Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study
Samo za registrovane korisnike
2019
Autori
Kopčalić, KatarinaPetrović, Nina
Stanojković, Tatjana P.
Stanković, Vesna
Bukumirić, Zoran
Roganović, Jelena
Mališić, Emina
Nikitović, Marina
Članak u časopisu (Objavljena verzija)
,
© 2018 Elsevier GmbH
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Introduction: Nearly sixty percent of patients with prostate cancer (PCa) undergo radiation therapy (RT). During the course of treatment patients may experience normal tissue reactions. It is a well established fact that genetic and epigenetic mechanisms, such as microRNA (miRNA) level changes might be associated with radiotoxicity, as a response to irradiation. Materials and methods: This is the first study that has investigated levels of radiosensory miRNAs in association with acute genitourinary radiotoxicity extracted from peripheral blood mononuclear cells (PBCs), in three points; before RT (BRT), after RT (ART) and on the first control examination (FCONT). We measured levels of miR-21/146a/155 expression by quantitative real-time PCR (qRT-PCR), comparative ΔΔCt method, in fifteen patients with localized prostate cancer, treated with three-dimensional conformal radiotherapy (3DCRT). Nine subjects have experienced acute genitourinary (GU) radiotoxicity whereas six where without GU ...radiotoxicity. Results: Firstly, we detected the highest levels of miR-21 in ART group (p = 0.043) in the patients with acute GU radiotoxicity. Secondly, we found trend towards higher miR-21 levels and significantly higher levels of miR-146a/155 within the patients with acute GU toxicity than in patients without (p = 0.068, p = 0.016, and p = 0.010, respectively). Thirdly, we detected significant change in miR-146a/155 levels within the patients without acute GU radiotoxicity during RT p = 0.042, and p = 0.041, respectively). Conclusion: miR-21/146a/155 might be useful potential factors of radiosensitivity and acute genitourinary radiotoxicity in prostate cancer patients. miRNA might have great potential as predictors of various pathological conditions extracted from PBMCs. © 2018 Elsevier GmbH
Ključne reči:
Prostate cancer / Conformal radiotherapy / Acute genitourinary radiotoxicity / miR-21 / miR-146a / miR-155Izvor:
Pathology - Research and Practice, 2019, 215, 4, 626-631Finansiranje / projekti:
- Modifikatori biološkog odgovora u fiziološkim i patološkim stanjima (RS-MESTD-Basic Research (BR or ON)-175011)
- Kontrola bola i molekularni mehanizmi kao faktori regenerativne terapije u stomatologiji kod zdravih i pacijenata sa dijabetes melitusom (RS-MESTD-Basic Research (BR or ON)-175021)
DOI: 10.1016/j.prp.2018.12.007
ISSN: 0344-0338
PubMed: 30554863
WoS: 000474319300002
Scopus: 2-s2.0-85058219558
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Kopčalić, Katarina AU - Petrović, Nina AU - Stanojković, Tatjana P. AU - Stanković, Vesna AU - Bukumirić, Zoran AU - Roganović, Jelena AU - Mališić, Emina AU - Nikitović, Marina PY - 2019 UR - https://vinar.vin.bg.ac.rs/handle/123456789/8444 AB - Introduction: Nearly sixty percent of patients with prostate cancer (PCa) undergo radiation therapy (RT). During the course of treatment patients may experience normal tissue reactions. It is a well established fact that genetic and epigenetic mechanisms, such as microRNA (miRNA) level changes might be associated with radiotoxicity, as a response to irradiation. Materials and methods: This is the first study that has investigated levels of radiosensory miRNAs in association with acute genitourinary radiotoxicity extracted from peripheral blood mononuclear cells (PBCs), in three points; before RT (BRT), after RT (ART) and on the first control examination (FCONT). We measured levels of miR-21/146a/155 expression by quantitative real-time PCR (qRT-PCR), comparative ΔΔCt method, in fifteen patients with localized prostate cancer, treated with three-dimensional conformal radiotherapy (3DCRT). Nine subjects have experienced acute genitourinary (GU) radiotoxicity whereas six where without GU radiotoxicity. Results: Firstly, we detected the highest levels of miR-21 in ART group (p = 0.043) in the patients with acute GU radiotoxicity. Secondly, we found trend towards higher miR-21 levels and significantly higher levels of miR-146a/155 within the patients with acute GU toxicity than in patients without (p = 0.068, p = 0.016, and p = 0.010, respectively). Thirdly, we detected significant change in miR-146a/155 levels within the patients without acute GU radiotoxicity during RT p = 0.042, and p = 0.041, respectively). Conclusion: miR-21/146a/155 might be useful potential factors of radiosensitivity and acute genitourinary radiotoxicity in prostate cancer patients. miRNA might have great potential as predictors of various pathological conditions extracted from PBMCs. © 2018 Elsevier GmbH T2 - Pathology - Research and Practice T1 - Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study VL - 215 IS - 4 SP - 626 EP - 631 DO - 10.1016/j.prp.2018.12.007 ER -
@article{ author = "Kopčalić, Katarina and Petrović, Nina and Stanojković, Tatjana P. and Stanković, Vesna and Bukumirić, Zoran and Roganović, Jelena and Mališić, Emina and Nikitović, Marina", year = "2019", abstract = "Introduction: Nearly sixty percent of patients with prostate cancer (PCa) undergo radiation therapy (RT). During the course of treatment patients may experience normal tissue reactions. It is a well established fact that genetic and epigenetic mechanisms, such as microRNA (miRNA) level changes might be associated with radiotoxicity, as a response to irradiation. Materials and methods: This is the first study that has investigated levels of radiosensory miRNAs in association with acute genitourinary radiotoxicity extracted from peripheral blood mononuclear cells (PBCs), in three points; before RT (BRT), after RT (ART) and on the first control examination (FCONT). We measured levels of miR-21/146a/155 expression by quantitative real-time PCR (qRT-PCR), comparative ΔΔCt method, in fifteen patients with localized prostate cancer, treated with three-dimensional conformal radiotherapy (3DCRT). Nine subjects have experienced acute genitourinary (GU) radiotoxicity whereas six where without GU radiotoxicity. Results: Firstly, we detected the highest levels of miR-21 in ART group (p = 0.043) in the patients with acute GU radiotoxicity. Secondly, we found trend towards higher miR-21 levels and significantly higher levels of miR-146a/155 within the patients with acute GU toxicity than in patients without (p = 0.068, p = 0.016, and p = 0.010, respectively). Thirdly, we detected significant change in miR-146a/155 levels within the patients without acute GU radiotoxicity during RT p = 0.042, and p = 0.041, respectively). Conclusion: miR-21/146a/155 might be useful potential factors of radiosensitivity and acute genitourinary radiotoxicity in prostate cancer patients. miRNA might have great potential as predictors of various pathological conditions extracted from PBMCs. © 2018 Elsevier GmbH", journal = "Pathology - Research and Practice", title = "Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study", volume = "215", number = "4", pages = "626-631", doi = "10.1016/j.prp.2018.12.007" }
Kopčalić, K., Petrović, N., Stanojković, T. P., Stanković, V., Bukumirić, Z., Roganović, J., Mališić, E.,& Nikitović, M.. (2019). Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study. in Pathology - Research and Practice, 215(4), 626-631. https://doi.org/10.1016/j.prp.2018.12.007
Kopčalić K, Petrović N, Stanojković TP, Stanković V, Bukumirić Z, Roganović J, Mališić E, Nikitović M. Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study. in Pathology - Research and Practice. 2019;215(4):626-631. doi:10.1016/j.prp.2018.12.007 .
Kopčalić, Katarina, Petrović, Nina, Stanojković, Tatjana P., Stanković, Vesna, Bukumirić, Zoran, Roganović, Jelena, Mališić, Emina, Nikitović, Marina, "Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study" in Pathology - Research and Practice, 215, no. 4 (2019):626-631, https://doi.org/10.1016/j.prp.2018.12.007 . .