/j.carbpol.2019.115187
ISSN: 0144-8617
PubMed: 31472840
WoS: 000484001200034
Scopus: 2-s2.0-85070631458
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VinčaTY - JOUR AU - Davidović, Slađana Z. AU - Lazić, Vesna M. AU - Miljković, Miona G. AU - Gordić, Milan V. AU - Sekulić, Milica AU - Marinović-Cincović, Milena AU - Ratnayake, Ishara S. AU - Ahrenkiel, Scott Phillip AU - Nedeljković, Jovan PY - 2019 UR - https://vinar.vin.bg.ac.rs/handle/123456789/8452 AB - The antibacterial ability of in situ prepared nanometer-sized silver particles, immobilized in agar-agar films, was studied as a function of the concentration of co-dopant, magnesium ions. Content of inorganic components in hybrid films was determined using inductively coupled plasma optic emission spectroscopy, and found to be low (<2 wt.-%). Morphology of prepared hybrid films, studied by transmission electron microscopy, revealed the presence of non-agglomerated and randomly distributed 10–20 nm silver nanoparticles (Ag NPs) within the agar-agar matrices. Fourier-transform infrared spectroscopy indicated the distinct chemical interaction between Ag NPs and polymer chains. Thermogravimetric analysis, as well as the determination of tensile strength, Young's modulus, and elongation at break showed improvement of thermal stability and mechanical properties of agar-agar matrices upon the incorporation of Ag NPs due to high compatibility between the hydrophilic organic component and inorganic components. The complete microbial reduction of Gram-positive bacteria Staphylococcus aureuswas observed for all agar-silver films, while satisfactory results were observed for Gram-negative bacteria Pseudomonas aeruginosa (≥99.6%). The release of Ag+ ions is suppressed by the increase of the concentration of Mg2+ ions and it was found to be significantly smaller (≤0.24 ppm) than the harmful ecological level (1 ppm). © 2019 Elsevier Ltd T2 - Carbohydrate Polymers T1 - Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions VL - 224 SP - 115187 DO - 10.1016/j.carbpol.2019.115187 ER -
@article{ author = "Davidović, Slađana Z. and Lazić, Vesna M. and Miljković, Miona G. and Gordić, Milan V. and Sekulić, Milica and Marinović-Cincović, Milena and Ratnayake, Ishara S. and Ahrenkiel, Scott Phillip and Nedeljković, Jovan", year = "2019", abstract = "The antibacterial ability of in situ prepared nanometer-sized silver particles, immobilized in agar-agar films, was studied as a function of the concentration of co-dopant, magnesium ions. Content of inorganic components in hybrid films was determined using inductively coupled plasma optic emission spectroscopy, and found to be low (<2 wt.-%). Morphology of prepared hybrid films, studied by transmission electron microscopy, revealed the presence of non-agglomerated and randomly distributed 10–20 nm silver nanoparticles (Ag NPs) within the agar-agar matrices. Fourier-transform infrared spectroscopy indicated the distinct chemical interaction between Ag NPs and polymer chains. Thermogravimetric analysis, as well as the determination of tensile strength, Young's modulus, and elongation at break showed improvement of thermal stability and mechanical properties of agar-agar matrices upon the incorporation of Ag NPs due to high compatibility between the hydrophilic organic component and inorganic components. The complete microbial reduction of Gram-positive bacteria Staphylococcus aureuswas observed for all agar-silver films, while satisfactory results were observed for Gram-negative bacteria Pseudomonas aeruginosa (≥99.6%). The release of Ag+ ions is suppressed by the increase of the concentration of Mg2+ ions and it was found to be significantly smaller (≤0.24 ppm) than the harmful ecological level (1 ppm). © 2019 Elsevier Ltd", journal = "Carbohydrate Polymers", title = "Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions", volume = "224", pages = "115187", doi = "10.1016/j.carbpol.2019.115187" }
Davidović, S. Z., Lazić, V. M., Miljković, M. G., Gordić, M. V., Sekulić, M., Marinović-Cincović, M., Ratnayake, I. S., Ahrenkiel, S. P.,& Nedeljković, J.. (2019). Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions. in Carbohydrate Polymers, 224, 115187. https://doi.org/10.1016/j.carbpol.2019.115187
Davidović SZ, Lazić VM, Miljković MG, Gordić MV, Sekulić M, Marinović-Cincović M, Ratnayake IS, Ahrenkiel SP, Nedeljković J. Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions. in Carbohydrate Polymers. 2019;224:115187. doi:10.1016/j.carbpol.2019.115187 .
Davidović, Slađana Z., Lazić, Vesna M., Miljković, Miona G., Gordić, Milan V., Sekulić, Milica, Marinović-Cincović, Milena, Ratnayake, Ishara S., Ahrenkiel, Scott Phillip, Nedeljković, Jovan, "Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions" in Carbohydrate Polymers, 224 (2019):115187, https://doi.org/10.1016/j.carbpol.2019.115187 . .