Wild Bilberry (Vaccinium myrtillus L., Ericaceae) from Montenegro as a Source of Antioxidants for Use in the Production of Nutraceuticals
Authors
Brašanac-Vukanović, SnežanaMutić, Jelena J.
Stanković, Dalibor M.
Arsić, Ivana A.
Blagojević, Nada Z.
Vukašinović-Pešić, Vesna L.
Tadić, Vanja M.
Article (Published version)
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The aim of this study was to establish correlation of chemical composition and antioxidant activity of bilberry plants from Montenegro. Total phenolic, tannin, flavonoid, procyanidin and anthocyanin contents were determined in fruits and leaves extracts using spectrophotometric methods, while the measurements of metal content was carried out in an Inductively Coupled Atomic Emission Spectrometer. Qualitative and quantitative analyses of major phenolics were achieved by HPLC. In the investigated extracts, the most abundant phenolic was chlorogenic acid, followed by protocatechuic acid, while resveratrol, isoquercetin, quecetin and hyperoside were also present in significant quantities. Antioxidant potential was evaluated using two in vitro assays—FRAP and DPPH—being in the accordance with the cyclic voltammetry tests, performed as well. The results revealed that all the investigated extracts were rich in phenolic and essential mineral constituents, with significant antioxidant activity,... depending on the polyphenolic and mineral contents, which was confirmed by principal component analysis.
Keywords:
Vaccinium myrtillus / phenolic compounds / metals / antioxidant activity / cyclic voltammetry / principal component analysisSource:
Molecules, 2018, 23, 8, 1864-Funding / projects:
- Functional physiologically active plant materials with additional values for application in pharmaceutical and food industry (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45017)
- Application of advanced oxidation processes and nanostructured oxide materials for the removal of pollutants from the environment, development and optimisation of instrumental techniques for efficiency monitoring (RS-MESTD-Basic Research (BR or ON)-172030)
- Ministry of Education, Science and Technological Development (Serbia) and Ministry of Science (Montenegro) : “Chemical, biochemical and microbiological characterization of Vaccinium myrtillus as potential source of valuable phytoconstituents in functional food and dermocosmoceutics (Vaccinium myrtyllus L., Ericaceae-old remedy, new application” (451-03-01414/2016-09/1)
DOI: 10.3390/molecules23081864
ISSN: 1420-3049
PubMed: 30050005
WoS: 000445295500029
Scopus: 2-s2.0-85052019835
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VinčaTY - JOUR AU - Brašanac-Vukanović, Snežana AU - Mutić, Jelena J. AU - Stanković, Dalibor M. AU - Arsić, Ivana A. AU - Blagojević, Nada Z. AU - Vukašinović-Pešić, Vesna L. AU - Tadić, Vanja M. PY - 2018 UR - http://www.mdpi.com/1420-3049/23/8/1864 UR - https://vinar.vin.bg.ac.rs/handle/123456789/7848 AB - The aim of this study was to establish correlation of chemical composition and antioxidant activity of bilberry plants from Montenegro. Total phenolic, tannin, flavonoid, procyanidin and anthocyanin contents were determined in fruits and leaves extracts using spectrophotometric methods, while the measurements of metal content was carried out in an Inductively Coupled Atomic Emission Spectrometer. Qualitative and quantitative analyses of major phenolics were achieved by HPLC. In the investigated extracts, the most abundant phenolic was chlorogenic acid, followed by protocatechuic acid, while resveratrol, isoquercetin, quecetin and hyperoside were also present in significant quantities. Antioxidant potential was evaluated using two in vitro assays—FRAP and DPPH—being in the accordance with the cyclic voltammetry tests, performed as well. The results revealed that all the investigated extracts were rich in phenolic and essential mineral constituents, with significant antioxidant activity, depending on the polyphenolic and mineral contents, which was confirmed by principal component analysis. T2 - Molecules T1 - Wild Bilberry (Vaccinium myrtillus L., Ericaceae) from Montenegro as a Source of Antioxidants for Use in the Production of Nutraceuticals VL - 23 IS - 8 SP - 1864 DO - 10.3390/molecules23081864 ER -
@article{ author = "Brašanac-Vukanović, Snežana and Mutić, Jelena J. and Stanković, Dalibor M. and Arsić, Ivana A. and Blagojević, Nada Z. and Vukašinović-Pešić, Vesna L. and Tadić, Vanja M.", year = "2018", abstract = "The aim of this study was to establish correlation of chemical composition and antioxidant activity of bilberry plants from Montenegro. Total phenolic, tannin, flavonoid, procyanidin and anthocyanin contents were determined in fruits and leaves extracts using spectrophotometric methods, while the measurements of metal content was carried out in an Inductively Coupled Atomic Emission Spectrometer. Qualitative and quantitative analyses of major phenolics were achieved by HPLC. In the investigated extracts, the most abundant phenolic was chlorogenic acid, followed by protocatechuic acid, while resveratrol, isoquercetin, quecetin and hyperoside were also present in significant quantities. Antioxidant potential was evaluated using two in vitro assays—FRAP and DPPH—being in the accordance with the cyclic voltammetry tests, performed as well. The results revealed that all the investigated extracts were rich in phenolic and essential mineral constituents, with significant antioxidant activity, depending on the polyphenolic and mineral contents, which was confirmed by principal component analysis.", journal = "Molecules", title = "Wild Bilberry (Vaccinium myrtillus L., Ericaceae) from Montenegro as a Source of Antioxidants for Use in the Production of Nutraceuticals", volume = "23", number = "8", pages = "1864", doi = "10.3390/molecules23081864" }
Brašanac-Vukanović, S., Mutić, J. J., Stanković, D. M., Arsić, I. A., Blagojević, N. Z., Vukašinović-Pešić, V. L.,& Tadić, V. M.. (2018). Wild Bilberry (Vaccinium myrtillus L., Ericaceae) from Montenegro as a Source of Antioxidants for Use in the Production of Nutraceuticals. in Molecules, 23(8), 1864. https://doi.org/10.3390/molecules23081864
Brašanac-Vukanović S, Mutić JJ, Stanković DM, Arsić IA, Blagojević NZ, Vukašinović-Pešić VL, Tadić VM. Wild Bilberry (Vaccinium myrtillus L., Ericaceae) from Montenegro as a Source of Antioxidants for Use in the Production of Nutraceuticals. in Molecules. 2018;23(8):1864. doi:10.3390/molecules23081864 .
Brašanac-Vukanović, Snežana, Mutić, Jelena J., Stanković, Dalibor M., Arsić, Ivana A., Blagojević, Nada Z., Vukašinović-Pešić, Vesna L., Tadić, Vanja M., "Wild Bilberry (Vaccinium myrtillus L., Ericaceae) from Montenegro as a Source of Antioxidants for Use in the Production of Nutraceuticals" in Molecules, 23, no. 8 (2018):1864, https://doi.org/10.3390/molecules23081864 . .