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Effects of Intracerebroventricularly (ICV) Injected Ghrelin on Cardiac Inducible Nitric Oxide Synthase Activity/Expression in Obese Rats

Authorized Users Only
2015
Authors
Sudar, Emina
Jovanović, Aleksandra
Misirkić-Marjanović, Maja
Vučićević, Ljubica
Janjetović, Kristina D.
Isenović, Esma R.
Article (Published version)
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Abstract
The aim of this study was to examine the effects of ghrelin on regulation of cardiac inducible nitric oxide synthase (iNOS) activity/expression in high fat (HF), obese rats. For this study, male Wistar rats fed with HF diet (30 % fat) for 4 weeks were injected every 24 h for 5 days intracerebroventriculary (ICV) with ghrelin (0.3 nmol/5 mu l) or with an equal volume of phosphate buffered saline (PBS). Control rats were ICV injected with an equal volume of PBS. Glucose, insulin and nitric oxide (NO) concentrations were measured in serum, while arginase activity and citrulline concentrations were measured in heart lysate. Protein iNOS and regulatory subunit of nuclear factor-kappa B (NF kappa B-p65), phosphorylation of enzymes protein kinase B (Akt) at Ser(473), and extracellular signal-regulated kinases 1/2 (ERK1/2) at Tyr(202)/Tyr(204) were determined in heart lysate by Western blot. For gene expression of iNOS qRT-PCR was used. Results show significantly (p LT 0.01) higher serum NO pr...oduction in ghrelin treated HF rats compared with HF rats. Ghrelin significantly reduced citrulline concentration (p LT 0.05) and arginase activity (p LT 0.01) in HF rats. In ghrelin treated HF rats, gene and protein expression of iNOS and NF kappa B-p65 levels were significantly (p LT 0.05) increased compared with HF rats. Increased phosphorylation of Akt (p LT 0.01) and decreased (p LT 0.05) ERK1/2 phosphorylation were detected in HF ghrelin treated rats compared with HF rats hearts. Results from this study indicate that exogenous ghrelin induces expression and activity of cardiac iNOS via Akt phosphorylation followed by NF kappa B activation in HF rats.

Keywords:
ghrelin / heart / nitric oxide / nitric oxide synthase / protein kinase B / extracellular signal-regulated kinases 1/2
Source:
Experimental and Clinical Endocrinology and Diabetes, 2015, 123, 10, 581-588
Publisher:
  • Georg Thieme Verlag KG
Funding / projects:
  • Hormonal regulation of expression and activity of the nitric oxide synthase and sodium-potassium pump in experimental models of insulin resistance, diabetes and cardiovascular disorders (RS-173033)
  • Modulation of intracellular energy balance-controlling signalling pathways in therapy of cancer and neuro-immuno-endocrine disorders (RS-41025)

DOI: 10.1055/s-0035-1559758

ISSN: 0947-7349; 1439-3646

WoS: 000365626900002

Scopus: 2-s2.0-84948184682
[ Google Scholar ]
10
6
URI
https://vinar.vin.bg.ac.rs/handle/123456789/832
Collections
  • WoS Import
Institution/Community
Vinča
TY  - JOUR
AU  - Sudar, Emina
AU  - Jovanović, Aleksandra
AU  - Misirkić-Marjanović, Maja
AU  - Vučićević, Ljubica
AU  - Janjetović, Kristina D.
AU  - Isenović, Esma R.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/832
AB  - The aim of this study was to examine the effects of ghrelin on regulation of cardiac inducible nitric oxide synthase (iNOS) activity/expression in high fat (HF), obese rats. For this study, male Wistar rats fed with HF diet (30 % fat) for 4 weeks were injected every 24 h for 5 days intracerebroventriculary (ICV) with ghrelin (0.3 nmol/5 mu l) or with an equal volume of phosphate buffered saline (PBS). Control rats were ICV injected with an equal volume of PBS. Glucose, insulin and nitric oxide (NO) concentrations were measured in serum, while arginase activity and citrulline concentrations were measured in heart lysate. Protein iNOS and regulatory subunit of nuclear factor-kappa B (NF kappa B-p65), phosphorylation of enzymes protein kinase B (Akt) at Ser(473), and extracellular signal-regulated kinases 1/2 (ERK1/2) at Tyr(202)/Tyr(204) were determined in heart lysate by Western blot. For gene expression of iNOS qRT-PCR was used. Results show significantly (p LT 0.01) higher serum NO production in ghrelin treated HF rats compared with HF rats. Ghrelin significantly reduced citrulline concentration (p LT 0.05) and arginase activity (p LT 0.01) in HF rats. In ghrelin treated HF rats, gene and protein expression of iNOS and NF kappa B-p65 levels were significantly (p LT 0.05) increased compared with HF rats. Increased phosphorylation of Akt (p LT 0.01) and decreased (p LT 0.05) ERK1/2 phosphorylation were detected in HF ghrelin treated rats compared with HF rats hearts. Results from this study indicate that exogenous ghrelin induces expression and activity of cardiac iNOS via Akt phosphorylation followed by NF kappa B activation in HF rats.
PB  - Georg Thieme Verlag KG
T2  - Experimental and Clinical Endocrinology and Diabetes
T1  - Effects of Intracerebroventricularly (ICV) Injected Ghrelin on Cardiac Inducible Nitric Oxide Synthase Activity/Expression in Obese Rats
VL  - 123
IS  - 10
SP  - 581
EP  - 588
DO  - 10.1055/s-0035-1559758
ER  - 
@article{
author = "Sudar, Emina and Jovanović, Aleksandra and Misirkić-Marjanović, Maja and Vučićević, Ljubica and Janjetović, Kristina D. and Isenović, Esma R.",
year = "2015",
abstract = "The aim of this study was to examine the effects of ghrelin on regulation of cardiac inducible nitric oxide synthase (iNOS) activity/expression in high fat (HF), obese rats. For this study, male Wistar rats fed with HF diet (30 % fat) for 4 weeks were injected every 24 h for 5 days intracerebroventriculary (ICV) with ghrelin (0.3 nmol/5 mu l) or with an equal volume of phosphate buffered saline (PBS). Control rats were ICV injected with an equal volume of PBS. Glucose, insulin and nitric oxide (NO) concentrations were measured in serum, while arginase activity and citrulline concentrations were measured in heart lysate. Protein iNOS and regulatory subunit of nuclear factor-kappa B (NF kappa B-p65), phosphorylation of enzymes protein kinase B (Akt) at Ser(473), and extracellular signal-regulated kinases 1/2 (ERK1/2) at Tyr(202)/Tyr(204) were determined in heart lysate by Western blot. For gene expression of iNOS qRT-PCR was used. Results show significantly (p LT 0.01) higher serum NO production in ghrelin treated HF rats compared with HF rats. Ghrelin significantly reduced citrulline concentration (p LT 0.05) and arginase activity (p LT 0.01) in HF rats. In ghrelin treated HF rats, gene and protein expression of iNOS and NF kappa B-p65 levels were significantly (p LT 0.05) increased compared with HF rats. Increased phosphorylation of Akt (p LT 0.01) and decreased (p LT 0.05) ERK1/2 phosphorylation were detected in HF ghrelin treated rats compared with HF rats hearts. Results from this study indicate that exogenous ghrelin induces expression and activity of cardiac iNOS via Akt phosphorylation followed by NF kappa B activation in HF rats.",
publisher = "Georg Thieme Verlag KG",
journal = "Experimental and Clinical Endocrinology and Diabetes",
title = "Effects of Intracerebroventricularly (ICV) Injected Ghrelin on Cardiac Inducible Nitric Oxide Synthase Activity/Expression in Obese Rats",
volume = "123",
number = "10",
pages = "581-588",
doi = "10.1055/s-0035-1559758"
}
Sudar, E., Jovanović, A., Misirkić-Marjanović, M., Vučićević, L., Janjetović, K. D.,& Isenović, E. R.. (2015). Effects of Intracerebroventricularly (ICV) Injected Ghrelin on Cardiac Inducible Nitric Oxide Synthase Activity/Expression in Obese Rats. in Experimental and Clinical Endocrinology and Diabetes
Georg Thieme Verlag KG., 123(10), 581-588.
https://doi.org/10.1055/s-0035-1559758
Sudar E, Jovanović A, Misirkić-Marjanović M, Vučićević L, Janjetović KD, Isenović ER. Effects of Intracerebroventricularly (ICV) Injected Ghrelin on Cardiac Inducible Nitric Oxide Synthase Activity/Expression in Obese Rats. in Experimental and Clinical Endocrinology and Diabetes. 2015;123(10):581-588.
doi:10.1055/s-0035-1559758 .
Sudar, Emina, Jovanović, Aleksandra, Misirkić-Marjanović, Maja, Vučićević, Ljubica, Janjetović, Kristina D., Isenović, Esma R., "Effects of Intracerebroventricularly (ICV) Injected Ghrelin on Cardiac Inducible Nitric Oxide Synthase Activity/Expression in Obese Rats" in Experimental and Clinical Endocrinology and Diabetes, 123, no. 10 (2015):581-588,
https://doi.org/10.1055/s-0035-1559758 . .

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