Animal Models for Chronic Stress-Induced Oxidative Stress in the Spleen: The Role of Exercise and Catecholaminergic System
2018
Authors
Gavrilović, LjubicaStojiljković, Vesna
Popović, Nataša M.
Pejić, Snežana
Todorović, Ana
Pavlović, Ivan
Pajović, Snežana B.
Contributors
Bartholomew, IbehBook part (Published version)
Metadata
Show full item recordAbstract
We examined the effects of daily exercise on the gene expression of catecholamine biosynthetic enzymes (tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH), and phenyl
ethanolamine N-methyltransferase (PNMT)), vesicular monoamine transporter 2 (VMAT 2),
antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx)), concentrations of catecholamines (noradrenaline (NA) and adrenaline (A))
and malondialdehyde (MDA), activities of monoamine oxidase (MAO), and antioxidant
enzymes in the spleen of chronically psychosocially stressed rats. Exposure of chronically
stressed rats to exercise increased the levels of PNMT protein by 19%, VMAT 2 mRNA by
100%, NA by 160%, and A by 140%; decreased/unchanged MAO enzyme activity; returned
concentrations of MDA to control level; and increased CAT and GPx mRNA levels (50%
and 150%, respectively). Exercise induced the accumulation of the catecholamines and a
decrease of stress-induced oxidative stress in t...he spleen, which may significantly affect the
immune-neuroendocrine interactions in stress conditions. Also, exercise induced the catecholaminergic system and antioxidant defense to become more ready to a novel stressor,
which indicates that exercise may induce potentially positive physiological adaptations.
Our combined model of chronic social isolation and long-term daily treadmill running in
rats may be a good animal model in the research of therapeutic role of exercise in human
disease caused by chronic stress.
Keywords:
treadmill running / chronic social isolation / catecholamine / antioxidant enzymes / spleen / ratsSource:
Experimental Animal Models of Human Diseases : An Effective Therapeutic Strategy, (ed.) Bartholomew Ibeh, 2018, Ch. 14, 283-310Publisher:
- IntechOpen
- InTech
Funding / projects:
- Cellular and molecular basis of malignant and cardiovascular diseases-clinical implications (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41027)
- Acute coronary syndrome: investigation of vulnerability (plaque, blood and myocardium), optimal treatment and determination of the factors for the prognosis (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41022)
Note:
- Chapter of Experimental Animal Models of Human Diseases : An Effective Therapeutic Strategy / Edited by Bartholomew Ibeh. http://dx.doi.org/10.5772/66030
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Institution/Community
VinčaTY - CHAP AU - Gavrilović, Ljubica AU - Stojiljković, Vesna AU - Popović, Nataša M. AU - Pejić, Snežana AU - Todorović, Ana AU - Pavlović, Ivan AU - Pajović, Snežana B. PY - 2018 UR - https://vinar.vin.bg.ac.rs/handle/123456789/8406 AB - We examined the effects of daily exercise on the gene expression of catecholamine biosynthetic enzymes (tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH), and phenyl ethanolamine N-methyltransferase (PNMT)), vesicular monoamine transporter 2 (VMAT 2), antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx)), concentrations of catecholamines (noradrenaline (NA) and adrenaline (A)) and malondialdehyde (MDA), activities of monoamine oxidase (MAO), and antioxidant enzymes in the spleen of chronically psychosocially stressed rats. Exposure of chronically stressed rats to exercise increased the levels of PNMT protein by 19%, VMAT 2 mRNA by 100%, NA by 160%, and A by 140%; decreased/unchanged MAO enzyme activity; returned concentrations of MDA to control level; and increased CAT and GPx mRNA levels (50% and 150%, respectively). Exercise induced the accumulation of the catecholamines and a decrease of stress-induced oxidative stress in the spleen, which may significantly affect the immune-neuroendocrine interactions in stress conditions. Also, exercise induced the catecholaminergic system and antioxidant defense to become more ready to a novel stressor, which indicates that exercise may induce potentially positive physiological adaptations. Our combined model of chronic social isolation and long-term daily treadmill running in rats may be a good animal model in the research of therapeutic role of exercise in human disease caused by chronic stress. PB - IntechOpen PB - InTech T2 - Experimental Animal Models of Human Diseases : An Effective Therapeutic Strategy, (ed.) Bartholomew Ibeh T1 - Animal Models for Chronic Stress-Induced Oxidative Stress in the Spleen: The Role of Exercise and Catecholaminergic System IS - Ch. 14 SP - 283 EP - 310 DO - 10.5772/intechopen.70008 ER -
@inbook{ editor = "Bartholomew, Ibeh", author = "Gavrilović, Ljubica and Stojiljković, Vesna and Popović, Nataša M. and Pejić, Snežana and Todorović, Ana and Pavlović, Ivan and Pajović, Snežana B.", year = "2018", abstract = "We examined the effects of daily exercise on the gene expression of catecholamine biosynthetic enzymes (tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH), and phenyl ethanolamine N-methyltransferase (PNMT)), vesicular monoamine transporter 2 (VMAT 2), antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx)), concentrations of catecholamines (noradrenaline (NA) and adrenaline (A)) and malondialdehyde (MDA), activities of monoamine oxidase (MAO), and antioxidant enzymes in the spleen of chronically psychosocially stressed rats. Exposure of chronically stressed rats to exercise increased the levels of PNMT protein by 19%, VMAT 2 mRNA by 100%, NA by 160%, and A by 140%; decreased/unchanged MAO enzyme activity; returned concentrations of MDA to control level; and increased CAT and GPx mRNA levels (50% and 150%, respectively). Exercise induced the accumulation of the catecholamines and a decrease of stress-induced oxidative stress in the spleen, which may significantly affect the immune-neuroendocrine interactions in stress conditions. Also, exercise induced the catecholaminergic system and antioxidant defense to become more ready to a novel stressor, which indicates that exercise may induce potentially positive physiological adaptations. Our combined model of chronic social isolation and long-term daily treadmill running in rats may be a good animal model in the research of therapeutic role of exercise in human disease caused by chronic stress.", publisher = "IntechOpen, InTech", journal = "Experimental Animal Models of Human Diseases : An Effective Therapeutic Strategy, (ed.) Bartholomew Ibeh", booktitle = "Animal Models for Chronic Stress-Induced Oxidative Stress in the Spleen: The Role of Exercise and Catecholaminergic System", number = "Ch. 14", pages = "283-310", doi = "10.5772/intechopen.70008" }
Bartholomew, I., Gavrilović, L., Stojiljković, V., Popović, N. M., Pejić, S., Todorović, A., Pavlović, I.,& Pajović, S. B.. (2018). Animal Models for Chronic Stress-Induced Oxidative Stress in the Spleen: The Role of Exercise and Catecholaminergic System. in Experimental Animal Models of Human Diseases : An Effective Therapeutic Strategy, (ed.) Bartholomew Ibeh IntechOpen.(Ch. 14), 283-310. https://doi.org/10.5772/intechopen.70008
Bartholomew I, Gavrilović L, Stojiljković V, Popović NM, Pejić S, Todorović A, Pavlović I, Pajović SB. Animal Models for Chronic Stress-Induced Oxidative Stress in the Spleen: The Role of Exercise and Catecholaminergic System. in Experimental Animal Models of Human Diseases : An Effective Therapeutic Strategy, (ed.) Bartholomew Ibeh. 2018;(Ch. 14):283-310. doi:10.5772/intechopen.70008 .
Bartholomew, Ibeh, Gavrilović, Ljubica, Stojiljković, Vesna, Popović, Nataša M., Pejić, Snežana, Todorović, Ana, Pavlović, Ivan, Pajović, Snežana B., "Animal Models for Chronic Stress-Induced Oxidative Stress in the Spleen: The Role of Exercise and Catecholaminergic System" in Experimental Animal Models of Human Diseases : An Effective Therapeutic Strategy, (ed.) Bartholomew Ibeh, no. Ch. 14 (2018):283-310, https://doi.org/10.5772/intechopen.70008 . .