Expression of Antioxidant Enzymes in Patients with Uterine Polyp, Myoma, Hyperplasia, and Adenocarcinoma
Аутори
Todorović, AnaPejić, Snežana
Gavrilović, Ljubica
Pavlović, Ivan
Stojiljković, Vesna
Popović, Nataša M.
Pajović, Snežana B.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
We previously found that compared to patients with benign uterine diseases (polyps, myomas), patients with premalignant (hyperplasia simplex and complex) and malignant (adenocarcinoma) lesions had enhanced lipid peroxidation and altered uterine antioxidant enzyme (AOE) activities. To further elucidate the mechanism of the observed changes, we examined protein and mRNA levels of copper-zinc superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and transcription factor Nrf2. We also examined correlations of AOE expression with AOE activity, lipid hydroperoxides (LOOH) level, and level of Nrf2. Our results showed decreased CuZnSOD, CAT, and Nrf2 levels, and increased GPx and GR levels in hyperplasias, while in patients with adenocarcinoma, the level of CAT was decreased and GR was increased, compared to benign groups. Similar changes in mRNA levels were also detected, indicating predominantly translational control of the AOE expression. ...The positive correlation of enzyme expression/activity was recorded for CuZnSOD, GPx, and GR, but only among groups with benign diseases. Only GR and GPx expressions were positively correlated with LOOH. Nrf2 protein was positively correlated with mRNA levels of CuZnSOD and GR. Observed results indicate involvement of diverse redox mechanisms in etiopathogenesis of different gynecological diseases, and may improve redox-based approaches in current clinical practice.
Кључне речи:
antioxidant enzymes / polyps / myoma / hyperplasia / adenocarcinomapolyps / myoma / hyperplasia / adenocarcinomaИзвор:
Antioxidants, 2019, 8, 4, 97-Финансирање / пројекти:
- Ћелијске и молекулске основе малигних и кардиоваскуларних обољења-клиничке импликације (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41027)
- Молекуларно физиолошки биомониторинг аеробних организама заснован на одређивању биохемијских биомаркера оксидационог стреса (RS-MESTD-Basic Research (BR or ON)-173041)
DOI: 10.3390/antiox8040097
ISSN: 2076-3921
PubMed: 30978928
WoS: 000467294900021
Scopus: 2-s2.0-85070211697
Колекције
Институција/група
VinčaTY - JOUR AU - Todorović, Ana AU - Pejić, Snežana AU - Gavrilović, Ljubica AU - Pavlović, Ivan AU - Stojiljković, Vesna AU - Popović, Nataša M. AU - Pajović, Snežana B. PY - 2019 UR - https://www.mdpi.com/2076-3921/8/4/97 UR - https://vinar.vin.bg.ac.rs/handle/123456789/8403 AB - We previously found that compared to patients with benign uterine diseases (polyps, myomas), patients with premalignant (hyperplasia simplex and complex) and malignant (adenocarcinoma) lesions had enhanced lipid peroxidation and altered uterine antioxidant enzyme (AOE) activities. To further elucidate the mechanism of the observed changes, we examined protein and mRNA levels of copper-zinc superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and transcription factor Nrf2. We also examined correlations of AOE expression with AOE activity, lipid hydroperoxides (LOOH) level, and level of Nrf2. Our results showed decreased CuZnSOD, CAT, and Nrf2 levels, and increased GPx and GR levels in hyperplasias, while in patients with adenocarcinoma, the level of CAT was decreased and GR was increased, compared to benign groups. Similar changes in mRNA levels were also detected, indicating predominantly translational control of the AOE expression. The positive correlation of enzyme expression/activity was recorded for CuZnSOD, GPx, and GR, but only among groups with benign diseases. Only GR and GPx expressions were positively correlated with LOOH. Nrf2 protein was positively correlated with mRNA levels of CuZnSOD and GR. Observed results indicate involvement of diverse redox mechanisms in etiopathogenesis of different gynecological diseases, and may improve redox-based approaches in current clinical practice. T2 - Antioxidants T1 - Expression of Antioxidant Enzymes in Patients with Uterine Polyp, Myoma, Hyperplasia, and Adenocarcinoma VL - 8 IS - 4 SP - 97 DO - 10.3390/antiox8040097 ER -
@article{ author = "Todorović, Ana and Pejić, Snežana and Gavrilović, Ljubica and Pavlović, Ivan and Stojiljković, Vesna and Popović, Nataša M. and Pajović, Snežana B.", year = "2019", abstract = "We previously found that compared to patients with benign uterine diseases (polyps, myomas), patients with premalignant (hyperplasia simplex and complex) and malignant (adenocarcinoma) lesions had enhanced lipid peroxidation and altered uterine antioxidant enzyme (AOE) activities. To further elucidate the mechanism of the observed changes, we examined protein and mRNA levels of copper-zinc superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and transcription factor Nrf2. We also examined correlations of AOE expression with AOE activity, lipid hydroperoxides (LOOH) level, and level of Nrf2. Our results showed decreased CuZnSOD, CAT, and Nrf2 levels, and increased GPx and GR levels in hyperplasias, while in patients with adenocarcinoma, the level of CAT was decreased and GR was increased, compared to benign groups. Similar changes in mRNA levels were also detected, indicating predominantly translational control of the AOE expression. The positive correlation of enzyme expression/activity was recorded for CuZnSOD, GPx, and GR, but only among groups with benign diseases. Only GR and GPx expressions were positively correlated with LOOH. Nrf2 protein was positively correlated with mRNA levels of CuZnSOD and GR. Observed results indicate involvement of diverse redox mechanisms in etiopathogenesis of different gynecological diseases, and may improve redox-based approaches in current clinical practice.", journal = "Antioxidants", title = "Expression of Antioxidant Enzymes in Patients with Uterine Polyp, Myoma, Hyperplasia, and Adenocarcinoma", volume = "8", number = "4", pages = "97", doi = "10.3390/antiox8040097" }
Todorović, A., Pejić, S., Gavrilović, L., Pavlović, I., Stojiljković, V., Popović, N. M.,& Pajović, S. B.. (2019). Expression of Antioxidant Enzymes in Patients with Uterine Polyp, Myoma, Hyperplasia, and Adenocarcinoma. in Antioxidants, 8(4), 97. https://doi.org/10.3390/antiox8040097
Todorović A, Pejić S, Gavrilović L, Pavlović I, Stojiljković V, Popović NM, Pajović SB. Expression of Antioxidant Enzymes in Patients with Uterine Polyp, Myoma, Hyperplasia, and Adenocarcinoma. in Antioxidants. 2019;8(4):97. doi:10.3390/antiox8040097 .
Todorović, Ana, Pejić, Snežana, Gavrilović, Ljubica, Pavlović, Ivan, Stojiljković, Vesna, Popović, Nataša M., Pajović, Snežana B., "Expression of Antioxidant Enzymes in Patients with Uterine Polyp, Myoma, Hyperplasia, and Adenocarcinoma" in Antioxidants, 8, no. 4 (2019):97, https://doi.org/10.3390/antiox8040097 . .