VinaR - Repository of the Vinča Nuclear Institute
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   Vinar
  • Vinča
  • Radovi istraživača
  • View Item
  •   Vinar
  • Vinča
  • Radovi istraživača
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Spatial Distribution and Expression of Ectonucleotidases in Rat Hippocampus After Removal of Ovaries and Estradiol Replacement

Authorized Users Only
2019
Authors
Grković, Ivana
Mitrović, Nataša Lj.
Dragić, Milorad
Adžić, Marija
Drakulić, Dunja R.
Nedeljković, Nadežda
article (publishedVersion)
,
© 2018, Springer Science+Business Media, LLC, part of Springer Nature
Metadata
Show full item record
Abstract
Purinergic signaling is the main synaptic and non-synaptic signaling system in brain. ATP acts as a fast excitatory transmitter, while adenosine sets a global inhibitory tone within hippocampal neuronal networks. ATP and adenosine are interconnected by ectonucleotidase enzymes, which convert ATP to adenosine. Existing data point to the converging roles of ovarian steroids and purinergic signaling in synapse formation and refinement and synapse activity in the hippocampus. Therefore, in the present study, we have used enzyme histochemistry and expression analysis to obtain data on spatial distribution and expression of ecto-enzymes NTPDase1, NTPDase2, and ecto-5-nucleotidase (eN) after removal of ovaries (OVX) and estradiol replacement (E2) in female rat hippocampus. The results show that target ectonucleotidases are predominantly localized in synapse-rich hippocampal layers. The most represented NTPDase in the hippocampal tissue is NTPDase2, being at the same time the mostly affected e...ctonucleotidase by OVX and E2. Specifically, OVX decreases the expression of NTPDase2 and eN, whereas E2 restores their expression to control level. Impact of OVX and E2 on ectonucleotidase expression was also examined in purified synaptosome (SYN) and gliosome (GLIO) fractions. Data reveal that SYN expresses NTPDase1 and NTPDase2, both of which are reduced following OVX and restored with E2. GLIO exhibits NTPDase2-mediated ATP hydrolysis, which falls in OVX, and recovers by E2. These changes in the activity occur without parallel changes in NTPDase2-protein abundance. The same holds for eN. The lack of correlation between NTPDase2 and eN activities and their respective protein abundances suggest a non-genomic mode of E2 action, which is studied further in primary astrocyte culture. Since ovarian steroids shape hippocampal synaptic networks and regulate ectonucleotidase activities, it is possible that cognitive deficits seen after ovary removal may arise from the loss of E2 modulatory actions on ectonucleotidase expression in the hippocampus.

Keywords:
Ectonucleotidases / Hippocampus / Ovariectomy / Estradiol / Astrocytes
Source:
Molecular Neurobiology, 2019, 56, 3, 1933-1945
Funding / projects:
  • info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173044/RS// (RS-173044)
  • info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41014/RS// (RS-41014)

DOI: 10.1007/s12035-018-1217-3

ISSN: 0893-7648; 1559-1182

PubMed: 29978426

WoS: 000460163700030

Scopus: 2-s2.0-85049558913
[ Google Scholar ]
URI
http://link.springer.com/10.1007/s12035-018-1217-3
https://vinar.vin.bg.ac.rs/handle/123456789/8102
Collections
  • Radovi istraživača
Institution/Community
Vinča

DSpace software copyright © 2002-2015  DuraSpace
About the VinaR Repository | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceInstitutions/communitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About the VinaR Repository | Send Feedback

OpenAIRERCUB