Time-dependent inhibition of Na+/K+-ATPase induced by single and simultaneous exposure to lead and cadmium
AuthorsVasić, Vesna M.
Čolović, Mirjana B.
Vujačić, Ana V.
Stojić, Dragica Lj.
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Time-dependent interactions of Na+/K+-ATPase, isolated from rat brain synaptic plasma membranes (SPMs), with Cd2+ and Pb2+ ions in a single exposure and in a mixture were investigated in vitro. The interference of the enzyme with these metal ions was studied as a function of different protein concentrations and exposure time. The aim of the work was to investigate the possibility of selective recognition of Cd2+ and Pb2+ ions in a mixture, on the basis of the different rates of their protein-ligand interactions. Decreasing protein concentration increased the sensitivity of Na+/K+-ATPase toward both metals. The selectivity in protein-ligand interactions was obtained by variation of preincubation time (incubation before starting the enzymatic reaction).
Source:Russian Journal of Physical Chemistry A, 2007, 81, 9, 1402-1406
- 8th International Conference on Fundamental and Applied Aspects of Physical Chemistry, Sep 26-29, 2006, Belgrade, Serbia