Effects of 17 beta-estradiol on cardiac Na+/K+-ATPase in high fat diet fed rats
Само за регистроване кориснике
2015
Аутори
Obradović, Milan M.Zafirović, Sonja
Jovanović, Aleksandra
Sudar, Emina
Mousa, Shaker A.
Labudović-Borović, Milica
Isenović, Esma R.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of this study was to investigate in vivo effects of estradiol on Na+/K+-ATPase activity/expression in high fat (HF) diet fed rats. Adult male Wistar rats were fed normally (Control, n = 7) or with a HF diet (Obese, n = 14) for 10 weeks. After 10 weeks, half of the obese rats were treated with estradiol (Obese + Estradiol, n = 7, 40 mu g/kg, i.p.) as a bolus injection and 24 h after treatment all the rats were sacrificed. Estradiol in vivo in obese rats in comparison with obese non-treated rats led to a statistically significant increase in concentration of serum Na+ (p LT 0.05), Na+/K+-ATPase activity (p LT 0.01), expression of alpha 1 (p LT 0.01) and alpha 2 (p LT 0.05) subunit of Na+/K+-ATPase, both PI3K subunits p85 (p LT 0.01), p110 (p LT 0.05), and association of IRS-1 with p85 (p LT 0.05), while significantly decrease expression of AT1 (p LT 0.05) and Rho A (p LT 0.01) proteins. Our results suggest that estradiol in vivo in pathophysiological conditions, such as obesity a...ccompanied with insulin resistance stimulates activity and expression of Na+/K+-ATPase by a mechanism that involves the participation of IRS-1/PI3K/Akt signaling. In addition, the decreasing level of AT1 and Rho A proteins estradiol probably attenuates the detrimental effect of obesity to decreased IRS-1/P13K association and consequently reduce Na+/K+-ATPase activity/expression. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
Кључне речи:
Estradiol / Sodium potassium adenosine-triphosphatase / Obesity / Insulin resistance / High fat dietИзвор:
Molecular and Cellular Endocrinology, 2015, 416, C, 46-56Издавач:
- Elsevier
Финансирање / пројекти:
- Хормонска регулација експресије и активности азот оксид синтазе и натријум-калијумове пумпе у експерименталним моделима инсулинске резистенције, дијабетеса и кардиоваскуларних поремећаја (RS-MESTD-Basic Research (BR or ON)-173033)
DOI: 10.1016/j.mce.2015.08.020
ISSN: 0303-7207
PubMed: 26284496
WoS: 000364267800006
Scopus: 2-s2.0-84943625339
Колекције
Институција/група
VinčaTY - JOUR AU - Obradović, Milan M. AU - Zafirović, Sonja AU - Jovanović, Aleksandra AU - Sudar, Emina AU - Mousa, Shaker A. AU - Labudović-Borović, Milica AU - Isenović, Esma R. PY - 2015 UR - https://vinar.vin.bg.ac.rs/handle/123456789/803 AB - The aim of this study was to investigate in vivo effects of estradiol on Na+/K+-ATPase activity/expression in high fat (HF) diet fed rats. Adult male Wistar rats were fed normally (Control, n = 7) or with a HF diet (Obese, n = 14) for 10 weeks. After 10 weeks, half of the obese rats were treated with estradiol (Obese + Estradiol, n = 7, 40 mu g/kg, i.p.) as a bolus injection and 24 h after treatment all the rats were sacrificed. Estradiol in vivo in obese rats in comparison with obese non-treated rats led to a statistically significant increase in concentration of serum Na+ (p LT 0.05), Na+/K+-ATPase activity (p LT 0.01), expression of alpha 1 (p LT 0.01) and alpha 2 (p LT 0.05) subunit of Na+/K+-ATPase, both PI3K subunits p85 (p LT 0.01), p110 (p LT 0.05), and association of IRS-1 with p85 (p LT 0.05), while significantly decrease expression of AT1 (p LT 0.05) and Rho A (p LT 0.01) proteins. Our results suggest that estradiol in vivo in pathophysiological conditions, such as obesity accompanied with insulin resistance stimulates activity and expression of Na+/K+-ATPase by a mechanism that involves the participation of IRS-1/PI3K/Akt signaling. In addition, the decreasing level of AT1 and Rho A proteins estradiol probably attenuates the detrimental effect of obesity to decreased IRS-1/P13K association and consequently reduce Na+/K+-ATPase activity/expression. (C) 2015 Elsevier Ireland Ltd. All rights reserved. PB - Elsevier T2 - Molecular and Cellular Endocrinology T1 - Effects of 17 beta-estradiol on cardiac Na+/K+-ATPase in high fat diet fed rats VL - 416 IS - C SP - 46 EP - 56 DO - 10.1016/j.mce.2015.08.020 ER -
@article{ author = "Obradović, Milan M. and Zafirović, Sonja and Jovanović, Aleksandra and Sudar, Emina and Mousa, Shaker A. and Labudović-Borović, Milica and Isenović, Esma R.", year = "2015", abstract = "The aim of this study was to investigate in vivo effects of estradiol on Na+/K+-ATPase activity/expression in high fat (HF) diet fed rats. Adult male Wistar rats were fed normally (Control, n = 7) or with a HF diet (Obese, n = 14) for 10 weeks. After 10 weeks, half of the obese rats were treated with estradiol (Obese + Estradiol, n = 7, 40 mu g/kg, i.p.) as a bolus injection and 24 h after treatment all the rats were sacrificed. Estradiol in vivo in obese rats in comparison with obese non-treated rats led to a statistically significant increase in concentration of serum Na+ (p LT 0.05), Na+/K+-ATPase activity (p LT 0.01), expression of alpha 1 (p LT 0.01) and alpha 2 (p LT 0.05) subunit of Na+/K+-ATPase, both PI3K subunits p85 (p LT 0.01), p110 (p LT 0.05), and association of IRS-1 with p85 (p LT 0.05), while significantly decrease expression of AT1 (p LT 0.05) and Rho A (p LT 0.01) proteins. Our results suggest that estradiol in vivo in pathophysiological conditions, such as obesity accompanied with insulin resistance stimulates activity and expression of Na+/K+-ATPase by a mechanism that involves the participation of IRS-1/PI3K/Akt signaling. In addition, the decreasing level of AT1 and Rho A proteins estradiol probably attenuates the detrimental effect of obesity to decreased IRS-1/P13K association and consequently reduce Na+/K+-ATPase activity/expression. (C) 2015 Elsevier Ireland Ltd. All rights reserved.", publisher = "Elsevier", journal = "Molecular and Cellular Endocrinology", title = "Effects of 17 beta-estradiol on cardiac Na+/K+-ATPase in high fat diet fed rats", volume = "416", number = "C", pages = "46-56", doi = "10.1016/j.mce.2015.08.020" }
Obradović, M. M., Zafirović, S., Jovanović, A., Sudar, E., Mousa, S. A., Labudović-Borović, M.,& Isenović, E. R.. (2015). Effects of 17 beta-estradiol on cardiac Na+/K+-ATPase in high fat diet fed rats. in Molecular and Cellular Endocrinology Elsevier., 416(C), 46-56. https://doi.org/10.1016/j.mce.2015.08.020
Obradović MM, Zafirović S, Jovanović A, Sudar E, Mousa SA, Labudović-Borović M, Isenović ER. Effects of 17 beta-estradiol on cardiac Na+/K+-ATPase in high fat diet fed rats. in Molecular and Cellular Endocrinology. 2015;416(C):46-56. doi:10.1016/j.mce.2015.08.020 .
Obradović, Milan M., Zafirović, Sonja, Jovanović, Aleksandra, Sudar, Emina, Mousa, Shaker A., Labudović-Borović, Milica, Isenović, Esma R., "Effects of 17 beta-estradiol on cardiac Na+/K+-ATPase in high fat diet fed rats" in Molecular and Cellular Endocrinology, 416, no. C (2015):46-56, https://doi.org/10.1016/j.mce.2015.08.020 . .