Effect of EDTA on copper-induced inhibition of rat myometrial ecto-ATPase activity
Drakulić, Dunja R.
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The aim of this study was to examine in vitro chelators ability to prevent copper-induced inhibition of rat myometrial ecto-ATPase activity. The effects of increasing CuSO(4) concentrations, in the absence and presence of 1 mmol/l EDTA, showed sigmoidal and complete inhibition relative to the control enzyme activity. IC(50) values, 1.15 x 10(-4) and 1.71 x 10(-3) mol/l in the absence and presence of EDTA, respectively, were determined by Hill analysis from experimental curves. According to the results presented in this work, 1 mmol/l EDTA increased by one order of magnitude CuSO(4) concentration for half-maximal inhibition (IC(50)), by decreasing Cu(2+) concentrations, available to form inactive CuATP(2-) complex.
Source:Russian Journal of Physical Chemistry A, 2009, 83, 9, 1592-1595
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