Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression
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© 2019, Published by NRC Research Press
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This study investigated the effects of melatonin treatment on adrenal catecholamine content, synthesis, uptake, and vesicular transport induced by the chronic unpredictable mild stress (CUMS) model of depression in rats. This entailed quantifying the norepinephrine, epinephrine, mRNA, and protein levels of tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH), phenylethanolamine N-methyltransferase (PNMT), norepinephrine transporter (NET), and vesicular monoamine transporter 2 (VMAT2) in the adrenal medulla. CUMS caused a significant depletion of norepinephrine stores and protein levels of TH, DBH, and NET, whereas the gene expression of PNMT was increased. It was observed that melatonin treatment in the CUMS rats prevented the stress-induced decrease in norepinephrine content and the protein expression of TH, DBH, and NET in the adrenal medulla of chronically stressed rats. The present study demonstrates the stimulatory effect of melatonin on adrenomedullary synthesis, the uptake an...d content of catecholamine in the rat model of chronic stress-induced depression. © 2019, Published by NRC Research Press.
Keywords:
depression / melatonin / adrenal medulla / catecholamines / enzymes / transportersSource:
Canadian Journal of Physiology and Pharmacology, 2019, 97, 7, 685-690Funding / projects:
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- Peer-review version of the article available at: http://vinar.vin.bg.ac.rs/handle/123456789/8410
DOI: 10.1139/cjpp-2018-0612
ISSN: 0008-4212
PubMed: 30773040
WoS: 000472624400011
Scopus: 2-s2.0-85067946719
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VinčaTY - JOUR AU - Stefanović, Bojana AU - Spasojević, Nataša AU - Jovanović, Predrag AU - Dronjak, Slađana PY - 2019 UR - https://vinar.vin.bg.ac.rs/handle/123456789/8361 AB - This study investigated the effects of melatonin treatment on adrenal catecholamine content, synthesis, uptake, and vesicular transport induced by the chronic unpredictable mild stress (CUMS) model of depression in rats. This entailed quantifying the norepinephrine, epinephrine, mRNA, and protein levels of tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH), phenylethanolamine N-methyltransferase (PNMT), norepinephrine transporter (NET), and vesicular monoamine transporter 2 (VMAT2) in the adrenal medulla. CUMS caused a significant depletion of norepinephrine stores and protein levels of TH, DBH, and NET, whereas the gene expression of PNMT was increased. It was observed that melatonin treatment in the CUMS rats prevented the stress-induced decrease in norepinephrine content and the protein expression of TH, DBH, and NET in the adrenal medulla of chronically stressed rats. The present study demonstrates the stimulatory effect of melatonin on adrenomedullary synthesis, the uptake and content of catecholamine in the rat model of chronic stress-induced depression. © 2019, Published by NRC Research Press. T2 - Canadian Journal of Physiology and Pharmacology T1 - Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression VL - 97 IS - 7 SP - 685 EP - 690 DO - 10.1139/cjpp-2018-0612 ER -
@article{ author = "Stefanović, Bojana and Spasojević, Nataša and Jovanović, Predrag and Dronjak, Slađana", year = "2019", abstract = "This study investigated the effects of melatonin treatment on adrenal catecholamine content, synthesis, uptake, and vesicular transport induced by the chronic unpredictable mild stress (CUMS) model of depression in rats. This entailed quantifying the norepinephrine, epinephrine, mRNA, and protein levels of tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH), phenylethanolamine N-methyltransferase (PNMT), norepinephrine transporter (NET), and vesicular monoamine transporter 2 (VMAT2) in the adrenal medulla. CUMS caused a significant depletion of norepinephrine stores and protein levels of TH, DBH, and NET, whereas the gene expression of PNMT was increased. It was observed that melatonin treatment in the CUMS rats prevented the stress-induced decrease in norepinephrine content and the protein expression of TH, DBH, and NET in the adrenal medulla of chronically stressed rats. The present study demonstrates the stimulatory effect of melatonin on adrenomedullary synthesis, the uptake and content of catecholamine in the rat model of chronic stress-induced depression. © 2019, Published by NRC Research Press.", journal = "Canadian Journal of Physiology and Pharmacology", title = "Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression", volume = "97", number = "7", pages = "685-690", doi = "10.1139/cjpp-2018-0612" }
Stefanović, B., Spasojević, N., Jovanović, P.,& Dronjak, S.. (2019). Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression. in Canadian Journal of Physiology and Pharmacology, 97(7), 685-690. https://doi.org/10.1139/cjpp-2018-0612
Stefanović B, Spasojević N, Jovanović P, Dronjak S. Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression. in Canadian Journal of Physiology and Pharmacology. 2019;97(7):685-690. doi:10.1139/cjpp-2018-0612 .
Stefanović, Bojana, Spasojević, Nataša, Jovanović, Predrag, Dronjak, Slađana, "Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression" in Canadian Journal of Physiology and Pharmacology, 97, no. 7 (2019):685-690, https://doi.org/10.1139/cjpp-2018-0612 . .