Determination of glucose using polyaniline modified electrode
2011
Autori
Jambrec, DaliborkaGvozdenović, Milica M.
Jugović, Branimir
Ostala autorstva
Ignjatović, Nenad L.Konferencijski prilog
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Sensor electrode was formed by immobilization of glucose oxidase (GOx) on polyaniline (PANI), electrochemically synthesized on graphite electrode from aqueous hydrochloride acid electrolyte containing aniline monomer by galvanostatic method. Optimization of the current density used for the synthesis of PANI was performed. Immobilization of GOx was achieved by crosslinking via glutaraldehyde and the efficiency of the immobilization was determined spectrophotometrically. Using chronoamperometric curves of glucose oxidation on polyaniline apparent Michaelis constant was estimated to be 0.273 mM. The storage stability of the enzyme electrode was examined for twenty days, after which it retained 84% of its initial signal.
Ključne reči:
glucose oxidase / polyaniline / electrochemistryIzvor:
Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia, 2011, 24-24Izdavač:
- Belgrade : Institute of Technical Sciences of the Serbian Academy of Sciences and Arts
Finansiranje / projekti:
- Elektrohemijska sinteza i karakterizacija nanostrukturiranih funkcionalnih materijala za primenu u novim tehnologijama (RS-MESTD-Basic Research (BR or ON)-172046)
Kolekcije
Institucija/grupa
VinčaTY - CONF AU - Jambrec, Daliborka AU - Gvozdenović, Milica M. AU - Jugović, Branimir PY - 2011 UR - http://dais.sanu.ac.rs/123456789/693 UR - https://vinar.vin.bg.ac.rs/handle/123456789/7515 AB - Sensor electrode was formed by immobilization of glucose oxidase (GOx) on polyaniline (PANI), electrochemically synthesized on graphite electrode from aqueous hydrochloride acid electrolyte containing aniline monomer by galvanostatic method. Optimization of the current density used for the synthesis of PANI was performed. Immobilization of GOx was achieved by crosslinking via glutaraldehyde and the efficiency of the immobilization was determined spectrophotometrically. Using chronoamperometric curves of glucose oxidation on polyaniline apparent Michaelis constant was estimated to be 0.273 mM. The storage stability of the enzyme electrode was examined for twenty days, after which it retained 84% of its initial signal. PB - Belgrade : Institute of Technical Sciences of the Serbian Academy of Sciences and Arts C3 - Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia T1 - Determination of glucose using polyaniline modified electrode SP - 24 EP - 24 UR - https://hdl.handle.net/21.15107/rcub_vinar_7515 ER -
@conference{ author = "Jambrec, Daliborka and Gvozdenović, Milica M. and Jugović, Branimir", year = "2011", abstract = "Sensor electrode was formed by immobilization of glucose oxidase (GOx) on polyaniline (PANI), electrochemically synthesized on graphite electrode from aqueous hydrochloride acid electrolyte containing aniline monomer by galvanostatic method. Optimization of the current density used for the synthesis of PANI was performed. Immobilization of GOx was achieved by crosslinking via glutaraldehyde and the efficiency of the immobilization was determined spectrophotometrically. Using chronoamperometric curves of glucose oxidation on polyaniline apparent Michaelis constant was estimated to be 0.273 mM. The storage stability of the enzyme electrode was examined for twenty days, after which it retained 84% of its initial signal.", publisher = "Belgrade : Institute of Technical Sciences of the Serbian Academy of Sciences and Arts", journal = "Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia", title = "Determination of glucose using polyaniline modified electrode", pages = "24-24", url = "https://hdl.handle.net/21.15107/rcub_vinar_7515" }
Jambrec, D., Gvozdenović, M. M.,& Jugović, B.. (2011). Determination of glucose using polyaniline modified electrode. in Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia Belgrade : Institute of Technical Sciences of the Serbian Academy of Sciences and Arts., 24-24. https://hdl.handle.net/21.15107/rcub_vinar_7515
Jambrec D, Gvozdenović MM, Jugović B. Determination of glucose using polyaniline modified electrode. in Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia. 2011;:24-24. https://hdl.handle.net/21.15107/rcub_vinar_7515 .
Jambrec, Daliborka, Gvozdenović, Milica M., Jugović, Branimir, "Determination of glucose using polyaniline modified electrode" in Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia (2011):24-24, https://hdl.handle.net/21.15107/rcub_vinar_7515 .