HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia
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2018
Authors
Životić, IvanĐurić, Tamara
Stanković, Aleksandra
Stanković, Goran
Milašinović, Dejan
Dekleva, Milica
Marković-Nikolić, Nataša
Alavantić, Dragan
Živković, Maja
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Show full item recordAbstract
Aim: Myocardial infarction (MI) incidence is still a major burden in the
terms of premature death and disability world-wide. Genetic studies up to
date explained a limited portion of disease inheritance. Recently, the DT
haplotype of variants rs36212560 I/D (insertion/deletion) and 2275888 T/C
in HACD gene (9p21.3) have been significantly associated with the risk of
carotid plaque occurrence among males.
Methods: We aimed to validate these results by investigating 518 MI patients (366 males) and 303 (167 males) healthy controls from Serbia. Also, he HACD4 gene expression analysis has been conducted in the peripheral
blood mononuclear cells of 72 MI patients, 6 months after the MI. Results
were obtained using ABI-Prism 7500 RT-PCR for rs2275888 allelic
discrimination and HACD4 mRNA relative quantitation. PCR and acrylamide gel electrophoresis were used to distinguish 5 base pairs
rs36212560 insertion deletion polymorphisms.
Results: Haplotype analysis (using Thesias software)... showed that DT
haplotype carriers had significantly higher risk for MI (OR ¼ 1.42, CI 1.08-
1.85, p¼0.01) compared with most frequent IT haplotype. In gender
separated groups association remained significant only among males (OR
¼ 1.76, 1.266-2.46, p¼0.0008). Results were adjusted for MI traditional risk
factors (Age, BMI, HDLC, LDLC, Tg, hypertension and smoking). Investigated
genetic variants were not associated with HACD4 expression. Significant
correlation was found between HACD4 mRNA level and age (r¼0.36,
p¼0.001).
Conclusions: We have shown that rs36212560 and rs2275888 DT haplotype from HACD4 gene is significantly and independently associated with
the MI occurrence in males. Additional studies are needed to confirm these
results.
Source:
Atherosclerosis, 2018, 275, e210-e211Note:
- 86th Congress of the European-Atherosclerosis-Society (EAS), Lisbon,PORTUGAL, MAY 05-08, 2018
DOI: 10.1016/j.atherosclerosis.2018.06.657
ISSN: 0021-9150; 1879-1484
WoS: 000442512600669
[ Google Scholar ]URI
https://linkinghub.elsevier.com/retrieve/pii/S0021915018309699https://vinar.vin.bg.ac.rs/handle/123456789/7953
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VinčaTY - CONF AU - Životić, Ivan AU - Đurić, Tamara AU - Stanković, Aleksandra AU - Stanković, Goran AU - Milašinović, Dejan AU - Dekleva, Milica AU - Marković-Nikolić, Nataša AU - Alavantić, Dragan AU - Živković, Maja PY - 2018 UR - https://linkinghub.elsevier.com/retrieve/pii/S0021915018309699 UR - https://vinar.vin.bg.ac.rs/handle/123456789/7953 AB - Aim: Myocardial infarction (MI) incidence is still a major burden in the terms of premature death and disability world-wide. Genetic studies up to date explained a limited portion of disease inheritance. Recently, the DT haplotype of variants rs36212560 I/D (insertion/deletion) and 2275888 T/C in HACD gene (9p21.3) have been significantly associated with the risk of carotid plaque occurrence among males. Methods: We aimed to validate these results by investigating 518 MI patients (366 males) and 303 (167 males) healthy controls from Serbia. Also, he HACD4 gene expression analysis has been conducted in the peripheral blood mononuclear cells of 72 MI patients, 6 months after the MI. Results were obtained using ABI-Prism 7500 RT-PCR for rs2275888 allelic discrimination and HACD4 mRNA relative quantitation. PCR and acrylamide gel electrophoresis were used to distinguish 5 base pairs rs36212560 insertion deletion polymorphisms. Results: Haplotype analysis (using Thesias software) showed that DT haplotype carriers had significantly higher risk for MI (OR ¼ 1.42, CI 1.08- 1.85, p¼0.01) compared with most frequent IT haplotype. In gender separated groups association remained significant only among males (OR ¼ 1.76, 1.266-2.46, p¼0.0008). Results were adjusted for MI traditional risk factors (Age, BMI, HDLC, LDLC, Tg, hypertension and smoking). Investigated genetic variants were not associated with HACD4 expression. Significant correlation was found between HACD4 mRNA level and age (r¼0.36, p¼0.001). Conclusions: We have shown that rs36212560 and rs2275888 DT haplotype from HACD4 gene is significantly and independently associated with the MI occurrence in males. Additional studies are needed to confirm these results. C3 - Atherosclerosis T1 - HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia VL - 275 SP - e210 EP - e211 DO - 10.1016/j.atherosclerosis.2018.06.657 ER -
@conference{ author = "Životić, Ivan and Đurić, Tamara and Stanković, Aleksandra and Stanković, Goran and Milašinović, Dejan and Dekleva, Milica and Marković-Nikolić, Nataša and Alavantić, Dragan and Živković, Maja", year = "2018", abstract = "Aim: Myocardial infarction (MI) incidence is still a major burden in the terms of premature death and disability world-wide. Genetic studies up to date explained a limited portion of disease inheritance. Recently, the DT haplotype of variants rs36212560 I/D (insertion/deletion) and 2275888 T/C in HACD gene (9p21.3) have been significantly associated with the risk of carotid plaque occurrence among males. Methods: We aimed to validate these results by investigating 518 MI patients (366 males) and 303 (167 males) healthy controls from Serbia. Also, he HACD4 gene expression analysis has been conducted in the peripheral blood mononuclear cells of 72 MI patients, 6 months after the MI. Results were obtained using ABI-Prism 7500 RT-PCR for rs2275888 allelic discrimination and HACD4 mRNA relative quantitation. PCR and acrylamide gel electrophoresis were used to distinguish 5 base pairs rs36212560 insertion deletion polymorphisms. Results: Haplotype analysis (using Thesias software) showed that DT haplotype carriers had significantly higher risk for MI (OR ¼ 1.42, CI 1.08- 1.85, p¼0.01) compared with most frequent IT haplotype. In gender separated groups association remained significant only among males (OR ¼ 1.76, 1.266-2.46, p¼0.0008). Results were adjusted for MI traditional risk factors (Age, BMI, HDLC, LDLC, Tg, hypertension and smoking). Investigated genetic variants were not associated with HACD4 expression. Significant correlation was found between HACD4 mRNA level and age (r¼0.36, p¼0.001). Conclusions: We have shown that rs36212560 and rs2275888 DT haplotype from HACD4 gene is significantly and independently associated with the MI occurrence in males. Additional studies are needed to confirm these results.", journal = "Atherosclerosis", title = "HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia", volume = "275", pages = "e210-e211", doi = "10.1016/j.atherosclerosis.2018.06.657" }
Životić, I., Đurić, T., Stanković, A., Stanković, G., Milašinović, D., Dekleva, M., Marković-Nikolić, N., Alavantić, D.,& Živković, M.. (2018). HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia. in Atherosclerosis, 275, e210-e211. https://doi.org/10.1016/j.atherosclerosis.2018.06.657
Životić I, Đurić T, Stanković A, Stanković G, Milašinović D, Dekleva M, Marković-Nikolić N, Alavantić D, Živković M. HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia. in Atherosclerosis. 2018;275:e210-e211. doi:10.1016/j.atherosclerosis.2018.06.657 .
Životić, Ivan, Đurić, Tamara, Stanković, Aleksandra, Stanković, Goran, Milašinović, Dejan, Dekleva, Milica, Marković-Nikolić, Nataša, Alavantić, Dragan, Živković, Maja, "HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia" in Atherosclerosis, 275 (2018):e210-e211, https://doi.org/10.1016/j.atherosclerosis.2018.06.657 . .