The aim of this work is the in vitro study of human melanoma cell growth modulation after irradiation with protons. Confluent cell monolayers were irradiated with single doses ranging from 1 to 20 Gy, using proton beams having energy of 22.6 MeV at the target. 48 hours after irradiation, cell growth, cell cycle analyses and initiation of cell death were followed. The obtained results were compared with the effects of glucocorticoid hormones. The inhibition of melanoma cell growth was observed, especially after single application of 12 and 16 Gy. Cell cycle analyses of melanomas after proton irradiation, have shown the G2/M arrest of irradiated cells corresponding with the increase of applied dose. The flow cytometric analysis has shown presence of apoptotic nuclei. Glucocorticoid treatment has shown modest melanoma cell growth inhibition, cell cycle arrest in G2/M phase and ladder pattern on agarose gel electrophoresis.
TY - JOUR
AU - Ristić-Fira, Aleksandra
AU - Todorović, Danijela V.
AU - Petrović, Ivan M.
AU - Ruzvdijic, S
AU - Raffaele, L
AU - Sabini, MG
AU - Cirrone, Giuseppe Antonio Pablo
AU - Cuttone, Giacomo
AU - Farruggia, G
AU - Masotti, L
AU - Kanazir, Dušan T.
PY - 2001
UR - https://vinar.vin.bg.ac.rs/handle/123456789/6321
AB - The aim of this work is the in vitro study of human melanoma cell growth modulation after irradiation with protons. Confluent cell monolayers were irradiated with single doses ranging from 1 to 20 Gy, using proton beams having energy of 22.6 MeV at the target. 48 hours after irradiation, cell growth, cell cycle analyses and initiation of cell death were followed. The obtained results were compared with the effects of glucocorticoid hormones. The inhibition of melanoma cell growth was observed, especially after single application of 12 and 16 Gy. Cell cycle analyses of melanomas after proton irradiation, have shown the G2/M arrest of irradiated cells corresponding with the increase of applied dose. The flow cytometric analysis has shown presence of apoptotic nuclei. Glucocorticoid treatment has shown modest melanoma cell growth inhibition, cell cycle arrest in G2/M phase and ladder pattern on agarose gel electrophoresis.
T2 - Physica Medica
T1 - Inhibition of human melanoma cell growth by proton irradiation
VL - 17
SP - 71
EP - 75
ER -
@article{
author = "Ristić-Fira, Aleksandra and Todorović, Danijela V. and Petrović, Ivan M. and Ruzvdijic, S and Raffaele, L and Sabini, MG and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Farruggia, G and Masotti, L and Kanazir, Dušan T.",
year = "2001",
abstract = "The aim of this work is the in vitro study of human melanoma cell growth modulation after irradiation with protons. Confluent cell monolayers were irradiated with single doses ranging from 1 to 20 Gy, using proton beams having energy of 22.6 MeV at the target. 48 hours after irradiation, cell growth, cell cycle analyses and initiation of cell death were followed. The obtained results were compared with the effects of glucocorticoid hormones. The inhibition of melanoma cell growth was observed, especially after single application of 12 and 16 Gy. Cell cycle analyses of melanomas after proton irradiation, have shown the G2/M arrest of irradiated cells corresponding with the increase of applied dose. The flow cytometric analysis has shown presence of apoptotic nuclei. Glucocorticoid treatment has shown modest melanoma cell growth inhibition, cell cycle arrest in G2/M phase and ladder pattern on agarose gel electrophoresis.",
journal = "Physica Medica",
title = "Inhibition of human melanoma cell growth by proton irradiation",
volume = "17",
pages = "71-75"
}
Ristić-Fira, A., Todorović, D. V., Petrović, I. M., Ruzvdijic, S., Raffaele, L., Sabini, M., Cirrone, G. A. P., Cuttone, G., Farruggia, G., Masotti, L.,& Kanazir, D. T.. (2001). Inhibition of human melanoma cell growth by proton irradiation. in Physica Medica, 17, 71-75.
Ristić-Fira A, Todorović DV, Petrović IM, Ruzvdijic S, Raffaele L, Sabini M, Cirrone GAP, Cuttone G, Farruggia G, Masotti L, Kanazir DT. Inhibition of human melanoma cell growth by proton irradiation. in Physica Medica. 2001;17:71-75..
Ristić-Fira, Aleksandra, Todorović, Danijela V., Petrović, Ivan M., Ruzvdijic, S, Raffaele, L, Sabini, MG, Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Farruggia, G, Masotti, L, Kanazir, Dušan T., "Inhibition of human melanoma cell growth by proton irradiation" in Physica Medica, 17 (2001):71-75.
