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dc.creatorMilivojević, Dušan
dc.creatorŠumonja, Neven
dc.creatorMedić, Strahinja
dc.creatorPavić, Aleksandar B.
dc.creatorMorić, Ivana
dc.creatorVasiljević, Branka
dc.creatorSenerović, Lidija
dc.creatorNikodinović-Runić, Jasmina
dc.date.accessioned2018-10-01T07:16:23Z
dc.date.available2018-10-01T07:16:23Z
dc.date.issued2018
dc.identifier.issn2049-632X
dc.identifier.urihttps://academic.oup.com/femspd/article/doi/10.1093/femspd/fty041/4978417
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/7822
dc.description.abstractPseudomonas aeruginosa has been amongst the top 10 'superbugs' worldwide and is causing infections with poor outcomes in both humans and animals. From 202 P. aeruginosa isolates (n = 121 animal and n = 81 human), 40 were selected on the basis of biofilm-forming ability and were comparatively characterized in terms of virulence determinants to the type strain P. aeruginosa PAO1. Biofilm formation, pyocyanin and hemolysin production, and bacterial motility patterns were compared with the ability to kill human cell line A549 in vitro. On average, there was no significant difference between levels of animal and human cytotoxicity, while human isolates produced higher amounts of pyocyanin, hemolysins and showed increased swimming ability. Non-parametric statistical analysis identified the highest positive correlation between hemolysis and the swarming ability. For the first time an ensemble machine learning approach used on the in vitro virulence data determined the highest relative predictive importance of the submerged biofilm formation for the cytotoxicity, as an indicator of the infection ability. The findings from the in vitro study were validated in vivo using zebrafish (Danio rerio) embryos. This study highlighted no major differences between P. aeruginosa species isolated from animal and human infections and the importance of pyocyanin production in cytotoxicity and infection ability. © FEMS 2018.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173048/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173001/RS//
dc.rightsrestrictedAccess
dc.sourcePathogens and Disease
dc.subjectPseudomonas aeruginosaen
dc.subjectbiofilmen
dc.subjectvirulence factorsen
dc.subjectinfectionen
dc.subjectanimal isolatesen
dc.subjecthuman isolatesen
dc.titleBiofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humansen
dc.typearticleen
dc.rights.licenseARR
dcterms.abstractНикодиновић-Рунић, Јасмина; Васиљевић, Бранка; Сенеровић, Лидија; Миливојевић, Душан; Шумоња, Невен; Медић, Страхиња; Павић, Aлександар Б.; Морић, Ивана;
dc.rights.holder© FEMS 2018
dc.citation.volume76
dc.citation.issue4
dc.citation.spagefty041
dc.identifier.wos000439774500015
dc.identifier.doi10.1093/femspd/fty041
dc.citation.rankM23
dc.identifier.pmid29684116
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-85050798463


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