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Biological outcomes of γ-radiation induced DNA damages in breast and lung cancer cells pretreated with free radical scavengers

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Authors
Petković, Vladana
Keta, Otilija D.
Vidosavljević, Marija Z.
Incerti, Sebastien
Ristić-Fira, Aleksandra
Petrović, Ivan M.
Article (Published version)
,
© 2019 Taylor & Francis Group, LLC
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Abstract
Purpose: Investigation of effects on DNA of γ-irradiated human cancer cells pretreated with free radical scavengers is aimed to create reference data which would enable assessment of the relative efficiency of high linear energy transfer (LET) radiations used in hadron therapy, i.e. protons and carbon ions. Materials and methods: MCF-7 breast and HTB177 lung cancer cells are irradiated with γ-rays. To minimize indirect effects of irradiation, dimethyl sulfoxide (DMSO) or glycerol are applied as free radical scavengers. Biological response to irradiation is evaluated through clonogenic cell survival, immunocytochemical and cell cycle analysis, as well as expression of proteins involved in DNA damage response. Results: Examined cell lines reveal similar level of radioresistance. Application of scavengers leads to the rise of cell survival and decreases the number of DNA double strand breaks in irradiated cells. Differences in cell cycle and protein expression between the two cell lines a...re probably caused by different DNA damage repair mechanisms that are activated. Conclusion: The obtained results show that DMSO and glycerol have good scavenging capacity, and may be used to minimize DNA damage induced by free radicals. Therefore, they will be used as the reference for comparison with high LET irradiations, as well as good experimental data suitable for validation of numerical simulations. © 2019, © 2019 Taylor & Francis Group, LLC.

Keywords:
Breast cancer cells / non-small cell lung cancer cells / gamma-rays / free radical scavengers
Source:
International Journal of Radiation Biology, 2019, 95, 3, 274-285
Projects:
  • Radiosensitivity of human genome (RS-173046)
  • High Energy Physics with the CMS Detector (RS-171019)

DOI: 10.1080/09553002.2019.1549753

ISSN: 0955-3002

PubMed: 30451568

WoS: 000463581500003

Scopus: 2-s2.0-85060603904
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URI
http://vinar.vin.bg.ac.rs/handle/123456789/8414
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Vinča

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