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Development of RAST assay for determination of anti-Populus canadensis IgE antibodies

dc.creatorTrtić, Tatjana M.
dc.creatorVuksanovic, L
dc.creatorParanos, S
dc.creatorPetrovic, S
dc.creatorVujčić, Zoran
dc.creatorPlećaš, Ilija B.
dc.creatorJankov, Ratko M.
dc.date.accessioned2018-03-03T15:10:50Z
dc.date.available2018-03-03T15:10:50Z
dc.date.issued1996
dc.identifier.issn0236-5731
dc.identifier.urihttps://vinar.vin.bg.ac.rs/handle/123456789/7219
dc.description.abstractOne of the frequent causes of pollen allergy in our region (Serbia, Yugoslavia) is the pollen of poplar (Populus canadensis). The aim of this study was to form RAST for the determination of specific anti-Populus canadensis IgE antibodies. Affinity purified and radiolabelled (I-125) MoAb El was used for forming assay for the determination of specific IgE. By titration of extract of poplar Populus canadensis we determined that the quantity of 0.65 mt extract is needed for coupling of ig BrCN activated paper discs. Coupling was performed in Na(2)CO3/NaHCO3 buffer pH 10. on 4 degrees C for 48h. Using this newely formed RAST, specific for Populus canadensis we have deteminated anti- Populus canadensis IgE antibodies as well. as cross reactivity between pollens of Populus canadensis and Populus deltoides.en
dc.rightsrestrictedAccessen
dc.sourceJournal of Radioanalytical and Nuclear Chemistryen
dc.titleDevelopment of RAST assay for determination of anti-Populus canadensis IgE antibodiesen
dc.typearticleen
dcterms.abstractТртиц, Т; Вујциц, З; Јанков, РМ; Плецас, И; Петровиц, С; Паранос, С; Вуксановиц, Л;
dc.citation.volume206
dc.citation.issue1
dc.citation.spage145
dc.citation.epage149
dc.identifier.wosA1996VD12500016
dc.identifier.doi10.1007/BF02040051
dc.description.otherInternational Conference on Isotopes, May 07-12, 1995, Beijing, Peoples R Chinaen
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-0029834625


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